Analysis of specific antibody and cellular immune response to first-dose measles vaccine Edmonston-Zagreb (EZ) in 9-month-old infants

Hr, Teni Nurlatifah; Barlıanto, Wisnu; Wiyasa, I Wayan Arsana; Kusuma, H.M.S. Chandra; Sari, Tita Luthfia; Bachtıar, Novilia Sjafri

doi:10.15586/aei.v49i3.6

Cited by 3 publications

(3 citation statements)

References 23 publications

(33 reference statements)

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“…Regarding allergenicity, domain III is the most important since it contains both IgE- and IgG-binding epitopes, making it a primary determinant of egg allergy [ 229 , 230 ]. OVM retains its allergenic reactivity even after being subjected to methods that permanently disrupt protein structure, such as heat treatment (e.g., 100 °C for 30 min) or chemical treatment with denaturing detergents (e.g., urea) [ 233 , 234 ]. When compared to OVM, OVA is a low allergen.…”

Section: Allergenicity From Egg Proteinsmentioning

confidence: 99%

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

et al. 2023

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This review article discusses advanced extraction methods to enhance the functionality of egg-derived peptides while reducing their allergenicity. While eggs are considered a nutrient-dense food, some proteins can cause allergic reactions in susceptible individuals. Therefore, various methods have been developed to reduce the allergenicity of egg-derived proteins, such as enzymatic hydrolysis, heat treatment, and glycosylation. In addition to reducing allergenicity, advanced extraction methods can enhance the functionality of egg-derived peptides. Techniques such as membrane separation, chromatography, and electrodialysis can isolate and purify specific egg-derived peptides with desired functional properties, improving their bioactivity. Further, enzymatic hydrolysis can also break down polypeptide sequences and produce bioactive peptides with various health benefits. While liquid chromatography is the most commonly used method to obtain individual proteins for developing novel food products, several challenges are associated with optimizing extraction conditions to maximize functionality and allergenicity reduction. The article also highlights the challenges and future perspectives, including optimizing extraction conditions to maximize functionality and allergenicity reduction. The review concludes by highlighting the potential for future research in this area to improve the safety and efficacy of egg-derived peptides more broadly.

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Section: Allergenicity From Egg Proteinsmentioning

confidence: 99%

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

et al. 2023

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“…According to prior research, this antibody assay was assessed utilizing a plaque-reduction microneutralization technique and a recombinant measles virus strain [24]. Seropositivity was defined as an antibody level of 120 mIU/ml (disease protection was assumed) [25].…”

Section: Measles-specific Neutralizing Antibody Assaymentioning

confidence: 99%

“…The determination of measles-specific T cells producing IFN-γ was performed according to a previous studies [24], [26]. Surface antibodies against CD8 (PE/ Cy5 Anti-human CD8a Antibody, Biolegend, cat.no.…”

Section: Flow Cytometrymentioning

confidence: 99%

The Role of Sex, Ethnicity, Age, and Nutritional Status in the Seropositivity of the Measles Vaccine

Nurlatifah

Barlıanto

Wiyasa

et al. 2021

Open Access Maced J Med Sci

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AIM: This study investigates the relationship between sex, ethnicity, age, nutritional status with the seropositivity of the Edmonston-Zagreb vaccine in children. METHODS: A cross sectional, observational study was conducted. A total of 45 children were differentiated based on sex, ethnicity, age, and nutritional status when they received the Edmonston-Zagreb measle vaccine for the first time. Flow cytometry was used to look at differences in antibody status as well as populations of CD-4 and CD-8 cells that release IFN- γ. RESULTS: We found no significant differences in antibody levels or CD-4 and CD-8 cell populations that secrete IFN- γ between boys and girls (p > 0.05). Besides, similar results were also confirmed in comparisons between Javanese and Sundanese ethnic groups, 9 months versus more than 9 months of age, or normal versus low body mass index (p > 0.05). CONCLUSIONS: We conclude that sex, race, age, and nutritional status had no effect on immune response to vaccination. As a result, there was no barrier to seroconversion and optimal immunological performance in the children in this trial who received the Edmonston-Zagreb measles vaccination.

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Determination Factors Associated with Immune Responses Towards First Dose Edmonston-Zagreb (EZ) Measles Vaccine in Indonesian Infants

Nurlatifah¹,

Barlıanto²,

Wiyasa³

et al. 2021

Med Arch

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Background: Measles outbreaks under high coverage of measles vaccination in Indonesia is gaining a necessary evaluation of factors associated with measles-vaccine immune responses. Objctive: The purposes of recent study were to evaluate the association of sociodemographic and anthropometric parameters and specific immune responses towards first dose Edmonston-Zagreb (EZ) measles vaccine in Indonesian infants. Methods: A total of 35 infants were enrolled in this cohort study. Measles immune responses were followed up at one and six months after vaccination then analyzed for its association with sociodemographic, anthropometric, and nutritional parameters. The plaque-reduction microneutralization assay was conducted to measure the titer of measles specific IgG antibody. The level of CD4 + and CD8 + T-cells that exhibiting gamma interferon (IFN-γ) secretion were analyzed by flow cytometry. The association between variable was analyzed by linear regression. The difference immune response among variable were analyzed with Mann-Whitney test. Results: Vitamin A supplementation and breastfeeding were predicted as associated factor for humoral and cellular immune response after one month and six months measles vaccination among Indonesia Infants. Conclusion: Nutritional factor is associated with measles vaccination immune response in Indonesian infants.

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Analysis of specific antibody and cellular immune response to first-dose measles vaccine Edmonston-Zagreb (EZ) in 9-month-old infants

Cited by 3 publications

References 23 publications

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

The Impact of Processing and Extraction Methods on the Allergenicity of Targeted Protein Quantification as Well as Bioactive Peptides Derived from Egg

The Role of Sex, Ethnicity, Age, and Nutritional Status in the Seropositivity of the Measles Vaccine

Determination Factors Associated with Immune Responses Towards First Dose Edmonston-Zagreb (EZ) Measles Vaccine in Indonesian Infants

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