Analysis of the chlorophyll catabolism pathway in leaves of an introgression senescence mutant of Lolium temulentum

Stay-green protein, defective in Mendel’s green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway

“…King, H. Thomas and L. Mur, unpublished), although there lower PAO activities had been observed as well (Roca et al, 2004). Finally, Ren et al (2007) also identified lower PAO activity in nye1, but unfortunately PAO protein abundance was not determined in this study.…”

“…1A). After 8d dark incubation, 84% of chl was retained in JI2775, thus showing a stronger retention than stay-green Lolium temulentum (Roca et al, 2004) or Arabidopsis nye1 (Ren et al, 2007). Increase in the chl a to b ratio is a characteristic of senescence-related chl breakdown (Pružinská et al, 2005).…”

“…SGR absence in JI2775 does not influence the PAO pathway Thomas et al, 1996;Roca et al, 2004;Ren et al, 2007). We re-addressed this assumption using JI4 and JI2775 and determining PAO abundance and activity.…”

“…T-DNA insertion was verified by sequencing a PCR product obtained with the left T-DNA border primer LBb1 (5'-GCGTGGACCGCTTGCTGCAAT-3') and an AtSGR2-specific primer (5'-GCAACTGGTGAAAGCAAGAAC-3'). A Lolium line carrying a sid gene introgression has been described (Roca et al, 2004) and was obtained from H. …”

“…The situation is different for sgr/sid mutants, since so far the function of SGR/SID could not unequivocally be demonstrated. Several early reports deal with the biochemical lesion of sid and sidlike mutants, including Mendel's I mutant Thomas et al, 1996;Roca et al, 2004). The cumulative results of these investigations indicated that SGR/SID affects PAO activity.…”