Analysis of selected transcript levels in porcine spermatozoa, oocytes, zygotes and two-cell stage embryos

Kempisty, Bartosz; Antosik, Paweł; Bukowska, Dorota; Jackowska, M.; Lianeri, Margarita; Jaśkowski, J. M.; Jagodzińśki, Paweł P.

doi:10.1071/rd07211

Cited by 46 publications

(42 citation statements)

References 47 publications

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“…They suggested that these spermatozoal mRNAs reflect a toxic effect during early embryogenesis. These results were confirmed by Kempisty et al (2008) in the porcine oocyte model. They detected the presence of spermatozoal mRNA encoding PRM2 and Clu in porcine zygotes and two-cell stage embryos.…”

Section: Mammalian Coc Maturationsupporting

confidence: 67%

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Kempisty¹,

Bukowska²,

Piotrowska³

et al. 2011

Vet. Med. - Czech

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ABSTRACT:Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as well as appropriate implantation. Since in some species of mammals the efficiency of IVM maturation is still very low, many studies have focused on new combinations of media supplements. In some species of mammals, including pigs, mice, bovines, goats and dogs, EGF, BSA, and progesterone are successfully used as an enhancer of the IVC system. Application of the Lab-on-Chip system in reproductive biology opens new possibilities for the development of techniques available for the assessment of the developmental competency or potency manifested by mammalian oocytes and embryos. In most cases, the Lab-on-Chip system was used in mice. However, an increasing number of examples are available in which the system has been applied to evaluate porcine and bovine gametes and embryos. In this article, the role of EGF, BSA and progesterone is described in relation to maturation efficiency of mammalian oocytes. Moreover, the possibilities of using microfluidics (Lab-on-Chip) for the detection of oocyte developmental competency are also shown.

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Section: Mammalian Coc Maturationsupporting

confidence: 67%

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Kempisty¹,

Bukowska²,

Piotrowska³

et al. 2011

Vet. Med. - Czech

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“…For example, Jasko et al (1990a) and findings provided a better concept of the biological importance of sperm RNAs, allowing the identification of biomarkers of stallion fertility. The sperm transcriptome has been studied in humans (Ostermeier et al 2002), boars (Kempisty et al 2008;Yang et al 2009) and bulls (Gilbert et al 2007;Bissonnette et al 2009;Feugang et al 2010), and the utility of sperm RNA as a marker of infertility has been explored (Miller 2000;Steger 2001), whereby differences in transcript levels in spermatozoa with different motilities (Bissonnette et al 2009;Lambard et al 2004), as well as between normal and abnormal sperm samples (Platts et al 2007;Steger et al 2003), have been reported. Krawetz (2005) demonstrated that some of the sperm RNAs can be delivered to the oocyte during fertilisation.…”

Section: Introductionmentioning

confidence: 99%

Implication of transcriptome profiling of spermatozoa for stallion fertility

Suliman

Becker

Wimmers

2018

Reprod. Fertil. Dev.

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Abstract. Poor fertility of breeding stallions is a recognised problem in the equine industry. The aim of the present study was to detect molecular pathways using two groups of stallions that differed in pregnancy rates as well as in the proportion of normal and motile spermatozoa. RNA was isolated from spermatozoa of each stallion and microarray data were analysed to obtain a list of genes for which transcript abundance differed between the groups (P #0.05, fold change $1.2). In all, there were 437 differentially expressed (DE) genes between the two groups (P # 0.05, fold change $1.2). Next, the DE genes were analysed using Database for Annotation, Visualisation, and Integrated Discovery (DAVID). Finally, ingenuity pathways analysis (IPA) was used to identify top biological functions and significant canonical pathways associated with the DE genes. Analysis using the DAVID database showed significant enrichment in the gene ontology (GO) term 'RNA binding' (P ¼ 0.05) and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cytokinecytokine receptor interaction (P ¼ 0.02). Furthermore, IPA analysis showed interconnected biological functions and canonical pathways involved in the regulation of spermatogenesis and male fertility. In addition, significantly enriched metabolic pathways were identified. In conclusion, the present study has identified, for the first time, molecular processes in stallion spermatozoa that could be associated with stallion fertility.

