Analysis of sensitivity of stromal stem cells (CFU-f) from rat bone marrow and fetal liver to 5-fluorouracil

Payushina, O. V.; Domaratskaya, E. I.; Bueverova, E. I.; Nikonova, T. M.; Буторина, Н. Н.; Молчанова, Е. А.; Старостин, В. И.

doi:10.1134/s1062359006060021

Cited by 5 publications

(7 citation statements)

References 17 publications

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“…Thus, in this study, we confirmed the previously obtained data on the existence in the bone marrow and fetal liver of two subpopulations of MSCs differing in sensitivity to the cytotoxic effects of 5 FU Payushina et al, 2006). Regardless of the level of sensitivity to 5 FU, MSCs from both organs had the capability of adipogenesis; however, they rap idly lost it during passaging.…”

Section: Spontaneous Ap Expression In the Primary Culturesupporting

confidence: 91%

“…Previous studies Payushina et al, 2006) showed that some CFU F in the bone marrow and fetal liver were resistant to 5 FU in vitro and, in the case of bone marrow, in vivo, when the drug was injected into animals. The injection of 5 FU into pregnant females results in the death of the vast major ity of CFU F in the liver of fetuses, probably due to its high embryotoxicity.…”

Section: Spontaneous Ap Expression In the Primary Culturementioning

confidence: 98%

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Comparative analysis of subpopulations of mesenchymal stromal cells of the bone marrow and fetal liver differing in sensitivity to 5-fluorouracil

Payushina

Буторина

Sheveleva

et al. 2015

Biol Bull Russ Acad Sci

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It is shown that the effect of 5 fluorouracil (5 FU) on mesenchymal stromal cells (MSCs) of the bone marrow and fetal liver of the rat enriches the population with cells with a reduced proliferative potential. It is shown that, in the course of passaging, the 5 FU resistant MSCs of the bone marrow and fetal liver lose their ability to differentiate in the osteogenic direction more and less rapidly, respectively, than the 5 FU sen sitive MSCs.

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Section: Spontaneous Ap Expression In the Primary Culturesupporting

confidence: 91%

Section: Spontaneous Ap Expression In the Primary Culturementioning

confidence: 98%

Comparative analysis of subpopulations of mesenchymal stromal cells of the bone marrow and fetal liver differing in sensitivity to 5-fluorouracil

Payushina

Буторина

Sheveleva

et al. 2015

Biol Bull Russ Acad Sci

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“…The chemotherapeutic agent, 5-Fu, is a pyrimidine analogue that inhibits thymidylate synthase, a necessary synthesis enzyme for DNA synthesis. 5-Fu specifically targets proliferating cells, and G0 phase cells can be spared due to 5-Fu induced cell death [ 28 , 29 ]. Thus, theoretically and practically, it is reasonable to select and enrich stem cells by 5-Fu treatment [ 23 , 28 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Expression and Role of Oct3/4 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment

Jia

et al. 2017

BioMed Research International

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Objective We aimed to investigate how the embryonic stem cell-related gene Oct3/4 changes during the injury-repair process of distal pulmonary epithelium induced by 5-fluorouracil (5-Fu). Methods We have developed the lung injury model induced by 5-Fu and observed the dynamic changes of Oct3/4 by indirect immunofluorescence, Western blot, and quantitative real-time PCR. Immunofluorescence double staining was used to compare the positions of Oct3/4(+) cells and other reported alveolar epithelial stem cells. Results Oct3/4(+) cells were not found in normal rat lung epithelial cells. However, after treatment with 5-Fu, Oct3/4(+) cells appeared at 12 h, reached the peak at 24 h, then decreased at 48 h, and eventually disappeared at 72 h. Oct3/4 was localized in the nucleus. We found that the sites of Clara cell secretory protein and surfactant protein-C dual positive cells were apparently different from Oct3/4(+) cells. Conclusions Our results revealed that, in rat alveolar epithelium, expression of Oct3/4 could be induced after treatment with 5-Fu, then decreased gradually, and was silenced following the alveolar epithelial differentiation. We hold that Oct3/4(+) cells are lung stem cells, which can provide new evidence for identification and isolation of lung epithelial stem cells.

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“…A regeneration model of ex vivo trachea damaged by 5-FU has been constructed by us previously (30). 5-FU is a member of the antimetabolite drug family and after treatment with 5-FU, normally proliferating tracheal epithelium desquamated, leaving only a few cells in G0 phase at the basement membrane (38)(39)(40).These cells were PCNA negative, indicating that they were in G0. In this study, we have repeatedly adjusted the concentration of 5-FU to maintain integrity of the basement membrane, this was indicated by silver staining (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Expression and role of Oct3/4, Nanog and Sox2 in regeneration of rat tracheal epithelium

Song¹,

Jia²,

Jia³

et al. 2009

Cell Proliferation

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Analysis of sensitivity of stromal stem cells (CFU-f) from rat bone marrow and fetal liver to 5-fluorouracil

Cited by 5 publications

References 17 publications

Comparative analysis of subpopulations of mesenchymal stromal cells of the bone marrow and fetal liver differing in sensitivity to 5-fluorouracil

Comparative analysis of subpopulations of mesenchymal stromal cells of the bone marrow and fetal liver differing in sensitivity to 5-fluorouracil

Expression and Role of Oct3/4 in Injury-Repair Process of Rat Alveolar Epithelium after 5-Fu Treatment

Expression and role of Oct3/4, Nanog and Sox2 in regeneration of rat tracheal epithelium

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