Analysis of the cytotoxicity of differentially sized titanium dioxide nanoparticles in murine MC3T3-E1 preosteoblasts

Zhang, Yilin; Yu, Weiqiang; Jiang, Xinquan; Lv, Kaige; Sun, Shengjun; Zhang, Fuqiang

doi:10.1007/s10856-011-4375-7

Cited by 42 publications

(42 citation statements)

References 62 publications

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“…Superparamagnetic iron oxide nanoparticles used for stem cell tracking and magnetic resonance imaging impair the chondrogenic and osteogenic differentiation of human mesenchymal stem cells by altering the intracellular cytokine production profile of the cells, 375 and TiO 2 nanoparticles induce cytotoxicity in murine MC3T3-E1 preosteoblasts. 376 In summary, bone formation is a well-orchestrated process that involves many cell types and signaling pathways. Recent investigation has shown that nanoparticles are promising for use in bone tissue engineering.…”

Section: Nanotoxicity To Bonesmentioning

confidence: 99%

Perturbation of physiological systems by nanoparticles

et al. 2014

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Nanotechnology is having a tremendous impact on our society. However, societal concerns about human safety under nanoparticle exposure may derail the broad application of this promising technology. Nanoparticles may enter the human body via various routes, including respiratory pathways, the digestive tract, skin contact, intravenous injection, and implantation. After absorption, nanoparticles are carried to distal organs by the bloodstream and the lymphatic system. During this process, they interact with biological molecules and perturb physiological systems. Although some ingested or absorbed nanoparticles are eliminated, others remain in the body for a long time. The human body is composed of multiple systems that work together to maintain physiological homeostasis. The unexpected invasion of these systems by nanoparticles disturbs normal cell signaling, impairs cell and organ functions, and may even cause pathological disorders. This review examines the comprehensive health risks of exposure to nanoparticles by discussing how nanoparticles perturb various physiological systems as revealed by animal studies. The potential toxicity of nanoparticles to each physiological system and the implications of disrupting the balance among systems are emphasized.

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Section: Nanotoxicity To Bonesmentioning

confidence: 99%

Perturbation of physiological systems by nanoparticles

et al. 2014

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“…For example, TiO 2 NPs could induce DNA double-strand breaks in bone-marrow cells after oral administration. 4 Zhang et al 5 observed that TiO 2 NPs stimulated pro-inflammatory gene expression in pro-osteoblast cells (MC3T3-E1). Wang et al 6 indicated that TiO 2 NPs are potentially toxic to major organs and cause damage to the knee joints in rabbits.…”

Section: Introductionmentioning

confidence: 99%

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

Niska¹,

Pyszka²,

Tukaj³

et al. 2015

IJN

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Titanium dioxide (TiO 2 ) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO 2 NPs are frequently used as a material for orthopedic implants. However, to the best of our knowledge, the biocompatibility of TiO 2 NPs and their effects on osteoblast cells, which are responsible for the growth and remodeling of the human skeleton, have not been thoroughly investigated. In the research reported here, we studied the effects of exposing hFOB 1.19 human osteoblast cells to TiO 2 NPs (5–15 nm) for 24 and 48 hours. Cell viability, alkaline phosphatase (ALP) activity, cellular uptake of NPs, cell morphology, superoxide anion (O 2 •−2 ) generation, superoxide dismutase (SOD) activity and protein level, sirtuin 3 (SIR3) protein level, correlation between manganese (Mn) SOD and SIR, total antioxidant capacity, and malondialdehyde were measured following exposure of hFOB 1.19 cells to TiO 2 NPs. Exposure of hFOB 1.19 cells to TiO 2 NPs resulted in: (1) cellular uptake of NPs; (2) increased cytotoxicity and cell death in a time- and concentration-dependent manner; (3) ultrastructure changes; (4) decreased SOD and ALP activity; (5) decreased protein levels of SOD1, SOD2, and SIR3; (6) decreased total antioxidant capacity; (7) increased O 2 •− generation; and (8) enhanced lipid peroxidation (malondialdehyde level). The linear relationship between the protein level of MnSOD and SIR3 and between O 2 •− content and SIR3 protein level was observed. Importantly, the cytotoxic effects of TiO 2 NPs were attenuated by the pretreatment of hFOB 1.19 cells with SOD, indicating the significant role of O 2 •− in the cell damage and death observed. Thus, decreased expression of SOD leading to increased oxidizing stress may underlie the nanotoxic effects of TiO 2 NPs on human osteoblasts.