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“…56: [41][42][43][44][45][46][47][48] 2010) ver the past few years, a number of mammalian genes predominantly or exclusively expressed in germ cells have been discovered. Functional studies have reported the essential role of these genes during gametogenesis, folliculogenesis and early embryonic development [1][2][3][4][5][6][7]. NLRP5 (NLR, pyrin domain containing5), also called MATER (Maternal Antigen That Embryos Require), is one of these genes.…”

mentioning

confidence: 99%

Characterization of Maternal Antigen That Embryos Require (MATER/NLRP5) Gene and Protein in Pig Somatic Tissues and Germ Cells

Pisani

Ramelli

Lazzari

et al. 2010

Journal of Reproduction and Development

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Abstract. Maternal effect genes produce mRNA or proteins that accumulate in the egg during oogenesis and control the developmental program until embryonic genome activation takes place. NLRP5 (NLR family, Pyrin domain containing 5), also called MATER (Maternal Antigen That Embryos Require) is one of the genes required for normal early embryonic development, although its precise function remains to be elucidated. The aim of the present study was to analyze the NLRP5 gene expression pattern and protein distribution in somatic tissues and germ cells in the pig. Reverse transcription was performed on mRNA from germinal vescicle (GV) oocytes and total RNA from spermatozoa and tissues from different organs. The transcript for NLRP5 gene was identified only in ovaries and oocytes. The presence of NLRP5 protein was detected only in ovaries by western blot analysis and immunohistochemistry. Key words: Maternal effect genes, NLR family, Pyrin domain containing 5 (NLRP5), Oocytes, Pig (J. Reprod. Dev. 56: [41][42][43][44][45][46][47][48] 2010) ver the past few years, a number of mammalian genes predominantly or exclusively expressed in germ cells have been discovered. Functional studies have reported the essential role of these genes during gametogenesis, folliculogenesis and early embryonic development [1][2][3][4][5][6][7]. NLRP5 (NLR, pyrin domain containing5), also called MATER (Maternal Antigen That Embryos Require), is one of these genes. However, its precise function remains to be elucidated, although the transcription patterns may suggest a role in embryonic genome activation.Maternal mRNAs accumulated in the oocyte have a crucial role for successful embryo development before activation of the embryonic genome (EGA) [3], and some of them are also required for embryo development after EGA and implantation. These genes, which are called maternal effect genes and which include NLRP5, Zygotic Arrest 1 (Zar1) [8,9], Stella [10,11] and Nucleoplasmin 2 (Npm2) [12], are all required for normal embryonic development beyond the 1-and 2-cell stage in mice [13,14]. Knock-out of these genes leads to incapacity of the embryo to develop beyond the 2-cell stage [15].Developmental block in embryos produced in vitro remains the main problem in assisted reproduction of farm animals, particularly in cattle and pigs. An increasing amount of data indicates that the embryonic genome activation is crucial for the success of preimplantation embryo development [16]. To ensure maternal-embryo transition (MET) of gene expression, oocytes have to reach a sufficiently advanced level of developmental competence during differentiation and maturation [17][18][19]. Maternally expressed genes are implicated in this process, and some of them are associated with developmental competence [20,21]. In domestic species, MET occurs at the 4-16-cell stage [22], a stage in which enough biological material is available to study the fate of maternal mRNAs and their action in regulating embryo cleavages. Studying these genes in farm animals provides a model ...

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Analysis of selected transcript levels in porcine spermatozoa, oocytes, zygotes and two-cell stage embryos

Cited by 46 publications

References 47 publications

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Implication of transcriptome profiling of spermatozoa for stallion fertility

Characterization of Maternal Antigen That Embryos Require (MATER/NLRP5) Gene and Protein in Pig Somatic Tissues and Germ Cells

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