Analysis of the Comprehensive Tensile Relationship in Electrospun Silk Fibroin/Polycaprolactone Nanofiber Membranes

Yin, Yunlei; Pu, Dandan; Xiong, Jie

doi:10.3390/membranes7040067

Cited by 16 publications

(13 citation statements)

References 36 publications

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“…Besides, it should be noted that the formation of intermolecular interaction between SFs and gelatin rendered the structural integrity of nanofiber mats and yielded the increase in mechanical properties (Zhu et al, 2015;Du et al, 2016;Selvaraj and Fathima, 2017). Moreover, according to morphological changes, higher gelatin content appeared to give rise to higher crosslinking density with resulting fiber fusion, thereby decreasing the porosity and increasing fiber entanglement leading to the strength enhancement (Simonet et al, 2014;Yin et al, 2017).…”

Section: Contact Angle and Water Uptake Capacitymentioning

confidence: 99%

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Mohammadzadehmoghadam

Dong

2019

Front. Mater.

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Bombyx mori silk fibroin (SF) /gelatin nanofiber mats with different blend ratios of 100/0, 90/10, and 70/30 were prepared by electrospinning and crosslinked with glutaraldehyde (GTA) vapor at room temperature. GTA was shown to induce the conformational transition of SFs from random coils to β sheets along with increasing nanofiber diameters with the addition of gelatin into SFs. It was found that by increasing the gelatin content, crosslinking degree was enhanced from 34% for pure SF nanofiber mats to 43% for SF/gelatin counterparts at the blend ratio of 70/30, which directly affected mechanical properties, porosity, and water uptake capacity (WUC) of prepared nanofiber mats. The addition of 10 and 30 wt% gelatin into SFs improved tensile strengths of SF/gelatin nanofiber mats by 10 and 27% along with moderate increases in Young's modulus by 12 and 27%, respectively, as opposed to plain SF counterparts. However, both porosity and WUC were found to decrease from 62 to 405% for pristine SF nanofiber mats to 47 and 232% for SF/gelatin counterparts at the blend ratio of 70/30 accordingly. To further evaluate the combined effect of GTA crosslinking and gelatin content on biological response of SF/gelatin scaffolds, the proliferation assay using 3T3 mouse fibroblast was conducted. In comparison with pure SFs, cell proliferation rate was lower for SF/gelatin constructs, which declined when the gelatin content increased. These results indicated that the adverse effect of GTA crosslinking on cell response may be ascribed to imposed changes in morphology and physiochemical properties of SF/gelatin nanofiber mats. Although crosslinking could be used to improve mechanical properties of nanofiber mats, it reduced their capacity to support the cell activity. GTA optimization is required to further modulate the physico-chemical properties of SF/gelatin nanofiber mats in order to obtain stable materials with favorable bioactive properties and promote cellular responses for tissue engineering applications.

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Section: Contact Angle and Water Uptake Capacitymentioning

confidence: 99%

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Mohammadzadehmoghadam

Dong

2019

Front. Mater.

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“…The initial toe region is attributed to the stretching of coiled collagen fibers, which is followed by a second stage where further elongation of the straightened collagen fibers results in a steep rise in tensile stress until the tissue attains maximum extensibility . On the other hand, polymeric scaffolds showed a relatively linear mechanical behavior as shown by us (Figure F,G) and others . This could be attributed to the relatively straight electrospun fibers as observed by SEM (Figure C) and absence of undulation, which are found in the wavy collagen fibers of native heart valves.…”

Section: Resultsmentioning

confidence: 51%

“…31 On the other hand, polymeric scaffolds showed a relatively linear mechanical behavior as shown by us ( Figure 1F,G) and others. 31,[39][40][41][42] This could be attributed to the relatively straight electrospun fibers as observed by SEM ( Figure 1C) and absence of undulation, which are found in the wavy collagen fibers of native heart valves. Another reason of this nonlinear behavior of native valves could be the higher degree of heterogeneity of native valve ECM fibers than synthetic polymers, which have varying fiber lengths and crosslinking percentages.…”

Section: Fabrication Of Anisotropic Fibrous Scaffoldsmentioning

confidence: 71%

Valve leaflet‐inspired elastomeric scaffolds with tunable and anisotropic mechanical properties