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“…On the one hand, hydroxyapatite and titania nanoparticles of up to 40 nm diameter decreased osteoblastic cell proliferation and viability, respectively. 99,116 On the other hand, in another study, only hydroxyapatite nanoparticles with a diameter of 20 nm enhanced cell growth of osteoblast-like cells compared with larger particles with a diameter of 80 nm. 117 In addition to particle uptake by osteoblasts and potential effects on proliferation and viability, the consequences of nanoparticle presence on the differentiation and mineralization of osteoblastic cells were assessed in a variety of studies with a wide range of particles.…”

Section: Osteoblastsmentioning

confidence: 92%

“…123 Similar osteoclastogenic effects were triggered in osteoblastic cells by titania nanoparticles via increased gene expression of granulocyte colony-stimulating factor. 116 In contrast, phosphonate-functionalized nanoparticles did not affect the expression of osteoclast-regulating genes in primary human osteoblasts. 112 Further examined effects of nanoparticles on osteoblasts comprised DNA damage, confirmed for hydroxyapatite nanoparticles in osteoblastic cells, 115 and titania particles in various murine organs, including bone marrow.…”

Section: Osteoblastsmentioning

confidence: 95%

“…66,112 In addition to potential inflammation, further severe effects such as DNA damage or initiation of apoptosis can be elicited by nanoparticles in osteoclast precursors. 116,146 In short, osteoclasts originate from the same precursors as other phagocytizing cells, such as macrophages, and readily take up nanoparticles. Arising clinical issues include aseptic implant loosening due to excessive osteoclast activity potentially caused by implant wear in the form of particles.…”

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confidence: 99%

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Nanoparticles and their potential for application in bone

Tautzenberger¹,

Kovtun²,

Ignatius³

2012

IJN

161

137

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Abstract:Biomaterials are commonly applied in regenerative therapy and tissue engineering in bone, and have been substantially refined in recent years. Thereby, research approaches focus more and more on nanoparticles, which have great potential for a variety of applications. Generally, nanoparticles interact distinctively with bone cells and tissue, depending on their composition, size, and shape. Therefore, detailed analyses of nanoparticle effects on cellular functions have been performed to select the most suitable candidates for supporting bone regeneration. This review will highlight potential nanoparticle applications in bone, focusing on cell labeling as well as drug and gene delivery. Labeling, eg, of mesenchymal stem cells, which display exceptional regenerative potential, makes monitoring and evaluation of cell therapy approaches possible. By including bioactive molecules in nanoparticles, locally and temporally controlled support of tissue regeneration is feasible, eg, to directly influence osteoblast differentiation or excessive osteoclast behavior. In addition, the delivery of genetic material with nanoparticulate carriers offers the possibility of overcoming certain disadvantages of standard protein delivery approaches, such as aggregation in the bloodstream during systemic therapy. Moreover, nanoparticles are already clinically applied in cancer treatment. Thus, corresponding efforts could lead to new therapeutic strategies to improve bone regeneration or to treat bone disorders.

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Analysis of the cytotoxicity of differentially sized titanium dioxide nanoparticles in murine MC3T3-E1 preosteoblasts

Cited by 42 publications

References 62 publications

Perturbation of physiological systems by nanoparticles

Perturbation of physiological systems by nanoparticles

Titanium dioxide nanoparticles enhance production of superoxide anion and alter the antioxidant system in human osteoblast cells

Nanoparticles and their potential for application in bone

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