Analysis of the antimicrobial resistance gene frequency in whole-genome sequenced Vibrio from Latin American countries

Vilela, Felipe Pinheiro; Falcão, Juliana Pfrimer

doi:10.1099/jmm.0.001428

Cited by 3 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolate was susceptible to amoxicillin, sulfamethoxazole/trimethoprim, nitrofurantoin, norfloxacin, oxytetracycline, florfenicol, neomycin, and erythromycin. This was contrary to the typical antimicrobial resistance traits observed in other Brazilian isolates of V. vulnificus [12,42,43]. On the other hand, despite lincomycin having the lowest inhibition zone (8 mm), it was not possible to find an actual reference for breakpoints for appropriate comparison.…”

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 59%

First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil

Janampa-Sarmiento,

Reis,

Egger

et al. 2024

Fishes

View full text Add to dashboard Cite

Vibrio vulnificus is an opportunistic pathogen in humans and exhibits pathogenic behavior in several aquaculture fish species. To date, in Brazil, there are no reports of V. vulnificus outbreaks in farmed fish. However, in 2019, sorubim (Pseudoplatystoma sp.) in the grow-out phase with clinical signs and cumulative mortality of 40% was registered. We aimed to identify and characterize the etiological agents of this outbreak. Seven moribund fish were sampled for bacteriological studies, and isolates were obtained from the brain and kidneys. Bacterial identification using matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF/MS) revealed V. vulnificus. One isolate, RP4, was used for identification via dnaJ and 16S rRNA gene sequencing and antimicrobial susceptibility evaluation. Sorubim juveniles were experimentally challenged with RP4 isolate via intracelomic injection (IC, 107 colony-forming units [CFU] fish−1) and immersion bath (IB, 106 CFU mL−1 for 30 min). Identities to V. vulnificus of dnaJ and 16S rRNA genes by BLAST analysis were higher than 92% and 98%, respectively. Susceptibility to oxytetracycline, florfenicol, and other antimicrobial molecules was also observed. In the IC-challenged group, the mortality rate was 100% with V. vulnificus recovered from fish organs (brain, liver, spleen, and kidney). Additionally, splenic endothelium alterations were observed in the IC group. On the contrary, the control and IB groups did not develop any clinical signs, mortality, or bacterial recovery after 7 days of challenge. To the best of our knowledge, this is the first report of pathogenic V. vulnificus in farmed Pseudoplatystoma sp.

show abstract

Section: Antimicrobial Susceptibility Testingmentioning

confidence: 59%

First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil

Janampa-Sarmiento,

Reis,

Egger

et al. 2024

Fishes

View full text Add to dashboard Cite

show abstract

“…The largest number of Vibrio isolates exhibited genotypic resistance to varG and bla vcc−1 , which demonstrated activity against penicillin, cephalosporins, and carbapenems (Mangat et al, 2016;Lin et al, 2017). The high prevalence of the varG gene has been demonstrated in a recent study conducted on various Vibrio species isolates collected from human, food, animal and environmental sources in Latin America (Vilela and Falcão, 2021). Carbapenemase VCC-1, which produces nontoxigenic V. cholerae strains, was described for the first time by Canadian researchers in 2016 (Mangat et al, 2016).…”

Section: Antimicrobial Resistance Genes Profilementioning

confidence: 96%

Virulence and resistance patterns of Vibrio cholerae non-O1/non-O139 acquired in Germany and other European countries

Schmidt,

Scholz,

Appelt

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

Global warming has caused an increase in the emergence of Vibrio species in marine and estuarine environments as well as fresh water bodies. Over the past decades, antimicrobial resistance (AMR) has evolved among Vibrio species toward various antibiotics commonly used for the treatment of Vibrio infections. In this study, we assessed virulence and resistance patterns of Vibrio cholerae non-O1/non-O139 strains derived from Germany and other European countries. A total of 63 clinical and 24 environmental Vibrio cholerae non-O1/non-O139 strains, collected between 2011 and 2021, were analyzed. In silico antibiotic resistances were compared with resistance phenotypes according to EUCAST breakpoints. Additionally, genetic relatedness between isolates was assessed by two cgMLST schemes (SeqSphere +, pubMLST). Both cgMLST schemes yielded similar results, indicating high genetic diversity among V. cholerae non-O1/non-O139 isolates. Some isolates were found to be genetically closely related (allelic distance < 20), which suggests an epidemiological link. Thirty-seven virulence genes (VGs) were identified among 87 V. cholerae non-O1/non-O139 isolates, which resulted in 38 virulence profiles (VPs). VPs were similar between clinical and environmental isolates, with the exception of one clinical isolate that displayed a higher abundance of VGs. Also, a cluster of 11 environmental isolates was identified to have the lowest number of VGs. Among all strains, the predominant virulence factors were quorum sensing protein (luxS), repeats-in-toxins (rtxC/rtxD), hemolysin (hlyA) and different type VI secretion systems (T6SS) genes. The genotypic profiles revealed antibiotic resistance genes (ARGs) associated with resistance to beta-lactams, quinolones, macrolides, tetracycline, antifolate, aminoglycosides, fosfomycin, phenicols and sulfonamide. Carbapenemase gene VCC-1 was detected in 10 meropenem-resistant V. cholerae non-O1/non-O139 isolates derived from surface water in Germany. The proportion of resistance among V. cholerae non-O1/non-O139 species isolates against first line treatment (3rd generation cephalosporin, tetracycline and fluoroquinolone) was low. Empirical treatment would likely have been effective for all of the clinical V. cholerae non-O1/non-O139 isolates examined. Nevertheless, carbapenem-resistant isolates have been present in fresh water in Germany and might represent a reservoir for ARGs. Monitoring antimicrobial resistance is crucial for public health authorities to minimize the risks for the human population.

