Analysis of the chemical profile of cerrado pear fixed compounds by mass spectrometry with paper spray and volatile ionization by SPME-HS CG-MS

Abstract

Eugenia klotzschiana Berg is a native fruit of the cerrado, belonging to the Myrtaceae family, known as the cerrado pear and very little explored. The present study's objective was to obtain the profile of the fixed and volatile chemical constituents of the fruit. Mass spectrometry with ambient ionization paper spray (PS-MS) was used in positive and negative mode for fixed compounds. For volatiles, solid-phase microextraction by head space and gas chromatography was employed. The PS-MS identified 31 compounds, 21 in the negative and 10 in the positive mode, including fatty acids, flavonoids, phenolic acids, tannins and sugars. The solid-phase microextraction method was used in head space mode, using the polydimethylsiloxane-divinylbenzene fiber to extract volatile organic compounds (VOCs). The volatiles detected totaled 11 substances, belonging to the class of monoterpenes, sesquiterpenes, and esters. PS-MS was capable and efficient in determining the fixed compounds present in the cerrado pear pulp, providing the chemical profile of the fruit, which contains essential compounds with potential antioxidant bioactivity. The volatile profile was obtained with success, indicating menthol, fruity and vanilla notes in the pulp of the cerrado pear.

Physicochemical Characterization and Paper Spray Mass Spectrometry Analysis of Myrciaria Floribunda (H. West ex Willd.) O. Berg Accessions

“…Three classes of acids were identified in the rumberry fruit, corresponding to the compounds oxo-dihydroxy eicosanoic acid (fatty acid), dimethyl ellagic acid hexoside (phenolic acid), and citric acid (carboxylic acid). The peak with m/z 311 and fragment m/z 293 was identified as eicosanoic acid, as with findings on pera do cerrado constituents (Eugenia klotzschiana) [29].…”

“… Compounds identified by other authors in different Myrtaceae species: [ 28 ] Cuadrado-Silva, Pozo-Bayón e Osorio. (2017); [ 24 ] Faria et al, (2011); [ 29 ] Mariano et al, (2020); [ 31 ] Mena et al, (2012); [ 23 ] Pereira et al, (2017); [ 27 ] Rodrigues et al, (2021); [ 32 ] Salvador et al, (2011); [ 25 ] Santos (2019); [ 30 ] Santos et al, (2018); [ 5 ] Silva et al, (2019); [ 21 ] Teixeira et al, (2015). …”

Análise comparativa do perfil de compostos orgânicos voláteis de pimenta rosa e de aroeira do sertão

“…et al, 2021. As vantagens desse método de extração estão associadas ao seu baixo custo analítico, simplicidade, ausência de solventes e pela alta capacidade de adsorção de diferentes classes de compostos resultante dos diversos tipos de revestimentos das fibras SPME e podendo ser empregado para analisar matrizes complexas como acerola (GARCÍA et al, 2019), cagaita (RODRIGUES et al, 2021SILVA et al, 2019;SILVA et al, 2020;), callistemo (OLIVEIRA JÚNIOR, 2020, cambuí (GARCÍA et al, 2021), cerveja (PEREIRA et al 2021), grumixama (RAMOS et al, 2020RAMOS et al, 2021), kiwi (ASSUNÇÃO et al, 2020, manga (RAMOS, et al, 2021a, RAMOS et al, 2021b, milho (BOTTI et al, 2019;FRANZIN et al, 2020;NASCIMENTO, P. T. et al, 2021), pequi (SANTOS et al, 2020), pera do cerrado (MARIANO et al, 2020), soja (ROCHA. et al, 2019;BUENO et al, 2021), uva (MAZZINGHY, A. C. et al, 2021VIANA et al, 2018).…”

Profile of the volatile organic compounds of pink pepper and black pepper

“…This method, developed by Arthur and Pawliszyn (1990), uses the principle of "green chemistry", since it requires a small amount of sample and does not use organic solvents, and can be used direct extraction mode or in headspace mode (HS-SPME). A great advantage of HS-SPME is its high capacity for adsorption of different classes of compounds, duo to the diversity of applicable coatings of SPME fibers, and it can be used to analyze complex matrices such as acerola, cagaita, callistemon, cambuí, beer, grumixama, pequi, corn, as well as spices such as black pepper and pink pepper, species which will be analyzed in this work (Franzin et al, 2020;Figueiredo et al, 2021;Srinivasan, 2008;García et al, 2019;Rodrigues et al, 2021;Silva et al, 2019;Silva et al, 2020;Oliveira Júnior, 2020;García et al, 2021;Pereira et al 2021;Ramos et al, 2020;Assunção et al, 2020;Ramos, et al, 2021a;Ramos et al, 2021b;Botti et al, 2019;Nascimento et al, 2021a;Nascimento et al, 2021b;Santos et al, 2020;Mariano et al, 2020;Rocha et al, 2019;Bueno et al, 2021;Mazzinghy et al, 2021;Viana et al, 2018).…”