Analysis of sterols in selected bloom-forming algae in China

Geng, Hui-Xia; Yu, Rencheng; Chen, Zhen-Fan; Peng, Qunjia; Tian, Yun; Zhou, Mingjiang

doi:10.1016/j.hal.2017.04.008

Cited by 17 publications

(7 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the settlement region of massive floating green algae in the Yellow Sea, we proposed and tested the use of 28-isofucosterol in the surface sediment as a biomarker for green algae in the genus Ulva because the green tides in the Yellow Sea are mainly formed by green alga, U. prolifera.…”

Section: Methodsmentioning

confidence: 99%

“…The extraction method of sterols from sediment samples has been listed in the previous study. 17 The final extracts were derivatized using bis-(trimethylsilyl)trifluoroacetamide (BSTFA) and trimethylchlorosilane (TMCS) (99% BSTFA + 1% TMCS), and the sterols were analyzed using gas chromatography coupled with a mass spectrometer (Agilent 7890A-GC-5975C MS, with an autosampler, USA). The separation of sterols was achieved using a silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness SPE-50 column, SUPELCO, USA).…”

Section: Sterol Biomarker Analysismentioning

confidence: 99%

“…The separation of sterols was achieved using a silica capillary column (30 m × 0.25 mm i.d., 0.25 μm film thickness SPE-50 column, SUPELCO, USA). The instrument settings and the ions for the identification and quantitative analysis of sterols under the selected ion scan mode referred to by Geng et al 17 2.4. Flow Field in the Yellow Sea Based on Numerical Modeling.…”

Section: Sterol Biomarker Analysismentioning

confidence: 99%

See 2 more Smart Citations

Green Tides in the Yellow Sea Promoted the Proliferation of Pelagophyte Aureococcus anophagefferens

Zhao

Geng

Zhang

et al. 2022

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Harmful algal blooms formed by fast-growing, ephemeral macroalgae have expanded worldwide, yet there is limited knowledge of their potential ecological consequences. Here, we select intense green tides formed by Ulva prolifera in the Yellow Sea, China, to examine the ecological consequences of these blooms. Using 28-isofucosterol in the surface sediment as a biomarker of green algae, we identified the settlement region of massive floating green algae in the area southeast of the Shandong Peninsula in the southern Yellow Sea. The responses of the phytoplankton assemblage from the deep chlorophyll-a maximum layer were then resolved using high-throughput sequencing. We found striking changes in the phytoplankton community in the settlement region after an intensive green tide in 2016, characterized by a remarkable increase in the abundance of the pelagophyte Aureococcus anophagefferens, the causative species of ecosystem disruptive brown tides. Our study strongly suggests that the occurrence of massive macroalgal blooms may promote blooms of specific groups of microalgae through alteration of the marine environment.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Sterol Biomarker Analysismentioning

confidence: 99%

Section: Sterol Biomarker Analysismentioning

confidence: 99%

See 1 more Smart Citation

Green Tides in the Yellow Sea Promoted the Proliferation of Pelagophyte Aureococcus anophagefferens

Zhao

Geng

Zhang

et al. 2022

Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Collected data were analyzed with an Agilent GC/MSD Productivity ChemStation and AMDIS (Automated Mass spectral Deconvolution and Identification System) software. Sterols were identified by comparing their retention times and mass spectra to those of sterols from dinoflagellates and other marine organisms (65)(66)(67)(68)(69)(70)(71)(72)(73)(74) and to entries in the commercial NIST/EPA/NIH Mass Spectral Library (NIST 08) GC/MS database. Sterols with similar mass spectra were identified by comparing their relative retention times with respect to cholesterol and retention indices to published values or values in the NIST database.…”

Section: Methodsmentioning

confidence: 99%

Clade-Specific Sterol Metabolites in Dinoflagellate Endosymbionts Are Associated with Coral Bleaching in Response to Environmental Cues

Jiang

Zhao

et al. 2020

mSystems

View full text Add to dashboard Cite

Cnidarians cannot synthesize sterols (which play essential roles in growth and development) de novo but often use sterols acquired from endosymbiotic dinoflagellates. While sterol availability can impact the mutualistic interaction between coral host and algal symbiont, the biosynthetic pathways (in the dinoflagellate endosymbionts) and functional roles of sterols in these symbioses are poorly understood. In this study, we found that itraconazole, which perturbs sterol metabolism by inhibiting the sterol 14-demethylase CYP51 in dinoflagellates, induces bleaching of the anemone Heteractis crispa and that bleaching perturbs sterol metabolism of the dinoflagellate. While Symbiodiniaceae have clade-specific sterol metabolites, they share features of the common sterol biosynthetic pathway but with distinct architecture and substrate specificity features of participating enzymes. Tracking sterol profiles and transcripts of enzymes involved in sterol biosynthesis across time in response to different environmental cues revealed similarities and idiosyncratic features of sterol synthesis in the endosymbiont Breviolum minutum. Exposure of algal cultures to high levels of light, heat, and acidification led to alterations in sterol synthesis, including blocks through downregulation of squalene synthase transcript levels accompanied by marked growth reductions. IMPORTANCE These results indicate that sterol metabolites in Symbiodiniaceae are clade specific, that their biosynthetic pathways share architectural and substrate specificity features with those of animals and plants, and that environmental stress-specific perturbation of sterol biosynthesis in dinoflagellates can impair a key mutualistic partnership for healthy reefs.

show abstract

“…were found to produce the rare 27-nor sterol occelasterol 68 ( Figure 4 ) [ 54 ]. It has been proposed that isofucosterol 44 is a potential biomarker for the green-tide forming multicellular alga Ulva prolifera , and that dinosterol 74 and 24 Z -propylidienecholesterol 75 are potential biomarkers for bloom-forming dinoflagellates [ 60 ] ( Figure 6 ). Toxic bloom-causing dinoflagellate Cochlodinium polykrikoides had a sterol profile including prevalent sterols of dinosterol 74 (40%), dihydrodinosterol 76 (32%), and the rare 4α-methyl sterol amphisterol 77 (23%).…”

Section: Phylogenic and Ecological Insightsmentioning

confidence: 99%

Microbial Sterolomics as a Chemical Biology Tool

Haubrich

2018

Molecules

View full text Add to dashboard Cite

Metabolomics has become a powerful tool in chemical biology. Profiling the human sterolome has resulted in the discovery of noncanonical sterols, including oxysterols and meiosis-activating sterols. They are important to immune responses and development, and have been reviewed extensively. The triterpenoid metabolite fusidic acid has developed clinical relevance, and many steroidal metabolites from microbial sources possess varying bioactivities. Beyond the prospect of pharmacognostical agents, the profiling of minor metabolites can provide insight into an organism’s biosynthesis and phylogeny, as well as inform drug discovery about infectious diseases. This review aims to highlight recent discoveries from detailed sterolomic profiling in microorganisms and their phylogenic and pharmacological implications.

show abstract

Analysis of sterols in selected bloom-forming algae in China

Cited by 17 publications

References 52 publications

Green Tides in the Yellow Sea Promoted the Proliferation of Pelagophyte Aureococcus anophagefferens

Green Tides in the Yellow Sea Promoted the Proliferation of Pelagophyte Aureococcus anophagefferens

Clade-Specific Sterol Metabolites in Dinoflagellate Endosymbionts Are Associated with Coral Bleaching in Response to Environmental Cues

Microbial Sterolomics as a Chemical Biology Tool

Contact Info

Product

Resources

About