Analysis of the Apolipoprotein B Gene 3' Hypervariable Region Among Nationals of the Abu Dhabi Emirate and Comparisons with Other Populations

Abstract

Background: Among all the polymorphic markers available to date, a hypervariable region located in the 3' of the human apolipoprotein B gene has been extensively studied in global populations throughout the world. Patients and Methods: Using a polymerase chain reaction-based assay, we investigated the allele and genotype frequency distributions of the alleles (corresponding to repeats of a 30 base-pair core DNA sequence) of this hypervariable region in a group of 367 unrelated nationals (201 males, 166 females) from the United Arab Emirates. Results: We found 18 different alleles, ranging from 21 to 55 repeats, making up 51 genotypes that occurred in Hardy-Weinberg proportions and were associated with a heterozygosity index of 80.9%. The allele frequency distribution was different from that of other populations in that it was trimodal, with peaks at 31, 37 and 47 repeats, with corresponding relative frequencies of 16.1%, 25.1% and 6.0%. A four-allele model, which allowed comparisons with other reports, revealed distribution differences with all other ethnic groups except South Asians and Serbs. Conclusion: This marker is very informative for the Emirati population, and will be very useful for UAE-specific DNA fingerprinting. It will also be a valuable tool for assessing the role of apolipoprotein B in cardiovascular diseases.

Association of DNA Polymorphism at the Apolipoprotein B-100 Gene Locus with Plasma Lipid Concentration and Coronary Artery Disease among North Indians

“…As mentioned in the earlier section that grouping of the alleles into different allelic model like five-allelic model proposed by Frossard and Lestringant, [25] , it was clear that group comprising of higher repeat alleles are more frequent in patients as compared to controls, an observation which was not so clearly visible when individual alleles were compared. Probable reason behind this trend lies in the high degree of allelic differentiation observed at ApoB3'HVR which might have hided the association signals among the two groups that belongs to same ethnicity, which became pronounced once these numerous alleles were grouped into three or five allelic structure.…”

“…E = EcoR1; M= MspI; X = XbaI * p = < 0.05, **p = <0.001 and *** p = < 0.0001 132 ± 35 120 ± 31 0.001 +, -refers to the presence and absence of cutting site for restriction endonuclease. E = EcoR1; M= MspI; X = XbaI * p = < 0.05, **p = <0.001 and *** p = < 0.0001 higher repeats (39-49), lower repeats (alleles (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33) and median repeats (34-37) genotypes. It was observed that in extension of the genotype analysis, a significant association (p = 0.03) was observed between the higher repeats HVE 39-49 alleles and higher levels of Apo B (Table 3).…”

A Prospective Genetic Marker of the Visual-Perception Disorder Meares-Irlen Syndrome

“…In contrast, the alleles AV3 and AV4 were relatively common to the controls but not to the MIS group. None of the 10 MIS subjects were homozygotes (p = .11, onesided, 95%CI = 0, 0.34, binomial distribution), whereas four of the eight control subjects (50%) were homozygous for alleles of APOB (p = .05, onesided, 95%CI = 0.17, 0.83, binomial distribution) but neither result was significantly different from the expected proportion of 0.2 (Boerwinkle, et al, 1989;Gene, Huguet, Sanchez-Garcia, Moreno, Corbella, & Mezquita, 1995;Frossard & Lestringant, 1999). However, the odds of the control group being homozygotes were significantly greater than the odds for the MIS group (p ≤ .03, 95%CI = 1.04, ∞, Fisher's exact test).…”

Chimerism monitoring following allogeneic hematopoietic stem cell transplantation

“…A total of 111 alleles were observed that ranged from seven to nine at seven STR loci (Tho1, TPO, FES, VWA, D4S243, DHFRP2 and VWF-1) whereas X11 alleles [11][12][13][14][15] were observed at the remaining three STR loci (D3S1358, D16S310 and F13A). In contrast, 16 alleles were observed only at ApoB 0 3HVR ( Table 1).…”

“…A total of 111 observed alleles with as many as 16 alleles were observed at ApoB3 0 HVR, which is in accordance with other studies. 15 STR and VNTR markers are the most accepted regions of the human genome for assessing the levels of genetic diversity owing to their high mutation rates and high degree of polymorphism. 16 We also calculated different diversity parameters in order to quantify the range of polymorphism and genetic diversity.…”