Analysis of spermatozoa movement using a video imaging technique

Abstract

The aim of this paper is to present methods for a fully computerised analysis of spermatocyte movement. The technique were designed for processing pure images of spermatozoa captured from a light microscope. The techniques described allowed for the reduction of background light inhomogeneity and for the correct detection of moving cells and involved densitometric equalization of background inhomogeneity and implementation of the dynamic threshold, adopted for the recognition of objects. The method for relating cells (found in each frame processed) to movement trajectories used heuristic rules, describing the behaviour of moving cells. This permitted samples containing high numbers of spermatocytes within the observed area to be processed with maximum accuracy.

Computer-based tracking of single sperm

“…There are also several noncommercial laboratory CASA systems. [4][5][6][7] The percentage of motile sperm and velocity parameters such as swimming speed ͑curvilinear velocity͒, average path velocity, and straight line swimming speed are determined by these systems.…”

EFFECT OF SPERM SUBPOPULATION'S KINETICS ON HUMAN FERTILIZATIONIN VITRO

“…The analyzed area was 640 mm  480 mm size. The following motility parameters were measured: straight line velocity (VSL), cross beat frequency (CBF), lateral head displacement (LHD) and head displacement Sperm Kinetics=IVF 187 along direction of movement observed at VSL velocity or in other name homogeneity of progressive movement velocity (HPMV) [10].…”

“…Sperm motility parameters were measured with the use of ''C-ruch 1.3'' sperm analyzer [10]. Briefly, the analysis was based on the sequences of 51 images registered from the microscope specimen.…”