Xue

Ravishankar

Zeballos

et al. 2019

Polymers for Advanced Techs

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Fibrous scaffolds, which can mimic the elastic and anisotropic mechanical properties of native tissues, hold great promise in recapitulating the native tissue microenvironment. We previously fabricated electrospun fibrous scaffolds made of hybrid synthetic elastomers (poly(1,3‐diamino‐2‐hydroxypropane‐co‐glycerol sebacate)‐co‐poly (ethylene glycol) (APS‐co‐PEG) and polycaprolactone (PCL)) to obtain uniaxial mechanical properties similar to those of human aortic valve leaflets. However, conventional electrospinning process often yields scaffolds with random alignment, which fails to recreate the anisotropic nature of most of the soft tissues such as native heart valves. Inspired by the structure of native valve leaflet, we designed a novel valve leaflet‐inspired ring‐shaped collector to modulate the electrospun fiber alignment and studied the effect of polymer formulation (PEG amount [mole %] in APS‐co‐PEG; ratio between APS‐co‐PEG and PCL; and total polymer concentration) in tuning the biaxial mechanical properties of the fibrous scaffolds. The fibrous scaffolds collected on the ring‐shaped collector displayed anisotropic biaxial mechanical properties, suggesting that their biaxial mechanical properties are closely associated with the fiber alignment in the scaffold. Additionally, the scaffold stiffness was easily tuned by changing the composition and concentration of the polymer blend. Human valvular interstitial cells (hVICs) cultured on these anisotropic scaffolds displayed aligned morphology as instructed by the fiber alignment. Overall, we generated a library of biologically relevant fibrous scaffolds with tunable mechanical properties, which will guide the cellular alignment.

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“…It has been shown that lateral contraction of nonwoven mats is ascribable to the buckling of fibers that are initially perpendicular or laterally oriented with respect to the loading direction, whereas fibers oriented along the loading axis undergo further alignment [ 38 ]. The mechanical properties of fibrous membranes thus depend on both the mechanical properties of the single fibers making up the membrane and the structure of the membrane itself, for example, how tight the fibers are packed and interconnected, and whether the fibers show any preferential orientation [ 38 – 40 ].…”

Section: Resultsmentioning

confidence: 99%

Potential of Electrospun Poly(3-hydroxybutyrate)/Collagen Blends for Tissue Engineering Applications

Salvatore

Carofiglio²,

Stufano³

et al. 2018

Journal of Healthcare Engineering

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In this work, tunable nonwoven mats based on poly(3-hydroxybutyrate) (PHB) and type I collagen (Coll) were successfully produced by electrospinning. The PHB/Coll weight ratio (fixed at 100/0, 70/30, and 50/50, resp.) was found to control the morphological, thermal, mechanical, and degradation properties of the mats. Increasing collagen amounts led to larger diameters of the fibers (in the approximate range 600–900 nm), while delaying their thermal decomposition (from 245°C to 262°C). Collagen also accelerated the hydrolytic degradation of the mats upon incubation in aqueous medium at 37°C for 23 days (with final weight losses of 1%, 15%, and 23% for 100/0, 70/30, and 50/50 samples, resp.), as a result of increased mat wettability and reduced PHB crystallinity. Interestingly, 70/30 meshes were the ones displaying the lowest stiffness (~116 MPa; p < 0.05 versus 100/0 and 50/50 meshes), while 50/50 samples had an elastic modulus comparable to that of 100/0 ones (~250 MPa), likely due to enhanced physical crosslinking of the collagen chains, at least at high protein amounts. All substrates were also found to allow for good viability and proliferation of murine fibroblasts, up to 6 days of culture. Collectively, the results evidenced the potential of as-spun PHB/Coll meshes for tissue engineering applications.

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Analysis of the Comprehensive Tensile Relationship in Electrospun Silk Fibroin/Polycaprolactone Nanofiber Membranes

Cited by 16 publications

References 36 publications

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Fabrication and Characterization of Electrospun Silk Fibroin/Gelatin Scaffolds Crosslinked With Glutaraldehyde Vapor

Valve leaflet‐inspired elastomeric scaffolds with tunable and anisotropic mechanical properties

Potential of Electrospun Poly(3-hydroxybutyrate)/Collagen Blends for Tissue Engineering Applications

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