Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography

Guerrant, G O; Lambert, M A; Moss, C W

doi:10.1128/jcm.16.2.355-360.1982

Cited by 155 publications

(53 citation statements)

References 13 publications

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“…The organic acid anions are mainly derived from microbial catabolism and hence these agents (either individually or two or more in concert) conceivably serve as chemotaxonomic markers of microbial activity in the oral environment. For example, n-butyrate is generated by the pathogenic micro-organism P. gingivalis [18].…”

Section: High-field High Resolution Proton Nmr Analysis Of Human Salivamentioning

confidence: 99%

High Resolution ¹H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

2006

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High resolution proton (1H) nuclear magnetic resonance (NMR) spectroscopy was employed to simultaneously evaluate the oxidising actions of ozone (O3) towards a wide range of salivary biomolecules in view of its applications in dental practices, which may serve as a viable and convenient means for the treatment of dental caries. Treatment of supernatants derived from unstimulated human saliva specimens (n=12) with O3 (4.48 mmol) revealed that this reactive oxygen species gave rise to the oxidative consumption of pyruvate (generating acetate and CO2 as products), lactate (to pyruvate and sequentially acetate and CO2), carbohydrates in general (a process generating formate), methionine (giving rise to its corresponding sulphoxide), and urate (to allantoin). Further, minor O3-induced modifications included the oxidation of trimethylamine and 3-D-hydroxybutyrate, the fragmentation of salivary glycosaminoglycans to NMR-detectable saccharide fragments, and the conversion of polyunsaturated fatty acids to their ozonides. Moreover, evidence for the ability of O3 to induce the release of selected low-molecular-mass salivary biomolecules from macromolecular binding-sites was also obtained. Since many of the oxidation products detectable in O3-treated samples are identical to those arising from the attack of *OH radical on biofluid components, it appears that at least some of the modifications observed here are attributable to the latter oxidant (derived from O3*- generated from the single electron reduction of O3).

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Section: High-field High Resolution Proton Nmr Analysis Of Human Salivamentioning

confidence: 99%

High Resolution ¹H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

2006

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“…High performance liquid chromatography (HPLC) was used for acidic end-product analysis following the method of Guerrant et al [12]. Ethanol was assayed using an Ethanol kit (Boehringer, Mannheim, Germany).…”

Section: Analysis Of Fermentation End-productsmentioning

confidence: 99%

The response to oxidative stress of Fusobacterium nucleatum grown in continuous culture

Diaz¹

2000

FEMS Microbiology Letters

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Fusobacterium nucleatum ATCC 10953 was grown in continuous culture and the atmosphere changed stepwise from nitrogen (anaerobiosis) to a mixture of air: oxygen (40:60). No significant differences in biomass were observed and the baseline low level of superoxide dismutase increased only slightly; catalase and peroxidase activities were never detected but the level of NADH oxidase increased more than three-fold when oxygen was introduced into the system. In relation to acidic end-products, the relative proportion of acetate increased while that of butyrate decreased. Due mainly, it would seem, to NADH oxidase activity, the culture maintained a low redox potential (E h = 3274 mV) even under an atmosphere of 40% oxygen in air and dissolved oxygen was not detected. This may, in part, explain the protective role of F. nucleatum for anaerobes in both biofilm and planktonic phases of aerated, mixed cultures of oral bacteria. ß

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“…Determination of proline was achieved using the modified derivatization method of Cooper & Turnell (4), and cystine by the method of Cooper et al (5). Glucose and organic acids were assayed (19) by ion exclusion high-performance liquid chromatography using refractive index detection based on the method of Guerrant et al (10). Nitrite was determined using the method of Canney et al (2).…”

Section: Analytical Techniquesmentioning

confidence: 99%

Some observations on the nutritional requirements of Eikenella corrodens ATCC 23834^T grown in continuous culture

Gully

Rogers

1995

Oral Microbiology and Immunology

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The type strain, ATCC 23834, of Eikenella corrodens was grown anaerobically in continuous culture in chemically defined media. Initial experiments showed that glucose was not utilized and it was subsequently omitted from all media. The initial chemically defined medium contained varying levels of 14 amino acids and 20 mM potassium nitrate, an essential nutrient for growth under these conditions. In this medium, the doubling time was 2.1 h, the optimum growth temperature 34 degrees C and the pH 7.2. The key growth-promoting amino acids were glutamate and serine, and the culture appeared to be nitrate limited. By growing the organism in a series of chemically defined media containing 40 mM nitrate and varying levels of these amino acids, glutamate was found to be the major contributor to biomass increase. Its catabolism may be linked to a respiratory chain involving nitrate as the ultimate electron acceptor.

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Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography

Cited by 155 publications

References 13 publications

High Resolution ¹H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

High Resolution ¹H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

The response to oxidative stress of Fusobacterium nucleatum grown in continuous culture

Some observations on the nutritional requirements of Eikenella corrodens ATCC 23834^T grown in continuous culture

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Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography

Cited by 155 publications

References 13 publications

High Resolution 1H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

High Resolution 1H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

The response to oxidative stress of Fusobacterium nucleatum grown in continuous culture

Some observations on the nutritional requirements of Eikenella corrodens ATCC 23834T grown in continuous culture

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High Resolution ¹H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

High Resolution ¹H NMR investigations of the oxidative consumption of salivary biomolecules by ozone: Relevance to the therapeutic applications of this agent in clinical dentistry

Some observations on the nutritional requirements of Eikenella corrodens ATCC 23834^T grown in continuous culture