Analysis of Spleen-Induced Fimbria Production in Recombinant Attenuated
            <i>Salmonella enterica</i>
            Serovar Typhimurium Vaccine Strains

Łaniewski, Paweł; Baek, Chang Ho; Roland, Kenneth L.; Curtiss, Roy

doi:10.1128/mbio.01189-17

Cited by 15 publications

(16 citation statements)

References 48 publications

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“…As for Lpf fimbriae, little is known about Sti fimbriae. A study by P. Laniewski et al (46) revealed the involvement of Sti, Saf, Stc and curli fimbriae in murine infection model. Deletion of all four fimbrial operons resulted in a strain attenuated in a murine infection model, but if only one operon was deleted, no effect was observed.…”

Section: Discussionmentioning

confidence: 99%

Factors required for adhesion ofSalmonella entericaserovar Typhimurium to lettuce (Lactuca sativa)

Hensel

Elpers

2020

Preprint

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Salmonella enterica serovar Typhimurium (STM) is a major cause of food-borne gastroenteritis. Recent outbreaks of infections by STM are often associated with non-animal related food, i.e. vegetables, fruits, herbs, sprouts and nuts. One main problem related to consumption of fresh produce is the minimal processing, especially for leafy salads such as corn salad, rocket salad, or lettuce. In this study, we focused on lettuce (Lactuca sativa) which is contaminated by STM at higher rates compared to corn salad, resulting in prolonged persistence. We previously described the contribution of Saf fimbriae, type 1 secretion system (T1SS)-secreted BapA, intact LPS, and flagella-mediated motility to adhesion to corn salad leaves. We systematically analyzed factors contributing to adhesion of STM to lettuce leaves. We used the previously established reductionist, synthetic approach to identify factors that contribute to the surface binding of STM to leaves of lettuce by expressing all known adhesive structure by the Tet-on system. The analyses revealed contributions of Lpf fimbriae, Sti fimbriae, autotransported adhesin MisL, T1SS-secreted BapA, intact LPS, and flagella-mediated motility to adhesion of STM to lettuce leaves. In addition, we identified BapA is a potential adhesin involved in binding to corn salad and lettuce leaf surfaces.ImportanceGastrointestinal pathogens can be transmitted by animal products, as well as by fresh produce of non-animal origin. The numbers of outbreaks by fresh produce contaminated with gastrointestinal pathogens are increasing, and underline the relevance to human health. The mechanisms involved in the colonization of, persistence on, and transmission by fresh produce are poorly understood and have to be part of further research. Here, we investigated the contribution of adhesive factors of STM in the initial phase of plant colonization, i.e. the binding to the plant surface. Usage of a reductionist, synthetic approach including the controlled surface expression of specific adhesive structures of STM, one at a time, allowed the determination of relevant fimbrial and non-fimbrial adhesins, the O-antigen of lipopolysaccharide, the flagella, and chemotaxis to binding to lettuce leaves.

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Section: Discussionmentioning

confidence: 99%

Factors required for adhesion ofSalmonella entericaserovar Typhimurium to lettuce (Lactuca sativa)

Hensel

Elpers

2020

Preprint

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“…Until now, Saf fimbriae were reported to be involved in biofilm formation and in binding to porcine intestine cells IPEC-J2 cells (49). In addition, expression of Saf fimbriae were only observed during infection of murine spleen (50). Genes of the saf operon are often pseudogenes in host-restricted Salmonella serovars (Typhi, Paratyphi and Gallinarum) (10), indicating their potential contribution in STM in dispersal by farm animals and newly investigated environmental routes, e.g.…”

Section: Discussionmentioning

confidence: 99%

Systematic analyses of factors required for adhesion ofSalmonella entericaserovar Typhimurium to corn salad (Valerianella locusta)

