Analysis of serotypes and electropherotypes of equine rotaviruses isolated in the United States

Hardy, Michele E.; Woode, G. N.; Xu, Zhichang; Williams, John D.; Conner, Margaret E.; Dwyer, Roberta M.; Powell, D G

doi:10.1128/jcm.29.5.889-893.1991

Cited by 27 publications

(20 citation statements)

References 16 publications

(17 reference statements)

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“…Six G types and 6 P types have been described among equine rotaviruses to date (Table 2). However, the majority of circulating equine rotaviruses are G3P[12] and G14P[12] (Browning et al, 1992a;Browning and Begg, 1996;Ciarlet et al, 1994;Collins et al, 2008;Elschner et al, 2005;Garaicoechea et al, 2011;Hardy et al, 1991;Isa et al, 1996;Monini et al, 2011;Nemoto et al, 2011;Ntafis et al, 2010;Ohta et al, 1990;Tsunemitsu et al, 2001;van der Heide et al, 2005).…”

Section: Epidemiology and Aetiologymentioning

confidence: 99%

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Equine rotaviruses—Current understanding and continuing challenges

Bailey

Gilkerson

Browning

2013

Veterinary Microbiology

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Equine rotaviruses were first detected in foals over 30 years ago and remain a major cause of infectious diarrhoea in foals. During this time, there has been substantial progress in the development of sensitive methods to detect rotaviruses in foals, enabling surveillance of the genotypes present in various horse populations. However, there has been limited epidemiological investigation into the significance of these circulating genotypes, their correlation with disease and the use of vaccination in these animal populations. Our knowledge of the pathogenesis of rotavirus infection in foals is based on a limited number of studies on a small number of foals and, therefore, most of our understanding in this area has been extrapolated from studies in other species. Questions such as the concentrations of rotavirus particles shed in the faeces of infected foals, both with and without diarrhoea, and factors determining the presence or absence of clinical disease remain to be investigated, as does the relative and absolute efficacy of currently available vaccines. The answer to these questions may help direct research into the development of more effective control measures.

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Section: Epidemiology and Aetiologymentioning

confidence: 99%

“…In a typical equine group A rotavirus pattern (Fig. 2), gene segments 3 and 4 migrate close together, and segments 7, 8, and 9 form a well spaced triplet (Hardy et al, 1991;Ohta et al, 1990;.…”

Section: Diagnosismentioning

confidence: 99%

Equine rotaviruses—Current understanding and continuing challenges

Bailey

Gilkerson

Browning

2013

Veterinary Microbiology

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“…ERVA vaccines have been shown to aid in the reduction of the incidence and severity of diarrhea and also in the intensity and duration of viral shedding, however they do not guarantee full protection [1,21,22]. In addition, previous studies have shown that there is significant antigenic variation among ERVA genotypes, which leads to emergence of viruses that are not neutralized by antibodies elicited by the current vaccines [24][25][26][27][28][29]. Moreover, temporal and spatial variations in the prevalence and distribution of ERVA genotypes has been previously reported [2,29,30].…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples

Carossino

Barrandeguy

Erol

et al. 2019

Virol J

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Background Equine rotavirus A (ERVA) is the leading cause of diarrhea in neonatal foals and has a negative impact on equine breeding enterprises worldwide. Among ERVA strains infecting foals, the genotypes G3P[12] and G14P[12] are the most prevalent, while infections by strains with other genomic arrangements are infrequent. The identification of circulating strains of ERVA is critical for diagnostic and surveillance purposes, as well as to understand their molecular epidemiology. Current genotyping methods available for ERVA and rotaviruses affecting other animal species rely on Sanger sequencing and are significantly time-consuming, costly and labor intensive. Here, we developed the first one-step multiplex TaqMan ® real-time reverse transcription polymerase chain reaction (RT-qPCR) assay targeting the NSP3 and VP7 genes of ERVA G3 and G14 genotypes for the rapid detection and G-typing directly from fecal specimens. Methods A one-step multiplex TaqMan ® RT-qPCR assay targeting the NSP3 and VP7 genes of ERVA G3 and G14 genotypes was designed. The analytical sensitivity was assessed using serial dilutions of in vitro transcribed RNA containing the target sequences while the analytical specificity was determined using RNA and DNA derived from a panel of group A rotaviruses along with other equine viruses and bacteria. The clinical performance of this multiplex assay was evaluated using a panel of 177 fecal samples and compared to a VP7-specific standard RT-PCR assay and Sanger sequencing. Limits of detection (LOD), sensitivity, specificity, and agreement were determined. Results The multiplex G3 and G14 VP7 assays demonstrated high specificity and efficiency, with perfect linearity. A 100-fold difference in their analytical sensitivity was observed when compared to the singleplex assays; however, this difference did not have an impact on the clinical performance. Clinical performance of the multiplex RT-qPCR assay demonstrated that this assay had a high sensitivity/specificity for every target (100% for NSP3, > 90% for G3 VP7 and > 99% for G14 VP7, respectively) and high overall agreement (> 98%) compared to conventional RT-PCR and sequencing. Conclusions This new multiplex RT-qPCR assay constitutes a useful, very reliable tool that could significantly aid in the rapid detection and G-typing of ERVA strains circulating in the field.

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“…were untypable . Hardy et al (1991) found aU of the isolates they analyzed were of 03 serotype. In pigs, rotaviruses with 04 and 05 predominate (Zaberezhny et al, 1991;Pauletal., 1992a).…”

Section: Rotavirusesmentioning

confidence: 98%

“…Recent data indicates that rotaviruses with additional O and P types may be present in calves (Mummidi et al, 1992). In horses, the 03 serotype is most predominant : Hardy et al, 1991. Out of 70 equine rotavirus isolates tested 63% were serotype 03.…”

Section: Rotavirusesmentioning

confidence: 99%

Expression and characterization of bovine rotavirus proteins in the baculovirus system

Lyoo

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INFORMATION TO USERSThis manuscript has been reproduced from the microfihn master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter fece, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.

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Analysis of serotypes and electropherotypes of equine rotaviruses isolated in the United States

Cited by 27 publications

References 16 publications

Equine rotaviruses—Current understanding and continuing challenges

Equine rotaviruses—Current understanding and continuing challenges

Development and evaluation of a one-step multiplex real-time TaqMan® RT-qPCR assay for the detection and genotyping of equine G3 and G14 rotaviruses in fecal samples

Expression and characterization of bovine rotavirus proteins in the baculovirus system

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