Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

Duceppe, Marc‐Olivier; Lafond-Lapalme, Joël; Palomares-Rius, Juan E.; Sabeh, Michaël; Blok, Vivian C.; Moffett, Peter; Mimee, Benjamin

doi:10.1038/s41598-017-03871-x

Cited by 20 publications

(30 citation statements)

References 72 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A Globodera neprilysin gene has been detected as the first transcript to be upregulated in eggs treated with host root exudate ( Duceppe et al, 2017 ), therefore we tested the expression of Gpa-nep-1 in G. pallida eggs that had been incubated in non-infested control and aphid-infested PRE. There was a significant increase in the expression of Gpa-nep-1 in unhatched G. pallida eggs 8 days post incubation in root exudates from non-infested control plants relative to eggs incubated in water ( Figure 6A ).…”

Section: Resultsmentioning

confidence: 99%

“…The effect of sugars on hatching also correlated with an increase in Gpa-nep-1 transcript within the eggs. In a previous study, a role in hatching has been proposed for this gene as it is the first Globodera transcript to be upregulated post-treatment with root exudates from host plants ( Duceppe et al, 2017 ). This study reinforces that proposed link as it correlates the hatching ability of the exudate with expression levels of the gene.…”

Section: Discussionmentioning

confidence: 99%

“…The compounds required for nematode hatch and the mechanisms behind eclosion remain poorly characterized. Additionally, the majority of genes involved in the hatching response has not been uncovered, however, a G. rostochiensis neprilysin gene ( Gro-nep-1 ) was identified as the first transcript to be upregulated in eggs treated with host root exudate ( Duceppe et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aphid Colonization Affects Potato Root Exudate Composition and the Hatching of a Soil Borne Pathogen

et al. 2018

View full text Add to dashboard Cite

Plants suffer multiple, simultaneous biotic threats from both above and below ground. These pests and/or pathogens are commonly studied on an individual basis and the effects of above-ground pests on below-ground pathogens are poorly defined. Root exudates from potato plants (Solanum tuberosum L.) were analyzed to characterize the top-down plant-mediated interactions between a phloem-sucking herbivore (Myzus persicae) and a sedentary, endoparasitic nematode (Globodera pallida). Increasing inocula of the aphid, M. persicae, reduced the root mass of potato plants. Exudates collected from these roots induced significantly lower hatching of second-stage juveniles from G. pallida eggs over a 28-day period, than those from uninfested control plants. Inhibition of hatch was significantly positively correlated with size of aphid inoculum. Diminished hatching was partially recovered after treatment with root exudate from uninfested potato plants indicating that the effect on hatching is reversible but cannot be fully recovered. Glucose and fructose content was reduced in root exudates from aphid-infested potato plants compared to controls and these sugars were found to induce hatching of G. pallida, but not to the same degree as potato root exudates (PRE). Supplementing aphid-infested PRE with sugars did not recover the hatching potential of the treatment, suggesting that additional compounds play an important role in egg hatch. The first gene upregulated in the closely related potato cyst nematode Globodera rostochiensis post-exposure to host root exudate, Neprilysin-1, was confirmed to be upregulated in G. pallida cysts after exposure to PRE and was also upregulated by the sugar treatments. Significantly reduced upregulation of Gpa-nep-1 was observed in cysts treated with root exudates from potato plants infested with greater numbers of aphids. Our data suggest that aphid infestation of potato plants affects the composition of root exudates, with consequential effects on the hatching and gene expression of G. pallida eggs. This work shows that an above-ground pest can indirectly impact the rhizosphere and reveals secondary effects for control of an economically important below-ground pathogen.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Aphid Colonization Affects Potato Root Exudate Composition and the Hatching of a Soil Borne Pathogen

et al. 2018

View full text Add to dashboard Cite

show abstract

“…This study took advantage of a recently published transcriptome dataset from a G . rostochiensis hatching experiment conducted by our team [ 18 ]. These data (NCBI bioproject accession number PRJNA274143) include sampling at different life stages, including dry cyst, hydrated cyst, hydrated cyst soaked in PRD for 1 h, 8 h, 24 h, 48 h, and 7 d, and fully hatched infective larvae (J2).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (Globodera spp.)

et al. 2018

Self Cite

View full text Add to dashboard Cite

Relative gene expression analyses by qRT-PCR (quantitative reverse transcription PCR) require an internal control to normalize the expression data of genes of interest and eliminate the unwanted variation introduced by sample preparation. A perfect reference gene should have a constant expression level under all the experimental conditions. However, the same few housekeeping genes selected from the literature or successfully used in previous unrelated experiments are often routinely used in new conditions without proper validation of their stability across treatments. The advent of RNA-Seq and the availability of public datasets for numerous organisms are opening the way to finding better reference genes for expression studies. Globodera rostochiensis is a plant-parasitic nematode that is particularly yield-limiting for potato. The aim of our study was to identify a reliable set of reference genes to study G. rostochiensis gene expression. Gene expression levels from an RNA-Seq database were used to identify putative reference genes and were validated with qRT-PCR analysis. Three genes, GR, PMP-3, and aaRS, were found to be very stable within the experimental conditions of this study and are proposed as reference genes for future work.

show abstract

“…This enzyme is known to activate/inactivate peptide hormones and may be involved in a cascade of events leading to hatching. After 48 h of exudate exposure, G. rostochiensis had 278 differentially expressed genes, several of which are known effector genes (Duceppe et al, 2017).…”

Section: Modes Of Action Of Root Metabolites In Nematodesmentioning

confidence: 99%

Impacts of Root Metabolites on Soil Nematodes

Sikder

Vestergård

2020

Front. Plant Sci.

View full text Add to dashboard Cite

Plant parasitic nematodes cause significant crop damage globally. Currently, many nematicides have been banned or are being phased out in Europe and other parts of the world because of environmental and human health concerns. Therefore, we need to focus on sustainable and alternative methods of nematode control to protect crops. Plant roots contain and release a wide range of bioactive secondary metabolites, many of which are known defense compounds. Hence, profound understanding of the root mediated interactions between plants and plant parasitic nematodes may contribute to efficient control and management of pest nematodes. In this review, we have compiled literature that documents effects of root metabolites on plant parasitic nematodes. These chemical compounds act as either nematode attractants, repellents, hatching stimulants or inhibitors. We have summarized the few studies that describe how root metabolites regulate the expression of nematode genes. As non-herbivorous nematodes contribute to decomposition, nutrient mineralization, microbial community structuring and control of herbivorous insect larvae, we also review the impact of plant metabolites on these nontarget organisms.1 study on effect of both plant and specific metabolites detected in root exudates of the plant, 2 study on effect of plant, 3 study on effect of synthetic compound on nematodes, NA indicate not applicable.

show abstract

Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

Cited by 20 publications

References 72 publications

Aphid Colonization Affects Potato Root Exudate Composition and the Hatching of a Soil Borne Pathogen

Aphid Colonization Affects Potato Root Exudate Composition and the Hatching of a Soil Borne Pathogen

Transcriptome-wide selection of a reliable set of reference genes for gene expression studies in potato cyst nematodes (Globodera spp.)

Impacts of Root Metabolites on Soil Nematodes

Contact Info

Product

Resources

About