Analysis of sucrose-induced small RNAs in Streptococcus mutans in the presence of different sucrose concentrations

Liu, Shan Shan; Zhu, Wen Hui; Zhi, Qinghui; Liu, Jia; Yan, Wang; Lin, Huancai

doi:10.1007/s00253-017-8346-x

Cited by 13 publications

(12 citation statements)

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“…Initially, a range of concentrations were evaluated (0.125, 0.25, 0.5, 1, 2, and 5% w/v) in terms of biomass and pH, with the resultant biofilm data demonstrating that the carbohydrate source, irrespective of concentration, showed a consistent trend ( Supplementary Figure S2 ), i.e., sucrose consistently gave greater biomass and lower pH, whereas galactose displayed the contrasting polarized effects. We therefore focused on the concentration of 1% w/v with caries-free ( n = 10) and caries ( n = 10) isolates, a concentration which has been a consistently reported within analogous studies ( Zeng et al, 2013 ; Liu S.S. et al, 2017 ; Cai et al, 2018 ). These data showed that although sucrose displayed the greatest biomass and lowest pH, followed by mannose, glucose, fructose and galactose, there were no significant differences between these caries groups in terms of biomass and pH ( Figures 1A,B ).…”

Section: Resultsmentioning

confidence: 99%

“…Significant differences were observed, and were not only dependent on the sugars, but also on specific isolates. Liu S.S. et al (2017) revealed S. mutans isolates varied in their ability to compete and become dominant in the biofilm after the addition of sucrose ( Liu Y. et al, 2017 ). Interactions between microorganisms colonizing the oral cavity are consecutive factors affecting the development of biofilm, with interactions occurring between microorganisms resulting in both the acceleration and inhibition of this process ( Wen et al, 2010 ; Redanz et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

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Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community

et al. 2018

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Aim: The purpose of this study was to investigate strain dependent differences of the cariogenic biofilm forming Streptococcus mutans within both simple and complex communities.Methods: A mono-species containing representative S. mutans clinical isolates (caries and non-caries), and a multispecies in vitro caries biofilm model containing Lactobacillus casei, Veillonella dispar, Fusobacterium nucleatum and Actinomyces naeslundii, and either of two representative S. mutans clinical isolates (caries and non-caries), was developed as a comparison model. Compositional analysis of total and live bacteria within biofilms, and transcriptional analysis of biofilm associated virulence factors were evaluated by live/dead PCR and quantitative PCR, respectively. Scanning electron microscopy (SEM) was used to analyze the architecture of biofilm. One-way analysis of variance and t-tests were used to investigate significant differences between independent groups of data.Results: Within a mono-species biofilm, different S. mutans strains responded similarly to one another during biofilm formation in different carbohydrate sources, with sucrose showing the highest levels of biofilm biomass and galactose showing the lowest. Within the polymicrobial biofilm system, compositional analysis of the bacteria within the biofilm showed that S. mutans derived from a caries-free patient was preferentially composed of both total and viable L. casei, whereas S. mutans derived from a caries patient was dominated by both total and viable S. mutans (p < 0.001). Normalized gene expression analysis of srtA, gtfB, ftf, spaP, gbpB, and luxS, showed a general upregulation within the S. mutans dominant biofilm.Conclusion: We were able to demonstrate that individual strains derived from different patients exhibited altered biofilm characteristics, which were not obvious within a simple mono-species biofilm model. Influencing the environmental conditions changed the composition and functionality S. mutans within the polymicrobial biofilm. The biofilm model described herein provides a novel and reproducible method of assessing the impact on the biofilm microbiome upon different environmental influences.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community

et al. 2018

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“…Importantly, sRNAs coordinate adaptation processes in response to environmental changes in bacteria by regulating target mRNA expression [37][38][39][40][41]. Specifically, srn225147 and srn821798 were sequenced in our previous studies [22,23]. Virulence-related genes selected in our present study were predicted targets for these two sRNAs according to IntaRNA and RNAPredator.…”

Section: Discussionmentioning

confidence: 92%

“…The PCR reaction conditions for srn821798 were as follows: denaturation at 95°C for 10 s; 40 cycles consisting of 5 s of denaturation at 95°C; and 20 s of annealing at 50°C. The primers for 16S rRNA, gtfB, gtfC, spaP, srtA, ffh, and relA were synthesizedaccording to previous studies[8,[22][23][24].…”

