Analysis of Sporopollenin Isolated from Pollen of Ambrosia Artemisiifolia and A. Trifida

Abstract

Sporopollenin was isolated from pollen of Lilium /ongiflorum , Ambrosia trifida and A . artemisiifolia. The sporopollenin was characterized with re spect to its infrared spectrum , elemental composition and nature of potassium hydrox ide fu sion products. Sporopollenin from the Ambrosia species seemed identical. There were only slight differences between Lilium and Ambrosia sporopollenin.

OCCURRENCE OF A RESISTANT BIOPOLYMER IN THE OUTER WALLS OF THE PARASITIC ALGAPROTOTHECA WICKERHAMII(CHLOROCOCCALSES): ULTRASTRUCTURAL AND CHEMICAL STUDIES1

“…As stressed above, no unsaturations were clearly detected in PRP, but the two sporopollenins show a large contribution of carbon-carbon double bonds (absorptions in the range 1650-1600 cm-l) probably trisubstituted (bands at 840-820 cm-I). This large difference in unsaturation levels is also consistent with the ability of PRP to survive prolonged treatments by ethanolamine, a basic reagent promoting oxidative degradation, while numerous sporopollenins are strongly altered by ethanolamine (26,33,36). However, some common features are also noted between PRP and the two sporopollenins, especially the lack of 720 cm-' absorptions and the high level of CH, relative to CH, (although CH, predominance is even more marked in the sporopollenins: 1455/1375 intensity ratio of ca.…”

Receptor‐Independent Sporopollenin

“…Both the smooth surface material and underlying rods may be considered to be composed of sporopollenin but clearly they differ. The results of work on the chemistry and structure of sporopollenin have emphasized the similarity of the polymer from Cambrium times (Zetzsche and Vicari, 1928;Zetzsche, Kalt, Liecht, and Ziegler, 1937;Shaw, 1968, 1977;May, Southworth, and Dickinson, 1975). Basing their conclusions upon earlier results with 2aminoethanol, however, May et al (1975) noted that sporopollenin is heterogeneous since the outer exine dissolves in this organic base while the inner exine zone does not.…”

“…The results of work on the chemistry and structure of sporopollenin have emphasized the similarity of the polymer from Cambrium times (Zetzsche and Vicari, 1928;Zetzsche, Kalt, Liecht, and Ziegler, 1937;Shaw, 1968, 1977;May, Southworth, and Dickinson, 1975). Basing their conclusions upon earlier results with 2aminoethanol, however, May et al (1975) noted that sporopollenin is heterogeneous since the outer exine dissolves in this organic base while the inner exine zone does not. Wiermann and his collaborators have also reported results showing that sporopollenin consists of heterogeneous polymers (Prahl and Wiermann, 1986;Osthoff and Wiermann, 1987).…”