Analysis of Skeletal Muscle Development in Drosophila

Morriss, Ginny R.; Bryantsev, Anton L.; Chechenova, Maria B.; LaBeau, Elisa M.; Lovato, TyAnna L.; Ryan, Kathryn M.; Cripps, Richard M.

doi:10.1007/978-1-61779-343-1_8

Cited by 22 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…#11175025910). Hybridization was carried out as described (Morriss et al, 2012). Images were obtained with a Zeiss LSM510-Meta confocal microscope.…”

Section: Methodsmentioning

confidence: 99%

Wnt4 is required for ostia development in the Drosophila heart

Chen

Zhu

et al. 2016

Developmental Biology

View full text Add to dashboard Cite

The Drosophila ostia are valve-like structures in the heart with functional similarity to vertebrate cardiac valves. The Wnt/ -catenin signaling pathway is critical for valve development in zebrafish and mouse, but the key ligand(s) for valve induction remains unclear. We observed high levels of Wnt4 gene expression in Drosophila ostia progenitor cells, immediately prior to morphological differentiation of these cells associated with ostia formation. This differentiation was blocked in Wnt4 mutants and in flies expressing canonical Wnt signaling pathway inhibitors but not inhibitors of the planar cell polarity pathway. High levels of Wnt4 dependent activation of a canonical Wnt signaling reporter was observed specifically in ostia progenitor cells. In vertebrate valve formation Wnt signaling is active in cells undergoing early endothelial-mesenchymal transition (EMT) and the Wnt9 homologue of Drosophila Wnt4 is expressed in valve progenitors. In demonstrating an essential role for Wnt4 in ostia development we have identified similarities between molecular and cellular events associated with early EMT during vertebrate valve development and the differentiation and partial delamination of ostia progenitor cells in the process of ostia formation.

show abstract

“…#11175025910). Hybridization was carried out as described (Morriss et al, 2012). Images were obtained with a Zeiss LSM510-Meta confocal microscope.…”

Section: Methodsmentioning

confidence: 99%

Wnt4 is required for ostia development in the Drosophila heart

Chen

Zhu

et al. 2016

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…Cryosectioning, Immunofluorescence, and Microscopy-Cryosectioning and immunostaining of adult flies were performed as described before (17). Antibodies to ␣-actinin, Kettin (also known as Titin and Sallimus (Sls)), and TpnC were obtained from Abcam.…”

Section: Methodsmentioning

confidence: 99%

The Drosophila Z-disc Protein Z(210) Is an Adult Muscle Isoform of Zasp52, Which Is Required for Normal Myofibril Organization in Indirect Flight Muscles

Chechenova

Bryantsev

Cripps

2013

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

“…White pre-pupae (0 ± 1h APF) were collected and aged for 44 hours at 25°C (44h APF). Pupal muscle dissections were preformed as described previously (Morriss et al, 2012). Cutting along the dorsal midline allowed the visualization of the abdominally developing ventral muscles.…”

Section: Methodsmentioning

confidence: 99%

Cell death regulates muscle fiber number

Sarkissian

Arya

Gyonjyan

et al. 2016

Developmental Biology

View full text Add to dashboard Cite

Cell death can have both cell autonomous and non-autonomous roles in normal development. Previous studies have shown that the central cell death regulators grim and reaper are required for the developmentally important elimination of stem cells and neurons in the developing central nervous system (CNS). Here we show that cell death in the nervous system is also required for normal muscle development. In the absence of grim and reaper, there is an increase in the number of fibers in the ventral abdominal muscles in the Drosophila adult. This phenotype can be partially recapitulated by inhibition of cell death specifically in the CNS, indicating a non-autonomous role for neuronal death in limiting muscle fiber number. We also show that FGFs produced in the cell death defective nervous system are required for the increase in muscle fiber number. Cell death in the muscle lineage during pupal stages also plays a role in specifying fiber number. Our work suggests that FGFs from the CNS act as a survival signal for muscle FCs. Thus, proper muscle fiber specification requires cell death in both the nervous system and in the developing muscle itself.

show abstract

Analysis of Skeletal Muscle Development in Drosophila

Cited by 22 publications

References 30 publications

Wnt4 is required for ostia development in the Drosophila heart

Wnt4 is required for ostia development in the Drosophila heart

The Drosophila Z-disc Protein Z(210) Is an Adult Muscle Isoform of Zasp52, Which Is Required for Normal Myofibril Organization in Indirect Flight Muscles

Cell death regulates muscle fiber number

Contact Info

Product

Resources

About