show abstract

Genetic approach toward linkage of Iran 2012–2016 cholera outbreaks with 7th pandemic Vibrio cholerae

Jalalizadeh,

Njamkepo,

Weill

et al. 2024

BMC Microbiol

View full text Add to dashboard Cite

Vibrio cholerae, as a natural inhabitant of the marine environment is among the world-leading causes of diarrheal diseases. The present study aimed to investigate the genetic relatedness of Iran 2012–2016 V. cholerae outbreaks with 7th pandemic cholera and to further characterize the non-ST69/non-ST75 sequence types strains by whole-genome sequencing (WGS).Twenty V. cholerae isolates related to 2012, 2013, 2015 and 2016 cholera outbreaks were studied by two genotyping methods – Pulsed-field Gel Electrophoresis (PFGE) and Multi-locus Sequence Typing (MLST)–and by antimicrobial susceptibility testing. Seven sequence types (STs) and sixteen pulsotypes were detected. Sequence type 69 was the most abundant ST confirming that most (65%, 13/20) of the studied isolates collected in Iran between 2012 and 2016 belonged to the 7th pandemic clone. All these ST69 isolates (except two) exhibited similar pulsotypes. ST75 was the second most abundant ST. It was identified in 2015 and 2016. ST438, ST178, ST579 and STs of 983 and 984 (as newfound STs) each were only detected in one isolate. All strains collected in 2016 appeared as distinct STs and pulsotypes indicative of probable different originations. All ST69 strains were resistant to nalidixic acid. Moreover, resistance to nalidixic acid, trimethoprim-sulfamethoxazole and tetracycline was only observed in strains of ST69. These properties propose the ST69 as a unique genotype derived from a separate lineage with distinct resistance properties. The circulation of V. cholerae ST69 and its traits in recent years in Iran proposes the 7th pandemic strains as the ongoing causes of cholera outbreaks in this country, although the role of ST75 as the probable upcoming dominant ST should not be ignored.Genomic analysis of non-ST69/non-ST75 strains in this study showed ST579 is the most similar ST type to 7th pandemic sequence types, due to the presence of wild type-El Tor sequences of tcpA and VC-1319, VC-1320, VC-1577, VC-1578 genes (responsible for polymyxin resistance in El Tor biotype), the traits of rstC of RS1 phage in one strain of this ST type and the presence of VPI-1 and VSP-I islands in ST579 and ST178 strains. In silico analysis showed no significant presence of resistance genes/cassettes/plasmids within non-ST69/non-ST75 strains genomes. Overall, these data indicate the higher susceptibility of V. cholerae non-ST69/non-ST75 strains in comparison with more ubiquitous and more circulating ST69 and ST75 strains.In conclusion, the occurrence of small outbreaks and sporadic cholera cases due to V. cholerae ST69 in recent years in Iran shows the 7th pandemic strains as the persistent causes of cholera outbreaks in this country, although the role of ST75 as the second most contributed ST should not be ignored. The occurrence of non-ST69/non-ST75 sequence types with some virulence factors characteristics in border provinces in recent years is noteworthy, and further studies together with surveillance efforts are expected to determine their likely route of transport.

show abstract

Analysis of the antimicrobial resistance gene frequency in whole-genome sequenced Vibrio from Latin American countries

Cited by 3 publications

References 28 publications

First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil

First Report of Vibrio vulnificus Outbreak in Farm-Raised Sorubim (Pseudoplatystoma sp.) from Brazil

Virulence and resistance patterns of Vibrio cholerae non-O1/non-O139 acquired in Germany and other European countries

Genetic approach toward linkage of Iran 2012–2016 cholera outbreaks with 7th pandemic Vibrio cholerae

Contact Info

Product

Resources

About