Analysis of testosterone-hydroxylated metabolites in human urine by ultra high performance liquid chromatography-Mass Spectrometry

Escobar-Wilches, Derly Constanza; Ventura‐Bahena, Arianna; Torres‐Sánchez, Luisa; Figueroa, Mario; Sierra-Santoyo, Adolfo

doi:10.1016/j.ab.2020.113670

Cited by 6 publications

(4 citation statements)

References 41 publications

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“…A fragment ion at m/z 317.0665 originated from the ion at m/z 479.1180; it was related to hexose loss and it was identified as isorhamnetin-3-O-glucoside using previously reported data [35]. Peak ) and was tentatively identified as 16α-hydroxyestrone using previously reported data [37]. The mass spectrum of peak 14 revealed an ion at m/z 466.2669.…”

Section: Uhplc-ms Analysismentioning

confidence: 82%

“…Peak 12 appeared at m / z 306.2071 [M + NH 4 ] + and it was identified as estra-1,3,5(10)-triene-3,11,17-triol based on the typical fragment ions at m / z 107.0490 ([M + H − C 11 H 18 O 2 ] + ) [ 36 ]. Peak 13 ( m / z 304.1915 [M + NH 4 ] + ) had an MS 2 ion at m / z 112.1111 ([C 6 H 8 O 2 ] + ) and was tentatively identified as 16 α -hydroxyestrone using previously reported data [ 37 ]. The mass spectrum of peak 14 revealed an ion at m / z 466.2669.…”

Section: Resultsmentioning

confidence: 99%

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Phytochemical Analysis, Antioxidant and Enzyme-Inhibitory Activities, and Multivariate Analysis of Insect Gall Extracts of Picea koraiensis Nakai

Wang

Sun

et al. 2023

Molecules

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Picea koraiensis Nakai (PK) is an evergreen tree. It plays an important role in landscaping and road greening. Insect galls of PK are formed by parasitism of the adelgid Adelges laricis. Except for phenolics, other chemical constituents and biological activity of insect gall from PK are still unknown. Thus, here, we performed phytochemical and biological activity analyses of PK insect gall extracts, aiming to turn waste into treasure and serve human health. PK insect gall extracts were prepared using seven solvents. Antioxidant activities of the extracts were examined via antioxidant assays (radical and oxidizing substance quenching, metal chelating, and reducing power). The inhibitory activities of the extracts were determined toward the key human-disease-related enzymes α-glucosidase, α-amylase, cholinesterase, tyrosinase, urease, and xanthine oxidase. The content of numerous active constituents was high in the methanol and ethanol extracts of PK insect gall, and these extracts had the highest antioxidant and enzyme-inhibitory activities. They also showed excellent stability and low toxicity. These extracts have potential for use as stabilizers of olive and sunflower seed oils.

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Section: Uhplc-ms Analysismentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Phytochemical Analysis, Antioxidant and Enzyme-Inhibitory Activities, and Multivariate Analysis of Insect Gall Extracts of Picea koraiensis Nakai

Wang

Sun

et al. 2023

Molecules

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“…In addition to chemical depletion, the unretained fraction of sample preparation constitutes a simplified alternative to charcoal stripping. For example, Escobar-Wilches and collegues collected the SPE wash step to obtain a steroid-free urine [108]. Because these surrogate matrices do not fully represent the original matrix, the use of an IS is essential to correct for the recovery yield [109].…”

Section: Authentic Analyte(s) In Surrogate Matrixmentioning

confidence: 99%

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

Visconti

Bernard

Feinberg

et al. 2023

Analytica Chimica Acta

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“…Considering a wide list of potential analytes, nowadays, the most common sample preparation techniques are dilute-and-shoot, liquid-liquid extraction (LLE) [23][24][25], dispersive liquid-liquid microextraction (DLLME) [26][27][28][29], as well as solid-phase extraction (SPE) [30][31][32] coupled with mineral or enzymatic hydrolysis. As a rule, hydrolysis is applied in the case of urinalysis, since steroid hormones are mostly present in the conjugated form (as glucuronides or sulfates) in this matrix and cannot be detected directly by GC-MS(/MS).…”

Section: Introductionmentioning

confidence: 99%

GC-MS/MS Determination of Steroid Hormones in Urine Using Solid-Phase Derivatization as an Alternative to Conventional Methods

et al. 2022

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Solid-phase analytical derivatization (SPAD) is a promising hybrid sample preparation technique combining the clean-up and preconcentration of the sample in a single step. In this work, a novel SPAD method based on the preparation of trimethylsilyl (TMS) derivatives of steroid hormones (testosterone, estrone, DHT, estriol, estradiol, and progesterone) in Phenomenex Strata C18-E (100 mg, 1 mL) cartridges has been developed and applied for their GC-MS/MS determination in human urine samples. The proposed procedure allows the detection and quantification of steroids with limits of 1.0–2.5 and 2.5–5 ng/mL, respectively. These characteristics are comparable with those obtained with a conventional liquid–liquid extraction, while the recovery of analytes in the proposed SPAD procedure is higher. The major advantages of SPAD are a short derivatization time, high efficiency, and the possibility to automatize the procedure. However, its cost-effectiveness in routine practice is still questionable.

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Analysis of testosterone-hydroxylated metabolites in human urine by ultra high performance liquid chromatography-Mass Spectrometry

Cited by 6 publications

References 41 publications

Phytochemical Analysis, Antioxidant and Enzyme-Inhibitory Activities, and Multivariate Analysis of Insect Gall Extracts of Picea koraiensis Nakai

Phytochemical Analysis, Antioxidant and Enzyme-Inhibitory Activities, and Multivariate Analysis of Insect Gall Extracts of Picea koraiensis Nakai

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

GC-MS/MS Determination of Steroid Hormones in Urine Using Solid-Phase Derivatization as an Alternative to Conventional Methods

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