Analysis of the complete genome sequence of two Korean sacbrood viruses in the Honey bee, Apis mellifera

Choe, Se Eun; Nguyen, Lien T.; Noh, Jin Hyeong; Kweon, Chang-Hee; Reddy, Kondreddy E.; Koh, Hong Bum; Chang, Ki Yoon; Kang, Seung Won

doi:10.1016/j.virol.2012.06.008

Cited by 44 publications

(48 citation statements)

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“…In addition, it is unusual for SBV infection in A. mellifera colonies to cause colony death (22). However, SBV is deadly to Asian honeybees, and serious mortality rates for A. cerana due to SBV infection have been reported in Vietnam, Thailand, India, China, and South Korea (7,(23)(24)(25)(26). Large-scale A. cerana colony losses caused by SBV infection were first reported in Guangdong Province, China, in 1972 (27).…”

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confidence: 99%

Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera

Gong

Chen

Chen³

et al. 2016

Appl Environ Microbiol

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bSacbrood virus (SBV) is one of the most destructive viruses in the Asian honeybee Apis cerana but is much less destructive in Apis mellifera. In previous studies, SBV isolates infecting A. cerana (AcSBV) and SBV isolates infecting A. mellifera (AmSBV) were identified as different serotypes, suggesting a species barrier in SBV infection. In order to investigate this species isolation, we examined the presence of SBV infection in 318 A. mellifera colonies and 64 A. cerana colonies, and we identified the genotypes of SBV isolates. We also performed artificial infection experiments under both laboratory and field conditions. The results showed that 38 A. mellifera colonies and 37 A. cerana colonies were positive for SBV infection. Phylogenetic analysis based on RNA-dependent RNA polymerase (RdRp) gene sequences indicated that A. cerana isolates and most A. mellifera isolates formed two distinct clades but two strains isolated from A. mellifera were clustered with the A. cerana isolates. In the artificial-infection experiments, AcSBV negative-strand RNA could be detected in both adult bees and larvae of A. mellifera, although there were no obvious signs of the disease, demonstrating the replication of AcSBV in A. mellifera. Our results suggest that AcSBV is able to infect A. mellifera colonies with low prevalence (0.63% in this study) and pathogenicity. This work will help explain the different susceptibilities of A. cerana and A. mellifera to sacbrood disease and is potentially useful for guiding beekeeping practices.

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confidence: 99%

Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera

Gong

Chen

Chen³

et al. 2016

Appl Environ Microbiol

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“…CSBV-SX contained a base composition very similar to that of other SBV strains, including CSBV-BJ, CSBV-GZ, SBV-UK, and SBV-19 (Ghosh et al, 1999;Grabensteiner et al, 2001;Zhang et al, 2001;Chen et al, 2006;Mingxiao et al, 2011;Choe et al, 2012). Genomic alignment analysis showed that CSBV-SX shared high homology with other SBV strains (88.3-97.5%), and that it was classified into the same group as the CSBV-BJ strain in the complete genomic phylogenetic analysis.…”

Section: Discussionmentioning

confidence: 85%

“…Reverse transcription-PCR was successfully used to identify the RNA genomic virus infection, and nested or seminested PCR was used in these identification procedures (Grabensteiner et al, 2001;Choe et al, 2012;Reddy et al, 2013). For nested PCR or seminested PCR, at least 2 rounds of PCR amplification were needed to complete and verify the identification, which were followed by nucleotide sequencing.…”

Section: Discussionmentioning

confidence: 99%

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Genomic characterization and phylogenetic analysis of Chinese sacbrood virus isolated from Loess Plateau, China

Liu

Wang

2016

Genet. Mol. Res.