Elpers

Kretzschmar

Nuccio

et al. 2019

Preprint

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27Salmonella enterica is a foodborne pathogen leading to gastroenteritis and is commonly 28 acquired by consumption of contaminated food of animal origin. However, numbers of 29 outbreaks linked to the consumption of fresh or minimally processed food of non-animal origin 30 are increasing. New infection routes of S. enterica by vegetables, fruits, nuts and herbs have to 31 be considered. This leads to special interest in S. enterica interactions with leafy products, e.g. 32 salads, that are consumed unprocessed. The attachment of S. enterica to salad is a crucial step 33 in contamination, but little is known about the bacterial factors required and mechanisms of 34 adhesion. S. enterica possesses a complex set of adhesive structures whose functions are only 35 partly understood. Potentially, S. enterica may deploy multiple adhesive strategies for adhering 36 to various salad species, and other vegetables. Here, we systematically analyzed the 37 contribution of the complete adhesiome, of LPS, and of flagella-mediated motility of S. enterica 38 serovar Typhimurium (STM) in adhesion to corn salad. We deployed a reductionist, synthetic 39 approach to identify factors involved in the surface binding of STM to leaves of corn salad with 40 particular regard to the expression of all known adhesive structures using the Tet-on system. 41 This work reveals the contribution of Saf fimbriae, type 1 secretion system-secreted BapA, an 42 intact LPS, and flagella-mediated motility of STM in adhesion to corn salad leaves. 43 44 Importance 45 Human gastrointestinal pathogens are often transmitted by animal products, but recent 46 outbreaks show increasing importance of vegetables as source of infection by pathogenic E. 47 coli or Salmonella enterica. The mechanisms of binding of S. enterica to vegetables such as 48 salad are only poorly understood. We established an experimental model system to 49 systematically investigate the role of adhesive structures of S. enterica serovar Typhimurium 50 in binding to corn salad leaves. The contributions of all members of the complex adhesiome, 51 24.11.2019S. Typhimurium adhesion to corn salad 3 flagella, and O-antigen were evaluated. We identified that Saf fimbriae, type 1 secretion system-52 secreted BapA, an intact LPS, and flagella-mediated motility contribute to adhesion of 53 Salmonella to corn salad leaves. These results will enable future investigations on factors 54 contributing to contamination of vegetables under agricultural conditions. 55 56 24.11.2019 S. Typhimurium adhesion to corn salad Furthermore, SR11 ∆12 strains with additional deletions of single adhesive structures showed 131 no altered levels of adhesion compared to SR11 ∆12, except for deletion of Salmonella 132 157

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“…Heidelberg [29] while it has been found important for pathogenesis in other serotypes such as Salmonella ser. Typhimurium [32]. Transcription of saf fimbrial genes has been found in blood samples from patients infected with Salmonella ser.…”

Section: Antimicrobial Resistance Gene Profiling Of Salmonella Ser Hmentioning

confidence: 99%

Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg

et al. 2018

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Salmonella enterica serotype Heidelberg is primarily a poultry adapted serotype of Salmonella that can also colonize other hosts and cause human disease. In this study, we compared the genomes of outbreak associated non-outbreak causing Salmonella ser. Heidelberg strains from diverse hosts and geographical regions. Human outbreak associated strains in this study were from a 2015 multistate outbreak of Salmonella ser. Heidelberg involving 15 states in the United States which originated from bull calves. Our clinicopathologic examination revealed that cases involving Salmonella ser. Heidelberg strains were predominantly young, less than weeks-old, dairy calves. Pre-existing or concurrent disease was found in the majority of the calves. Detection of Salmonella ser. Heidelberg correlated with markedly increased death losses clinically comparable to those seen in herds infected with S. Dublin, a known serious pathogen of cattle. Whole genome based single nucleotide polymorphism based analysis revealed that these calf isolates formed a distinct cluster along with outbreak associated human isolates. The defining feature of the outbreak associated strains, when compared to older isolates of S. Heidelberg, is that all isolates in this cluster contained Saf fimbrial genes which are generally absent in S. Heidelberg. The acquisition of several single nucleotide polymorphisms and the gain of Saf fimbrial genes may have contributed to the increased disease severity of these Salmonella ser. Heidelberg strains.Electronic supplementary materialThe online version of this article (10.1186/s13099-018-0279-0) contains supplementary material, which is available to authorized users.

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Analysis of Spleen-Induced Fimbria Production in Recombinant Attenuated Salmonella enterica Serovar Typhimurium Vaccine Strains

Cited by 15 publications

References 48 publications

Factors required for adhesion ofSalmonella entericaserovar Typhimurium to lettuce (Lactuca sativa)

Factors required for adhesion ofSalmonella entericaserovar Typhimurium to lettuce (Lactuca sativa)

Systematic analyses of factors required for adhesion ofSalmonella entericaserovar Typhimurium to corn salad (Valerianella locusta)

Genome divergence and increased virulence of outbreak associated Salmonella enterica subspecies enterica serovar Heidelberg

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