Analysis of the coding sequence and expression of the coiled-coil α-helical rod protein 1 gene in normal and neoplastic epithelial cervical cells

Abstract

Abstract. The role of the CCHCR1 (coiled-coil α-helical rod protein 1) protein in the cell is poorly understood. It is thought to be engaged in processes such as proliferation and differentiation of epithelial cells, tissue-specific gene transcription and steroidogenesis. It is supposed to participate in keratinocyte transformation. It has also been found that this protein interacts with the E2 protein of human papilloma virus type 16 (HPV16). The oncogenic HPV forms, such as HPV16, are known to be necessary but not sufficient agents in the development of cervical carcinoma. In the present study, the CCHCR1 gene coding sequence and its expression was analyzed in normal, precancerous and cervical cancer cells. Changes in the noncoding region were found in 20.3% of the examined probes from women with cervical cancer or precancerous lesions and in 16.67% of the control probes. Most of the detected changes were single nucleotide polymorphisms (SNPs). Changes in the coding region were found in 22.8% of the probes with cervical cancer and in 16.67% of the control probes and all of them were SNPs. The level of CCHCR1 transcripts was determined using the real-time PCR method and the highest gene expression was detected in the H-SIL group and slightly decreased in the cervical carcinoma cells, compared with the control probes. It suggests that CCHCR1 could have a role in the process of cervical epithelial cell transformation, but this suggestion must be confirmed experimentally.

Centrosomal Localization of the Psoriasis Candidate Gene Product, CCHCR1, Supports a Role in Cytoskeletal Organization

“…Furthermore, CCHCR1 mRNA expression is upregulated in SCC cultures when compared to normal keratinocytes. Recently, a similar increase in CCHCR1 expression was observed in neoplastic cervical H-SIL samples (high-grade squamous intraepithelial lesions) [13]. Interestingly, the strongest CCHCR1 expression in SCCs and BCCs is observed in areas positive for epidermal growth factor receptor (EGFR).…”

Identification of differential plasma miRNA profiles in Chinese workers with occupational lead exposure

“…Lead, along with many other toxic metals, is closely associated with epigenetic modifications in humans, including DNA methylation, histone deacetylation, and miRNA dysregulation [ 9 – 11 ]. These epigenetic changes might also influence gene expression by various mechanisms.…”

Do myeloproliferative neoplasms and multiple myeloma share the same genetic susceptibility loci?

“…Our bioinformatics analysis suggests that this polymorphism is functional, showing a score of 1f in RegulomeDB and that it is an eQTL for the gene CCHCR1 and the noncoding RNA HCG27 in GTEx in whole blood, which represents the closest tissue of interest available in GTEx for MPN. CCHCR1 encodes for a protein with five coiled‐coil alpha‐helical rod domains and is a regulator of cell cycle progression and cell proliferation; its aberrant expression was already associated with other human cancers 28‐31 . For the noncoding RNA HCG27 , no data are available in the literature in relation with cancer.…”

“…CCHCR1 encodes for a protein with five coiled-coil alpha-helical rod domains and is a regulator of cell cycle progression and cell proliferation; its aberrant expression was already associated with other human cancers. [28][29][30][31] For the noncoding RNA HCG27, no data are available in the literature in relation with cancer. A decreased risk of ET was observed for the allele C of ATG5-rs9373839.…”