Analysis of the C. elegans Germline Stem Cell Pool

Crittenden, Sarah L.; Seidel, Hannah S.; Kimble, Judith

doi:10.1007/978-1-0716-3259-8_1

Cited by 2 publications

(1 citation statement)

References 155 publications

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“…These contradictory findings suggest the effect of HSF-1 knockdown on the germ line is dosage or developmental stage dependent. Another method frequently used to assess germline proliferation is the mitotic index (MI), which is the percentage of PZ cells that are Mphase (Crittenden et al, 2023). In addition to hsf-1, we found silencing of F54F2.9 and nhr-146 caused changes in MI without changing the absolute number of pH3 + cells (Figure S5A-C).…”

Section: Transcriptional Control Of Distal Germ Cell Behaviormentioning

confidence: 89%

A Transcription Factor Functional Atlas of Germline Development

Pocock,

Cao,

Fan

et al. 2024

Preprint

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Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Transcription factors (TFs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire TF repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed TFs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 TFs with discrete autonomous germline functions. By identifying TFs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.

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Section: Transcriptional Control Of Distal Germ Cell Behaviormentioning

confidence: 89%

A Transcription Factor Functional Atlas of Germline Development

Pocock,

Cao,

Fan

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans

Cao,

Fan,

Amparado

et al. 2024

Nat Commun

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Fertility requires the faithful proliferation of germ cells and their differentiation into gametes. Controlling these cellular states demands precise timing and expression of gene networks. Nucleic acid binding proteins (NBPs) play critical roles in gene expression networks that influence germ cell development. There has, however, been no functional analysis of the entire NBP repertoire in controlling in vivo germ cell development. Here, we analyzed germ cell states and germline architecture to systematically investigate the function of 364 germline-expressed NBPs in the Caenorhabditis elegans germ line. Using germline-specific knockdown, automated germ cell counting, and high-content analysis of germ cell nuclei and plasma membrane organization, we identify 156 NBPs with discrete autonomous germline functions. By identifying NBPs that control the germ cell cycle, proliferation, differentiation, germline structure and fertility, we have created an atlas for mechanistic dissection of germ cell behavior and gamete production.

show abstract

Analysis of the C. elegans Germline Stem Cell Pool

Cited by 2 publications

References 155 publications

A Transcription Factor Functional Atlas of Germline Development

A Transcription Factor Functional Atlas of Germline Development

A nucleic acid binding protein map of germline regulation in Caenorhabditis elegans

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