Analysis of stromal Cells Developed from Cord Blood CD34<sup>+</sup>Cells

Ryu, Kyung‐Ha; Park, Se Jin; Kim, Kyung Hyo; Khan, Mohammad Tahir; Seoh, Ju Young; Shin, Hee‐Young; Ahn, Hyo‐Won

doi:10.4110/in.2001.1.1.87

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“…During the in vitro culture of human CB CD34 + cells, many types of cells including stromal cells are generated (Nieda et al , 1997; Ryu et al, 2001) and it is likely that certain cytokines secreted by the stromal cells may influence the differentiation of other cell types. Therefore, the possibility that IL‐7 or IL‐2 produced by the stromal cells may have influenced the development of NKT cells cannot be excluded, as blocking experiments were not performed.…”

Section: Discussionmentioning

confidence: 99%

In vitro differentiation of natural killer T cells from human cord blood CD34⁺ cells

Woo¹,

Jung²,

Ryu³

et al. 2003

Br J Haematol

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Summary. Natural killer T (NKT) cells are involved in innate immune defence and also in the regulation of adaptive immune responses. However, the development of NKT cells in vitro has not been fully characterized and culture conditions have not been fully optimized. In the present study, we found that an NKT cell fraction developed during the in vitro culture of cord blood (CB) CD34 + cells, and this was subsequently characterized both phenotypically and morphologically. CD34 + cells purified from 10 human CB were cultured in the presence of several cytokines and analysed by flow cytometry, light microscopy and electron microscopy. The NKT cell fraction, defined phenotypically (CD3 + CD16 + CD56 + CD94 + ) as expressing the invariant T-cell receptor Va24 and Vb11, appeared in the CD56 hi fractions. Intracytoplasmic staining demonstrated that interferon-c and interleukin 4 (IL-4) were detected in the CD56 hi fractions. IL-15 was essential and, in combination with either flt3-ligand (FL) or stem cell factor (SCF), was sufficient to induce the development of NKT cells. The phenotype of the NKT cell fraction was CD45RO + CD45RA -and CD4 + CD8a + . Morphologically, they were very large, with either round or oval nuclei, moderately condensed chromatins, voluminous weakly basophilic cytoplasm and various cytoplasmic granules such as dense core granules, multivesicular bodies, and intermediate form granules. When CD34 + cells purified from bone marrow (BM) were compared with those from CB, the latter were consistently more efficient at generating CD56 hi NKT cell fractions. In conclusion, IL-15 in combination with FL and/or SCF can induce the differentiation of NKT cells from human CB CD34 + cells.

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Section: Discussionmentioning

confidence: 99%

In vitro differentiation of natural killer T cells from human cord blood CD34⁺ cells

Woo¹,

Jung²,

Ryu³

et al. 2003

Br J Haematol

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Analysis of stromal Cells Developed from Cord Blood CD34⁺Cells

Cited by 1 publication

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In vitro differentiation of natural killer T cells from human cord blood CD34⁺ cells

In vitro differentiation of natural killer T cells from human cord blood CD34⁺ cells

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Analysis of stromal Cells Developed from Cord Blood CD34+Cells

Cited by 1 publication

References 0 publications

In vitro differentiation of natural killer T cells from human cord blood CD34+ cells

In vitro differentiation of natural killer T cells from human cord blood CD34+ cells

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Analysis of stromal Cells Developed from Cord Blood CD34⁺Cells

In vitro differentiation of natural killer T cells from human cord blood CD34⁺ cells

In vitro differentiation of natural killer T cells from human cord blood CD34⁺ cells