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confidence: 99%

Phenotypes of Streptococcus mutans isolates in sound and cavitated tooth surfaces and its relationship to early childhood caries in China

Liu

Guo

et al. 2019

Preprint

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Background To compare the phenotypes of Streptococcus mutans (S. mutans ) strains isolated from sites with different caries susceptibility of a single subject. Methods This study was carried out in children with early-childhood caries (ECC) and caries-free (CF). The ECC subjects presented two sites: a cavitated lesion (ECC-C) and a sound surface (ECC-F). The CF subjects presented one sound surface. The following phenotypes were evaluated among these three sites: growth pattern, biofilm, exopolysaccharide (EPS), pH drop, and the expression levels of genes (gtfB, gtfC, spaP, srtA, relA, ffh, srn225147, and srn821798). Results All of these phenotypes were detected similarly between ECC-C and ECC-F groups. However, the capacity of biofilm formation, EPS, pH drop and the expression levels of genes (gtfB and spaP) of S. mutans in the CF group were lower compared to those of the ECC group. There was a relatively higher expression of srn821798 compared to that of srn225147 in clinical isolates, and isolates with low expression of srn821798 had lower expression levels of predicted targets (gtfB, gtfC, and spaP). Conclusions These data indicate S. mutans isolated from sites with different caries susceptibility of a single subject showed similar characteristics, while the cariogenic potential of the S. mutans isolated from ECC individuals was relatively higher than that of CF subjects.

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“…surface of a tooth, forming a biofilm and releasing acidic compounds following carbohydrate metabolism, thereby corroding the tooth surface (5). There is strong epidemiological evidence that S. mutans is more commonly detected in the oral cavity of children affected by dental caries compared to that in children free of such caries (6,7), and the forms of S. mutans isolated from children with caries have been found to be more virulent than those isolated from caries-free children (8).…”

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confidence: 99%

A Core Genome Multilocus Sequence Typing Scheme for Streptococcus mutans

Liu

Guo

et al. 2020

mSphere

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ABSTRACT Streptococcus mutans is one of the primary pathogens responsible for the development of dental caries. Recent whole-genome sequencing (WGS)-based core genome multilocus sequence typing (cgMLST) approaches have been employed in epidemiological studies of specific human pathogens. However, this approach has not been reported in studies of S. mutans. Here, we therefore developed a cgMLST scheme for S. mutans. We surveyed 199 available S. mutans genomes as a means of identifying cgMLST targets, developing a scheme that incorporated 594 targets from the S. mutans UA159 reference genome. Sixty-eight sequence types (STs) were identified in this cgMLST scheme (cgSTs) in 80 S. mutans isolates from 40 children that were sequenced in this study, compared to 35 STs identified by multilocus sequence typing (MLST). Fifty-six cgSTs (82.35%) were associated with a single isolate based on our cgMLST scheme, which is significantly higher than in the MLST scheme (11.43%). In addition, 58.06% of all MLST profiles with ≥2 isolates were further differentiated by our cgMLST scheme. Topological analyses of the maximum likelihood phylogenetic trees revealed that our cgMLST scheme was more reliable than the MLST scheme. A minimum spanning tree of 145 S. mutans isolates from 10 countries developed based upon the cgMLST scheme highlighted the diverse population structure of S. mutans. This cgMLST scheme thus offers a new molecular typing method suitable for evaluating the epidemiological distribution of this pathogen and has the potential to serve as a benchmark for future global studies of the epidemiological nature of dental caries. IMPORTANCE Streptococcus mutans is regarded as a major pathogen responsible for the onset of dental caries. S. mutans can transmit among people, especially within families. In this study, we established a new epidemiological approach to S. mutans classification. This approach can effectively differentiate among closely related isolates and offers superior reliability relative to that of the traditional MLST molecular typing method. As such, it has the potential to better support effective public health strategies centered around this bacterium that are aimed at preventing and treating dental caries.

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Analysis of sucrose-induced small RNAs in Streptococcus mutans in the presence of different sucrose concentrations

Cited by 13 publications

References 60 publications

Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community

Evaluating Streptococcus mutans Strain Dependent Characteristics in a Polymicrobial Biofilm Community

Phenotypes of Streptococcus mutans isolates in sound and cavitated tooth surfaces and its relationship to early childhood caries in China

A Core Genome Multilocus Sequence Typing Scheme for Streptococcus mutans

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