Analysis of SSR Fingerprints in Introduced Silkworm Germplasm Resources

Construction of a primary DNA fingerprint database for cotton cultivars

“…Constructing a fingerprint of the cotton species would not only identify cotton species but also analyze their genetic distance and relationships with one another. Hou (2007) used 35 SSR markers to construct 96 silkworm race fingerprints and analyze genetic distance based on their origins. In this study, we constructed 132 DNA fingerprints of cotton species using a fluorescence-labeling detection system and analyzed the genetic diversity of the species.…”

Fingerprint analysis of Bombyx mori local variety resources based on SSR markers

“…Based on results using the RAPD technology, Xia et al 28 showed that the Chinese univoltine tetramolter was the earliest tamed wild silkworm, and that the Chinese univoltine trimolter and univoltine tetramolter belonged to two groups with a significant difference in evolution. Lu et al 29 researched silkworm evolution using AFLP, the results confirming that domestic silkworms were evolved from Chinese wild silkworms, and pointed that there were already univoltine, bivoltine and multivoltine silkworms in the early days of domestication, and those silkworms grew into different varieties; Hou et al 22 analysed the evolution of the European univoltine, Japanese univoltine and Chinese bivoltine silkworms with the SSR technique, verifying the theory of mixed origin put forward by Lu et al 29 . Of the 96 objects of this study, there are 63 Chinese univoltine tetramolter varieties, 20 Chinese univoltine trimolter varieties, 11 Chinese bivoltine tetramolter varieties and 2 Japanese bivoltine tetramolter varieties.…”

“…It is generally recognized that the number of repeating units is positively correlated with the number of alleles at the locus. But in silkworm genomes, Reddy et al 15 and Hou et al 22 , discovered that the length of repeated SSR fragments has nothing to do with polymorphism. In this study, the FL0910 and FL1163 amplified fragments of 4 alleles were 234-246 bp and 334-342 bp, respectively.…”

“…As can be seen from dozens of species of animals and plants concerned, the SSR technique can quicken material classification and identification accurately. Our team applied SSR to SSR fingerprint construction for different geographic silkworm varieties and to genetic relationship studies 17,22,23 concluding that SSR could accurately classify silkworm varieties systematically and even analyse the difference in one variety caused by egg colour 23 . Thus it can be seen that the SSR marker technique is an effective method for researching the genetic differences between silkworm varieties.…”

“…As 2nd-generation molecular markers were developed in recent years, Simple Sequence Repeats (SSR) markers have been widely used for molecular map construction, genetic diversity analysis, genetic relationship identification and molecular markers-assisted breeding due to its high stability, polymorphism and efficiency and compliance with the Mendelian genetic law [7][8][9][10][11][12][13] . Reddy et al 14,15 initiated the research of silkworm varieties with SSR markers; Miao et al 16 built an SSR markersbased genetic linkage map for silkworm varieties and mapped a series of silkworm genes based on different phenotype traits [17][18][19][20][21] ; researchers built fingerprints for Chinese, Japanese and introduced silkworm varieties by using SSR markers 22,23 , laying a foundation for the identification and evaluation of silkworm variety resources. However, there remains a lack of related studies on domestic silkworm variety resources with local characteristics.…”