Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages

Mian, MA Rouf; Zhang, Yan; Wang, Zeng‐Yu; Zhang, Jiyi; Cheng, Xiaofei; Chen, Lei; Chekhovskiy, Konstantin; Dai, Xinbin; Mao, Chunhong; Cheung, Foo; Zhao, Xuefeng; He, Ji; Scott, Angela D.; Town, Christopher D.; May, Gregory D.

doi:10.1186/1471-2229-8-27

Cited by 24 publications

(18 citation statements)

References 46 publications

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“…Plants respond to high temperature by synthesizing a set of the heat shock proteins (HSPs) to acquire thermotolerance [ 33 ]. According to their predicted molecular weight, HSPs could be grouped into three families: high molecular weight (HMW-HSP, 80–114 kDa), HSP70 (69–71 kDa), low molecular weight (LMW-HSP, 15–30 kDa) [ 13 ]. Many researches have proved that the increased transcript abundance of the three kinds of HSPs and their molecular chaperone was directly involved in the activation of protection mechanisms in response to heat stress [ 34 – 37 ].…”

Section: Resultsmentioning

confidence: 99%

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An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance

Sun

Zhang

et al. 2014

BMC Genomics

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BackgroundTall fescue (Festuca arundinacea Schreb.) is major cool-season forage and turf grass species worldwide, but high-temperature is a major environmental stress that dramatically threaten forage production and turf management of tall fescue. However, very little is known about the whole-genome molecular mechanisms contributing to thermotolerance. The objectives of this study were to analyzed genome-wide gene expression profiles in the leaves of two tall fescue genotypes, heat tolerant ‘PI578718’ and heat sensitive ‘PI234881’ using high-throughput RNA sequencing.ResultsA total of 262 million high-quality paired-end reads were generated and assembled into 31,803 unigenes with an average length of 1,840 bp. Of these, 12,974 unigenes showed different expression patterns in response to heat stress and were categorized into 49 Gene Ontology functional subcategories. In addition, the variance of enrichment degree in each functional subcategory between PI578718 and PI234881 increased with increasing treatment time. Cell division and cell cycle genes showed a massive increase in transcript abundance in heat-stressed plants and more activated genes were detected in PI 578718 by Kyoto Encyclopedia of Genes and Genomes pathways analysis. Low molecular weight heat shock protein (LMW-HSP, HSP20) showed activated in two stressed genotypes and high molecular weight HSP (HMW-HSP, HSP90) just in PI578718. Assimilation such as photosynthesis, carbon fixation, CH4, N, S metabolism decreased along with increased dissimilation such as oxidative phosphorylation.ConclusionsThe assembled transcriptome of tall fescue could serve as a global description of expressed genes and provide more molecular resources for future functional characterization analysis of genomics in cool-season turfgrass in response to high-temperature. Increased cell division, LMW/HMW-HSP, dissimilation and antioxidant transcript amounts in tall fescue were correlated with successful resistance to high temperature stress.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-1147) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…The genome size of tall fescue is approximately 6 × 10 3 Mbp and about 14 times larger than that of rice ( Oryza sativa L.) [ 13 ]. To date, complete genome sequences of tall fescue are not achieved.…”

Section: Introductionmentioning

confidence: 99%

An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance

Sun

Zhang

et al. 2014

BMC Genomics

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“…Available genomic resources for the Lolium/Schedonorus/ Festuca grasses, and their endophytes, are quite meagre compared with most agronomic crops (Mian et al, 2008;Dinkins et al, 2012;Studer et al, 2012;Farrell et al, 2014;Hu et al, 2014;Dupont et al, 2015). The large genome size of hexaploid tall fescue (c. 6 Gb) makes conventional genome sequencing studies impractical (Seal, 1983), although a first draft genome sequence of a related diploid, Lolium perenne, has recently been released (Byrne et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome response ofLolium arundinaceumto its fungal endophyteEpichloë coenophiala

et al. 2016

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SummaryTall fescue (Lolium arundinaceum) is one of the primary forage and turf grasses in temperate regions of the world. A number of favourable characteristics of tall fescue are enhanced by its seed-transmissible fungal symbiont (endophyte) Epichlo€ e coenophiala.Our approach was to assemble the tall fescue transcriptome, then identify differentially expressed genes (DEGs) for endophyte-symbiotic (E+) vs endophyte-free (EÀ) clones in leaf blades, pseudostems, crowns and roots. RNA-seq reads were used to construct a tall fescue reference transcriptome and compare gene expression profiles.Over all tissues examined, 478 DEGs were identified between the E+ and EÀ clones for at least one tissue (more than two-fold; P < 0.0001, 238 E+ > EÀ and 240 EÀ > E+), although no genes were differentially expressed in all four tissues. Gene ontology (GO) terms, GO:0010200 (response to chitin), GO:0002679 (respiratory burst during defence response) and GO:0035556 (intracellular signal transduction) were significantly overrepresented among 25 EÀ > E+ DEGs in leaf blade, and a number of other DEGs were associated with defence and abiotic response.In particular, endophyte effects on various WRKY transcription factors may have implications for symbiotic stability, endophyte distribution in the plant, or defence against pathogens.

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“…Grasslands occupy about twice the land area of grain crops throughout the world ( Mian et al, 2008 ) and play important ecological roles in the biosphere. For example, grasslands influence the carbon cycle, as well as the nitrogen cycle.…”

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid Alleviates Damage from Heat Stress in the Photosystem II of Tall Fescue in Both the Photochemical and Thermal Phases

et al. 2017

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L-Ascorbate (Asc) plays important roles in plant development, hormone signaling, the cell cycle and cellular redox system, etc. The higher content of Asc in plant chloroplasts indicates its important role in the photosystem. The objective of this study was to study the roles of Asc in tall fescue leaves against heat stress. After a heat stress treatment, we observed a lower value of the maximum quantum yield for primary photochemistry (φPo), which reflects the inhibited activity of the photochemical phase of photosystem II (PSII). Moreover, we observed a higher value of efficiency of electron transfer from QB to photosystem I acceptors (δR0), which reflects elevated activity of the thermal phase of the photosystem of the tall fescue. The addition of Asc facilitate the behavior of the photochemical phase of the PSII by lowering the ROS content as well as that of the alternative electron donor to provide electron to the tyrosine residue of the D1 protein. Additionally, exogenous Asc reduces the activity of the thermal phase of the photosystem, which could contribute to the limitation of energy input into the photosystem in tall fescue against heat stress. Synthesis of the Asc increased under heat stress treatment. However, under heat stress this regulation does not occur at the transcription level and requires further study.

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Analysis of tall fescue ESTs representing different abiotic stresses, tissue types and developmental stages

Cited by 24 publications

References 46 publications

An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance

An RNA sequencing transcriptome analysis of the high-temperature stressed tall fescue reveals novel insights into plant thermotolerance

Transcriptome response ofLolium arundinaceumto its fungal endophyteEpichloë coenophiala

Ascorbic Acid Alleviates Damage from Heat Stress in the Photosystem II of Tall Fescue in Both the Photochemical and Thermal Phases

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