Analysis of Serum microRNA Profile by Solexa Sequencing in Women With Endometriosis

Wang, Lei; Huang, Wei; Ren, Chao; Zhao, Ming; Jiang, Xingjun; Fang, Xiaoling; Xia, Xiaomeng

doi:10.1177/1933719116641761

Cited by 61 publications

(65 citation statements)

References 23 publications

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“…Many microRNAs have been investigated in the study of endometriosis. Wang et al . evaluated microRNA expression in serum samples from 30 women with minimal to mild endometriosis and 20 control women.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis

Maged

Deeb

Amir

et al. 2017

Intl J Gynecology & Obste

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Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis

Maged

Deeb

Amir

et al. 2017

Intl J Gynecology & Obste

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“…Studies have found that the miRNAs obtainable from diseased tissues and other body fluids were able to detect various diseases. [ 40 ] Today, advances in sequencing and microarray technology have made it possible to investigate systemic levels of miRNAs [ 41 ] and long non-coding RNA. [ 42 ] Deregulation of miRNAs is involved in the pathophysiology of endometriosis and they have been investigated as potential biomarkers.…”

Section: New Molecular Signaturesmentioning

confidence: 99%

Current biomarkers for the detection of endometriosis

Tian

Chang

Zhao

et al. 2020

Chinese Medical Journal

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A clinically reliable non-invasive test for endometriosis is expected to reduce the diagnostic delay. Although varieties of biomarkers have been investigated for decades, and cancer antigen-125, cancer antigen-199, interleukin-6, and urocortin were the most studied ones among hundreds of biomarkers, no clinically reliable biomarkers have been confirmed so far. Some emerging technologies including “omics” technologies, molecular imaging techniques, and microRNAs are promising in solving these challenges, but their utility to detect endometriosis has yet to be verified. New combinations of researched indicators or other non-invasive methods and further exploration of the emerging technologies may be new targets and future research hotspots for non-invasive diagnosis of endometriosis. In conclusion, researches of biomarkers for the detection of endometriosis are still ongoing and may benefit from novel molecular biology, bioinformatics methods and a combination of more diverse monitoring methods. Though it will be a daunting task, the identification of a specific set of diagnostic biomarkers will undoubtedly improve the status of endometriosis.

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“…Studies investigating miRNA profiles in eutopic tissues from women with and without endometriosis [64], [65], [66] showed a trend of downregulated miRNA levels in tissues from women with endometriosis. Wang and colleagues also showed global downregulation in the circulating levels of miRNAs in the serum of women with endometriosis, with 91% of significantly differentiated miRNAs showing decreased expression in endometriosis patients [67]. There are very few studies that have measured miRNAs in the peritoneal fluid (PF), which is the most dynamic component and major player in the etiology of endometriosis [11], [68], [69].…”

Section: Introductionmentioning

confidence: 99%

Redox regulation of microRNAs in endometriosis-associated pain

2017

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Endometriosis is a chronic, painful condition with unknown etiology. A differential expression of microRNAs in the endometriotic tissues from women with endometriosis with pain compared to those without suggested a plausible role for miRNA or epigenetic mechanisms in the etiology of endometriotic pain. The peritoneal milieu is involved in maintenance of endometriotic lesion and nociception. We recently showed the mechanistic role for oxidized-lipoproteins (ox-LDLs) present in peritoneal fluid (PF) in endometriosis and pain. We explored the possibility of ox-LDLs modulating the expression of miRNAs in a manner similar to PF from women with endometriosis. Expression levels of miRNAs and their predicted nociceptive and inflammatory targets were determined in PF and ox-LDL treated human endometrial cell-lines. Samples from IRB-approved and consented patients with and without endometriosis or pain were used. These were compared to endometrial cell-lines treated with various forms of oxidized-lipoproteins. RNA (including miRNAs) were isolated from treated endometrial cells and expression levels were determined using commercial miRNome arrays. Cell lysates were used in immunoblotting for inflammatory proteins using a protein array. Twenty miRNAs including isoforms of miR-29, miR-181 and let-7 were mutually differentially expressed in cells treated with PF from endometriosis patients with pain and those treated with ox-LDL components. The ox-LDLs and endo-PF treatment also produced significant overexpression of microRNA predicted target genes nerve growth factor, interleukin-6 and prostaglandin E synthase and overexpression of their downstream protein targets Mip1α and MCP1. This study showed similarities between miRNA regulation in PF from endometriotic women and ox-LDLs present in abundance in the PF of these women. Key miRNAs responsible for targeting nociceptive and inflammatory molecules were downregulated in the presence of ox-LDLs and endo-PF, thus playing a role in the etiology of endometriotic pain. These redox-sensitive miRNAs can be of potential use as targets in the treatment of endometriosis-associated pain.

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Analysis of Serum microRNA Profile by Solexa Sequencing in Women With Endometriosis

Abstract: Circulating miRNAs may be useful as detection biomarkers for the early diagnosis of minimal-mild endometriosis.

Cited by 61 publications

References 23 publications

Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis

Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis

Current biomarkers for the detection of endometriosis

Redox regulation of microRNAs in endometriosis-associated pain

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