Analysis of specific misreading in Escherichia coli

Strigini, Paolo; Brickman, Edith

doi:10.1016/0022-2836(73)90299-4

Cited by 72 publications

(39 citation statements)

References 19 publications

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“…The restricting effect is also observed with the strain carrying the strA marker. A similar restriction has already been reported for several other bacterial strains resistant to streptomycin and neamine (Couturier et al, 1964;Gorini, 1967;DeWilde et al, 1975;Bollen et al, 1975 Biochemical analysis of the mutant strain RH3128 It has been observed by other workers that misreading in protein-synthesizing systems in vitro (Davies et al, 1964;DeWilde et al, 1975;Bollen et al, 1975) is decreased in some mutants resistant to streptomycin and neamine, and this low misreading has been correlated with restriction in vivo (Strigini & Brickman, 1973 The 50S ribosomal subunits used for re-association of 70S ribosomes were of parental origin in all experiments, and other components were as follows: O, 16S rRNA from strain RH3113 and TP30 from strain RH3113; A, 16S rRNA from strain RH3113 and TP30 from strain RH3128; *, 16S rRNA from strain RH3128 and TP30 from strain RH3128;…”

Section: Bacterial Strainssupporting

confidence: 81%

Resistance to the aminoglycoside antibiotic neamine in Escherichia coli. A new mutant whose NeaR phenotype results from the cumulative effects of two distinct mutations

Delcuve¹,

Cabezón²,

Herzog³

et al. 1978

Biochemical Journal

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A spontaneous mutant of Escherichia coli (strain AB2847), selected for resistance to the aminoglycoside antibiotic neamine, shows severe restriction of amber suppressors in vivo. Ribosomes isolated from the mutant exhibit only low misreading in vitro in the presence of the antibiotic. Genetic and biochemical analyses indicate that the neamine-resistant phenotype is the result of two distinct mutations. The first, res3128, appears to affect the gene (strA) coding for the ribosomal protein S12. Although it leads to a restrictive phenotype it does not, however, confer resistance to streptomycin. The second mutation, X3128, is located between the strA and aroB loci and is lethal when segregated from the res3128 mutation. It may affect the ribosome at the level of a post-translational modification.

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Section: Bacterial Strainssupporting

confidence: 81%

Resistance to the aminoglycoside antibiotic neamine in Escherichia coli. A new mutant whose NeaR phenotype results from the cumulative effects of two distinct mutations

Delcuve¹,

Cabezón²,

Herzog³

et al. 1978

Biochemical Journal

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“…This was further restricted with certain base neighbours [6,45]. This selectivity may not necessarily apply to the situation in vivo, although a necessarily limited study of the strepto-mycin-induced misreading in vivo of all three nonsense codons by a nonsense tRNA suppressor indicated it to be the same [46]. c) Due to the degeneracy of the code [47], not all misreadings result in missense.…”

Section: Discussionmentioning

confidence: 99%

“…Logarithmically growing wildtype cells have a low background level of ambiguity in translation. This was thought to be of the order of 0.1 % by Gorini [48] or rather more, namely 1.8 % based on the extent of translational leakiness causing suppression of a nonsense mutation [46]. Strains carrying tRNA suppressors or the ram mutation [49] have additional mistranslation effects.…”

Section: Discussionmentioning

confidence: 99%

An Investigation of Mistranslation in vivo Induced by Streptomycin by an Examination of the Susceptibility of Abnormal Proteins to Degradation

Hewitt

Kogut

1977

European Journal of Biochemistry

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Proteolysis rates in vivo were measured in Escherichia coli cultures during treatment with dihydrostreptomycin and under various other conditions. Dihydrostreptomycin treatment caused an increase in the proteolysis rate, compared to untreated controls. The proteolytic system in vivo responsible for the elevated proteolysis in the early stages of dihydrostreptomycin treatment, or that during canavanine and puromycin treatment, were not inhibited by addition of phenylniethanesulphonyl fluoride. This agent did inhibit proteolysis rates in cultures whose growth was inhibited by starvation, or had been completely stopped by dihydrostreptomycin. It seems, therefore, that the extremely high proteolysis rates in cultures at this stage of dihydrostreptomycin treatment were due to the action of two protease systems: the one concerned with the breakdown of abnormal proteins, and the other concerned with normal protein turnover and active during a non-specific decline of growth.The proteolytic rate at complete growth inhibition brought about by dihydrostreptomycin was intermediate between those induced by canavanine and puromycin at the same stage of treatment. This indicated a similar hierarchy in the extent and nature of abnormality in the proteins synthesised under these conditions. The relationship between the abnormality of proteins induced by dihydrostreptomycin and the importance of this in the antibiotic mechanism is discussed.Streptomycin was demonstrated to cause mistranslation during protein synthesis in vitro with synthetic [l] and natural messengers [2-41. Since in vitro, the misreading effect of streptomycin is effective at the level of codon-anticodon interaction [ 5 ] and is limited to certain bases [6], one can envisage that streptomycin-induced misreading could occur to cause both missense and nonsense. The misreading effect was used to account for the phenotypic suppression in vivo of both nonsense and missense mutations in streptomycin-resistant and streptomycinsensitive Escherichia coli strains treated with streptomycin 17-12]. Misreading has been proposed as effective in the growth inhibitory effect of streptomycin, but this hypothesis has been contested [S]. On the other hand, indications of misreading effects of streptomycin being related to the growth inhibitory effects are supported by the demonstration of growth of mutants by phenotypic suppression at low streptomycin concentrations, whereas at higher streptomycin concentrations there is non-growth of such mutants [ l l -121. Further, a correlation has been found between the extent of misreading in vitro caused by streptomycin, paramomycin or ethanol [ 1,2,13] and the response of strains sensitive, resistant or dependent on streptomycin to these agents in vivo [ 141. This is particularly pertinent for streptomycindependent strains which are resistant to growth inhibition in the presence of streptomycin or paramomycin, but sensitive to their combined presence. Also, in a protein-synthesising system in vitro, the polypeptides produced were smaller ...

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“…Nevertheless, there is evidence that such suppression takes place although the efficiency is only a few percent of that of the amber suppression (6,7). This apparent contradiction can be resolved by assuming that the amber suppressor can read the ochre codon, by using the "two out of three" method, with a probability that is sufficient to sustain the low suppression observed.…”

mentioning

confidence: 99%

"Two out of three": an alternative method for codon reading.

Lagerkvist¹

1978

Proc. Natl. Acad. Sci. U.S.A.

248

109

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An alternative method for codon reading, whereby only the first two codon nucleotides are recognized by the anticodon, is discussed and the experimental evidence for this "two out of three" reading method is reviewed. Misreading of codons by the "two out of three" method could pose a significant threat to ihe fidelity of rotein synthesis unless the genetic code is organized in suc-a way as to prevent this method from being used when it might compromise translational fidelity. Inspection of the genetic code shows that it is arranged in such a way that the "two out of three" reading method can be used without translational errors.

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Analysis of specific misreading in Escherichia coli

Cited by 72 publications

References 19 publications

Resistance to the aminoglycoside antibiotic neamine in Escherichia coli. A new mutant whose NeaR phenotype results from the cumulative effects of two distinct mutations

Resistance to the aminoglycoside antibiotic neamine in Escherichia coli. A new mutant whose NeaR phenotype results from the cumulative effects of two distinct mutations

An Investigation of Mistranslation in vivo Induced by Streptomycin by an Examination of the Susceptibility of Abnormal Proteins to Degradation

"Two out of three": an alternative method for codon reading.

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