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ABSTRACT. The complete genomic RNA of the Chinese sacbrood virus (CSBV) strain, which infects the honeybees in the Loess plateau, was sequenced and analyzed. The CSBV-SX strain contains 8705 nucleotides, which includes a single large open reading frame (99-8681 nucleotides) encoding 2860 amino acids. A novel efficient identification method was used to investigate the samples infected by CSBV. The putative amino acid sequence alignment analysis showed that, except for some normal well characterized domains such as RNA helicase, RNA protease, and RNA-dependent RNA polymerase domains, a calicivirus coat protein domain was identified at amino acids 493-564. Phylogenetic analysis indicated that CSBV-SX was closely related to CSBV-BJ, and this result was supported by nucleotide multiple sequence alignment and protein multiple 2 H. Yu et al. Genetics and Molecular Research 15 (3): gmr.15036928 sequence alignment analysis results. These differences in the CSBV-SX strain may be related to virus adaptation to the xerothermic, low relative humidity, and strong ultraviolet radiation conditions in the Loess Plateau.

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“…The filtered Illumina short reads were then mapped to A. mellifera reference mRNA sequences (ftp://ftp.ncbi.nih.gov/ of SBV infected adults is low while SBV results significant larval mortality (Bailey and Fernando, 1972) which suggests that the responses of the hosts against SBV might be different to each other according to their developmental stage and/ or caste. Recently, the SBV isolated from A. cerana in Korea was sequenced, and characterized by molecular genomics and phylogenetic approach to reveal that the SBV found in Korea is different from other strains previously published (Choe et al, 2012a(Choe et al, , 2012b, however, the molecular biology and nucleotide sequence information of A. cerana, which is very important to study the virus and host interaction, is still limited. Currently, (Mardis, 2008;Velculescu et al, 1995).…”

Section: Analysis Of Transcriptome Sequencing Resultsmentioning

confidence: 99%

Comparative Transcriptome Analysis of Queen, Worker, and Larva of Asian Honeybee, Apis cerana

Kim¹,

Lee²,

An³

et al. 2013

International Journal of Industrial Entomology

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The Asian honeybee, Apis cerana, is a native honeybee species in Korea which is important in agriculture for pollination and honey production. For better understanding of the physiology of A. cerana, high-throughput Illumina transcriptome sequencing was performed to analyze the gene expression profiles of queen, worker, and larva. A total of 219,799,682 clean reads corresponding to 22.2 Gb of nucleotide sequences was obtained from the whole body total RNA samples. The Apis mellifera reference mRNA sequence database was used to measure the gene expression level with Bowtie2 and eXpress software, and the Illumina short reads were then mapped to 11,459 out of 11,736 A. mellifera reference genes. Total of 9,221 genes with FPKM value greater than 5 of each sample group were subjected to eggNOG with BLASTX for gene ontology analysis. The differential gene expression between queen and worker, and worker and larva were analyzed to screen the overexpressed genes in each sample group. In the queen and worker sample group, total of 1,766 genes were differentially expressed with 887 and 879 genes overexpressed over two folds in queen and worker, respectively. In the worker and larva sample group, total of 1,410 genes were differentially expressed with 1,009 and 401 genes overexpressed over two folds in worker and larva, respectively. IntroductionApis cerana, the Asian honeybee is a native bee species to eastern and southeastern Asian countries such as Korea, China, and Japan. In Korea, A. cerana is one of the most important honeybee species along with western honeybee, Apis mellifera because not only of the economic importance of honey production, but also the importance as one of the pollinator species in agriculture. Also A. cerana had been concerned of its strong resistance to the ectoparasitic mites (Peng et al., 1987).However, the recent outbreak of sacbrood virus (SBV) belonging to the genus Iflavirus which infects A. cerana (Choi et al., 2010) caused a devastating colony loss of Korean honeybee industry.SBV infected larva fails to pupate, and accumulates virus enriched ecdysal fluid beneath its unshed skin (Bailey et al., 1964). SBV also infects adult bees, however, the mortality

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Analysis of the complete genome sequence of two Korean sacbrood viruses in the Honey bee, Apis mellifera

Cited by 44 publications

References 23 publications

Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera

Evidence of Apis cerana Sacbrood virus Infection in Apis mellifera

Genomic characterization and phylogenetic analysis of Chinese sacbrood virus isolated from Loess Plateau, China

Comparative Transcriptome Analysis of Queen, Worker, and Larva of Asian Honeybee, Apis cerana

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