Analysis of some antifungal drugs by spectrophotometric and spectrofluorimetric methods in different pharmaceutical dosage forms

Khashaba, Pakinaz Y.; El-Shabouri, Salwa R; Emara, Kamla M.; Mohamed, Ashraf M.

doi:10.1016/s0731-7085(99)00280-0

Cited by 86 publications

(46 citation statements)

References 15 publications

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“…However, the analysis of posaconazole required a lengthy chromatography due to the late elution of itraconazole (>14 min). After scrutinizing reports for ketoconazole, it was found to exhibit native fluorescent properties at 345-440 nm with excitation at 240-320 nm [36,37]. As such, ketoconazole was selected and proved to be an excellent alternative which allowed for a shorter chromatography time (<8 min).…”

Section: Internal Standard Alternativementioning

confidence: 99%

Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection

Tang

2017

Separations

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Abstract:A sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of posaconazole in human plasma/serum. The method is based on a single dilution step by treating the sample with methanol, and followed by the direct injection of the sample into the HPLC system. Posaconazole and internal standard ketoconazole in the methanol extract are subsequently analyzed by using a fluorescence (FL) detector at optimized wavelengths (excitation 245 nm and emission 380 nm). The method achieves a linear detector response for peak height measurements over the concentration range of 0.1-10 µg/mL which adequately covers the therapeutic range for appropriate patient monitoring. The chromatographic time is less than 8 min per injection, an improvement over most published HPLC/FL or HPLC/UV methods. The method's limit of quantitation, linearity, imprecision, and accuracy met all criteria required by the Guidance for Industry Bioanalytical Method Validation. In comparison to other published methods, the current method would be of interest to analytical and clinical laboratories because it employs simple, rapid, and cost-effective procedures.

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Section: Internal Standard Alternativementioning

confidence: 99%

Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection

Tang

2017

Separations

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“…The slit width was Various methods are available for the analysis of the two drugs in the literature separately; hydrocortisone; UV-spectrophotometer 10 , HPLC [11][12][13][14] , TLC 13 and LC-MS/MS 15 ; clotrimazole; UV-spectrophotometer 16 , Titration 17 , HPLC & TLC 13 . However, there is no report on analytical methods for the determination of hydrocortisone and clotrimazole simultaneously and that resolve the degradation products from the parent drugs.…”

Section: Instrumentation and Chromatographic Conditionmentioning

confidence: 99%

Development and Validation of HPTLC Assay Method for Simultaneous Quantification of Hydrocortisone and Clotrimazole in Cream and Applying for Stability Indicating Test

Genete

Hymete

Bekhit

2012

J. Chil. Chem. Soc.

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A simple, specific, precise and accurate simultaneous high-performance thin-layer chromatographic method of analysis for hydrocortisone and clotrimazole was developed and validated. The method employed HPTLC glass plates (20x10) mm precoated with silica gel 60 F 254 as the stationary phase. The solvent system consisted of Toluene: Propanol: Ammonia (13:3:0.1v/v) that gave compact spots with R f value of 0.27 ± 0.01 and 0.58 ± 0.02 for hydrocortisone and clotrimazole, respectively. Densitometric analysis of hydrocortisone and clotrimazole was carried out in the absorbance/reflectance mode at 226nm. The calibration curves showed good linear relationship with R 2 = 0.996 + 0.003 and 0.996 + 0.002 in the concentration range of 200-1200 and 200-1000 ng/µl for hydrocortisone and clotrimazole, respectively. The method was validated for precision, recovery and robustness. The LOD & LOQ were found to be 35.31, 107.01 and 34.93, 105.87 ng/µl for hydrocortisone and clotrimazole respectively. Statistical analysis proved that the method is repeatable, specific and accurate for the estimation of the studied drugs. Resolution of hydrocortisone, clotrimazole and their degradation products formed under different stress conditions (acid-base hydrolysis, oxidation and thermal degradation) was successfully achieved with the developed method indicating that it can be employed as a stability indicating method. Hydrocortisone (cortisol);11,17,20-dione, the main physiologic glucocorticoid in humans has an anti-inflammatory and an immunosuppressive effect besides its role on carbohydrate and protein metabolism 1-3 . The anti-inflammatory effect of hydrocortisone is due to reduction of histamine release, reduction of IgG production and decreasing of the activity of neutrophils and macrophages [4][5][6]imidazole is an imidazole derivative with antimycotic activity that is known to inhibit cytochrome P-450, ergosterol biosynthesis, and proliferation of cells and to interfere with cellular Ca 2+ homeostasis 7-9 . BACKGROUND

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“…The charge transfer reactions of certain -acceptors have been utilized successfully in pharmaceutical analysis. Chloranilic acid is a ττ-acceptor used for the analysis of certain penicillines [25,26] , cephalosporines [27,28] , imipramine [29] , antimalarials [30] , antifungal [31] and propranolol [32] . A review of literature revealed that no spectrophotometric method for determination of tetracycline and oxytetracycline through charge transfer complexation with chloranilic acid has been reported.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Certain Tetracyclines and Oxytetracyclines through Charge Transfer Complexation

Fahelelbom¹

2008

American J. of Pharmacology and Toxicology

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Problem statement: Several studies were carried out to develop and validate a simple, rapid and accurate spectrophotometric method for the analysis of tetracycline (TC) and oxytetracycline (OTC) in the pure form and in pharmaceutical dosage forms, despite the varieties of these analytical techniques, the literature revealed that no spectrophotometric method for determination of tetracycline and oxytetracycline using charge transfer complexation with chloranilic acid has been reported. Approach: In the present study, a new and simple spectrophotometric method is described for the determinations of tetracycline (TC) and oxytetracycline (OTC), the method is based on the molecular interaction between the tetracyclines and Chloranilic Acid (CA), to form a charge-transfer complex in which the drugs acts as n-donor and chloranilic acid as -acceptor in acetonitrile. The spectra, various experimental parameters for the reaction, the stability of the complex and the validty of the method were investigated. Results: The formed complex was found to absorb maximally at 540nm, Beer's law is obeyed in the concentration ranges 2.5-30 µg mL −1 and 5.0-40 µg mL −1 for TC and OTC, respectively. The limits of detection (S/N = 3) were 0.40 µg mL −1 for tetracycline and 0.50 µg mL −1 for oxytetracycline. The molar ratio was found to be approximately 1:1, confirming that one molecule of tetracycline or oxytetracycline combines with one molecule of chloranilic acid. Conclusions and Recommendations: The proposed method was found to be rapid, accurate and sensitive and may be applied for estimation of named drugs in pharmaceutical dosage forms without interferences from the common additives encountered. Percentage recoveries ranged from 99.17%±0.95-100.38%±0.82. From the statistical analysis of the obtained results one can conclude that no significant difference between the proposed method and other official methods as evident from the t-test and variance ratio. These findings recommend the utility of the proposed method in the quality control analysis of TC and OTC in the pure form and pharmaceutical dosage forms.

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Analysis of some antifungal drugs by spectrophotometric and spectrofluorimetric methods in different pharmaceutical dosage forms

Cited by 86 publications

References 15 publications

Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection

Determination of Posaconazole in Plasma/Serum by High-Performance Liquid Chromatography with Fluorescence Detection

Development and Validation of HPTLC Assay Method for Simultaneous Quantification of Hydrocortisone and Clotrimazole in Cream and Applying for Stability Indicating Test

Analysis of Certain Tetracyclines and Oxytetracyclines through Charge Transfer Complexation

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