Analysis of the 16S rRNA gene of Anaplasma platys detected in dogs from Brazil

Cardozo, G.P.; Oliveira, L.P.; Zissou, V.G.; Donini, I.A.N.; Roberto, Patrícia G.; Marins, Mozart

doi:10.1590/s1517-83822007000300017

Cited by 9 publications

(9 citation statements)

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“…Desta forma, o protocolo para reação de nPCR, avaliado neste trabalho, mostrou-se eϐicaz e especíϐico para o diagnóstico de A.platys. Cardozo et al 2007 analisaram o gene 16S rRNA da A. Platys detectado pela nPCR e descreveram a importância do seqüenciamento do DNA para desenvolvimento da especiϐicidade e sensibilidade dos exames moleculares e para melhor caracterizar as linhagens circulantes no Brasil. A incidência de A. platys no Brasil é baixa (Macieira et al 2005) e a caracterização molecular envolvendo a comparação de seqüências de DNA de cepas brasileiras com cepas ao redor do mundo é muito importante, porém, neste estudo não foi possível a realização do seqüenciamento das amostras positivas.…”

Section: Methodsunclassified

Investigação molecular de Ehrlichia spp. e Anaplasma platys em felinos domésticos: alterações clínicas, hematológicas e bioquímicas

et al. 2011

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ABSTRACT.-Corrêa E.S., Paludo G.R., Scalon M.C., Machado J.A., Lima A.C.Q., Pinto A. Ehrlichia sp. and Anaplasma platys are Gram-negative micro-organisms, obligate intracellular parasites, residing in cytoplasmic vacuoles of leukocytes and platelets, found in peripheral blood or tissue. Few reports have been made about ehrlichiosis and anaplasmosis in cats in Brazil, which are based on the presence of morulae in leukocytes and platelets, or by detecting antibodies. The objective of this study was to investigate the natural infection with Ehrlichia sp. and A. platys in cats in Campos dos Goytacazes-RJ, by hematoscopia and DNA detection of these agents. Samples of whole blood and serum from 91 cats, regardless of race, gender and age. Blood count, serum biochemistry and PCR using primers for Ehrlichia sp. and A. platys were perfomed. Data from hematoscopia showed 9.89% of morulae only in platelets. The DNA of A. platys was detected in 13.18% of the 91 samples and 44.44% of the positive at hematoscopia. The DNA of Ehrlichia sp. was not detected in any sample. All animals studied did not show clinical signs neither positive laboratory results. The data suggest that domestic cats can serve as potential reservoirs for A. platys as asymptomatic form of related diseases.

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Section: Methodsunclassified

Investigação molecular de Ehrlichia spp. e Anaplasma platys em felinos domésticos: alterações clínicas, hematológicas e bioquímicas

et al. 2011

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“…Therefore, many clinicians tend to use combinations of antibiotics for treatment of the two parasites. Indeed, in the region of Ribeirão Preto we found thrombocytopenic dogs co-infected with E. canis and Babesia sp or Anaplasma platys , a Gram-negative bacterium which multiplies inside dog platelets and causes canine infectious cyclic thrombocytopenia (4,11). This fact emphasizes the need for more precise exams to permit a correct diagnosis by veterinary clinicians.…”

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“…The occurrence of other species of tick-transmitted pathogens might be too low to be detected in the tick or in the vertebrate host in which clinical signs may not manifest. One example is the detection of A. platys DNA in dogs (4). This parasite is also believed to be transmitted by R. sanguineus , although there are no molecular data to confirm it.…”

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Molecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil

Oliveira¹,

Cardozo²,

Santos³

et al. 2009

Braz. J. Microbiol.

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The partial DNA sequences of the 18S rRNA gene of Babesia canis and the 16S rRNA gene of Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil, were compared to sequences from other strains deposited in GenBank. The E. canis strain circulating in Ribeirão Preto is identical to other strains previously detected in the region, whereas the subspecies Babesia canis vogeli is the main Babesia strain circulating in dogs from Ribeirão Preto.

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“…It is freely available for noncommercial use and can be downloaded from http://www.heranza.com.br/bioinformatica2.htm. As a portable program ran under Windows, Linux or Mac, SNUFER is a useful tool for the localization and presentation of SNPs detected, for example, in genes used for phylogenetic studies [5,6]. …”

Section: Discussionmentioning

confidence: 99%

SNUFER: A software for localization and presentation of single nucleotide polymorphisms using a Clustal multiple sequence alignment output file

Cardozo¹,

Santos²,

Marins³

2008

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Abstract:SNUFER is software for the automatic localization and generation of tables used for the presentation of single nucleotide polymorphisms (SNPs). A multiple sequence alignment is generated using ClustalW ran inside SNUFER using an input of a FASTA file containing the sequences to be analyzed. The ClustalW output file is then used to generate a table which displays the SNPs detected in the aligned sequences and their degree of similarity. This table can be exported to Microsoft Word, Microsoft Excel or as a single text file, permitting further editing for publication. The software was written using Delphi 7 for programming and FireBird 2.0 for sequence database management. It is freely available for non-commercial use and can be downloaded from the URL http://www.heranza.com.br/bioinformatica2.htm.Keywords: software; single nucleotide polymorphisms; multiple sequence alignment; ClustalW Background:In pathogenic microorganisms, single nucleotide polymorphisms (SNP) are used to define strains and are analyzed for possible correlations with geographic origin and differences in pathogenesis [1, 2]. After DNA sequencing of gene fragments from different individuals of the same species, SNPs are identified by multiple alignment of DNA sequences. One of the most popular software used for this purpose is Clustal, which employs a fast pairwise alignment algorithm [3], helping the user to identify identical and non-identical nucleotides along sequences. The most popular way of presenting SNPs based on these results is to manually build a table which shows the nucleotide changes between a selected reference sequence and the other aligned sequences. SNUFER was developed to automatize this task by integrating the steps of multiple sequence alignment, SNP identification and data presentation in a publication format.

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Analysis of the 16S rRNA gene of Anaplasma platys detected in dogs from Brazil

Abstract: Comparison of the partial DNA sequence of the 16S rRNA gene of Anaplasma platys detected in dogs from Ribeirão Preto, Brazil, to sequences of other strains previously deposited in GenBank showed that there are at least three A. platys strains circulating in South America.

Cited by 9 publications

References 7 publications

Investigação molecular de Ehrlichia spp. e Anaplasma platys em felinos domésticos: alterações clínicas, hematológicas e bioquímicas

Investigação molecular de Ehrlichia spp. e Anaplasma platys em felinos domésticos: alterações clínicas, hematológicas e bioquímicas

Molecular analysis of the rRNA genes of Babesia spp and Ehrlichia canis detected in dogs from Ribeirão Preto, Brazil

SNUFER: A software for localization and presentation of single nucleotide polymorphisms using a Clustal multiple sequence alignment output file

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