Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection

Lau, Aik Jiang; Woo, Soo-On; Koh, Hwee‐Ling

doi:10.1016/s0021-9673(03)01135-x

Cited by 146 publications

(108 citation statements)

References 15 publications

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“…4. By attentive study of the mass spectra of these compounds and comparison with standards and reference data (Hu et al, 2005;Lau et al, 2003;Li et al, 2005c;Xiao et al, 2004;Zhang and Cheng, 2006;Zhang et al, 2005a), a total of 36 major chromatographic peaks including 14 saponins, 13 phenolic acids and nine diterpenoid quinones were designated and identified. The HPLC-MS data and the relative amount based on total ion current chromatogram are metabolic changes in vivo and chemical constituent profile in biological systems.…”

Section: Analysis Of Chemical Components In Fdp By Hplc-msmentioning

confidence: 99%

“…For instance, HPLC-MS method was used for qualitative identification of 11 phenolic acids in Danshen injection (Zhang et al, 2005a), and LC-MS-MS method for the identification of thirteen phenolic acids and five diterpenoid quinones in Danshen (Hu et al, 2005). As for Sanqi, LC-MS method was also developed for qualitative identification of six to eight saponins in Sanqi and 19 saponins in combined preparation containing Danshen and Sanqi (Lau et al, 2003;Li et al, 2005c;Xiao et al, 2004;Zhang and Cheng, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng by LC‐ESI‐MS methods

Wei

Shu

et al. 2007

Biomedical Chromatography

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High-performance liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) methods were developed for the analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng (commonly known as Fufang Danshen prescription, FDP). The HPLC experiments used a reversed-phase Zorbax C(18) column with the column temperature at 30 degrees C and a binary mobile phase system consisting of aqueous formic acid (0.1%, v/v) and acetonitrile using a gradient elution at the flow rate of 1.0 mL/min. The ESI-MS was operated with a single-quadrupole mass spectrometer in both negative and positive ion modes. 36 major chromatographic peaks of FDP, including 14 saponins, 13 phenolic acids and nine diterpenoid quinones were characterized by their MS spectra and in comparison with some of the reference standards. In addition, after oral administration of extraction of FDP, the rat's plasma, urine and feces were also analyzed; 53 metabolic components including 30 original components and 23 transformative components of FDP were detected, and possible metabolic pathways of some components in FDP were given. The analysis of chemical and metabolic components in FDP by HPLC-MS methods could be a useful means of identifying the multi-components of FDP and to hint at their possible metabolic mechanism of action in the body.

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Section: Analysis Of Chemical Components In Fdp By Hplc-msmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng by LC‐ESI‐MS methods

Wei

Shu

et al. 2007

Biomedical Chromatography

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“…Discussion. ± The dammarane-type saponins from the roots of Panax notoginseng, which include ginsenosides and notoginsenosides, account for 12% of the total root dry weight, and are composed of protopanaxadiol and protopanaxatriol glycosides [12] [13]. Ginsenoside Rd (1) was considered to be one of four major components found in the total saponins from the roots of P. notoginseng, accounting for ca.…”

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confidence: 99%

Ginsenoside Rd from Panax notoginseng Is Cytotoxic towards HeLa Cancer Cells and Induces Apoptosis

Yang

Sun

2006

Chemistry & Biodiversity

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The saponin ginsenoside Rd (1), isolated from Panax notoginseng, is used for the treatment of cardiovascular diseases, inflammation, different body pains, trauma, and internal and external bleeding due to injury. In this study, we report that 1 inhibits the cell growth of human cervical cancer (HeLa) cells in a concentration- and time-dependent manner, with an IC(50) value of 150.5+/-0.8 mcirog/ml after 48 h of incubation. The drug-treated cells displayed features of apoptosis, including typical morphological characteristics and formation of DNA ladders, as evident from agarose-gel electrophoresis. Flow-cytometric analysis showed that the cell-cycle distribution of HeLa cells exposed to 1 is characterized by a decrease of the G(0)/G(1)-phase and an increase of the S-phase cells, respectively, in a dose-dependent manner. The apoptotic rate of HeLa cells treated for 48 h with 210 microg/ml of 1 was 35.8%. Further, 1 was found to increase the expression of Bax and to decrease the expression of Bcl-2 proteins, respectively, and to lower the mitochondrial transmembrane potential of HeLa cells. The caspase-3 inhibitor DEVD-CHO (at 2 microM) increased the viability of HeLa cells treated with 1. Taken together, our study suggests that ginsenoside Rd (1) significantly inhibits HeLa cell proliferation, and induces cell apoptosis through down-regulating Bcl-2 expression, up-regulating Bax expression, lowering the mitochondrial transmembrane potential, and activating the caspase-3 pathway. Thus, 1 could serve as a lead to develop novel chemotherapeutic or chemopreventive agents against human cervical cancer.

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“…notoginseng contains ~12% total saponins. The three major types of saponins are ginsenosides (e.g., Rb1, Rg1, Rg3), notoginsenosides (e.g., R1, R2, R3) and jiaogulan glucosides (e.g., RVI) (30)(31)(32)(33). The structures of both notoginsenosides and ginsenosides exhibit a tetracyclic gonane steroid core.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng

Lee

Hsieh

et al. 2016

Oncology Reports

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Abstract. Panax notoginseng (P. notoginseng) and its components are used as traditional Chinese medicine for cardiovascular disease, although studies concerning the anti-metastatic properties of these compounds are limited. The goal of this study was to investigate the effects of notoginsenoside R1 (NGR1), an important compound derived from P. notoginseng, on the metastasis of human colorectal cancer (CRC). The migratory, invasive, and adhesive abilities of cultured human CRC cells (HCT-116) treated with NGR1 and expression of metastasis-associated regulatory molecules were assessed. The migratory and invasive abilities of the HCT-116 cells were reduced after treatment with 75, 150 or 300 µM NGR1 for 24 h. When HCT-116 cells were incubated with 150 or 300 µM NGR1 for 24 h, matrix metalloproteinase (MMP)-9 expression was reduced compared with that of the control group. In the adhesion reaction assays, treatment with 150 or 300 µM NGR1 led to significantly decreased adhesion of the HCT-116 cells to endothelial cells (EA.hy926 cells). Levels of integrin-1 protein were significantly decreased in the HCT-116 cells following treatment with 75, 150 or 300 µM NGR1, and levels of E-selectin and intercellular adhesion molecule 1 (ICAM-1) proteins were significantly decreased in the EA.hy926 cells treated with 75, 150 or 300 µM NGR1. Scanning electron microscopy examination indicated that HCT-116 cells treated with lipopolysaccharide (LPS) combined with 300 µM NGR1 exhibited a less flattened and retracted shape compared with cells treated with LPS alone, and this change in shape is characteristic of extravasation. Additionally, the transepithelial electrical resistance of the EA.hy926 endothelial cell monolayer increased after incubation with 150 or 300 µM NGR1 for 24 h. Overall, these results demonstrated the anti-metastatic properties of 150 or 300 µM NGR1, a compound that affects CRC metastasis by inhibiting cell migration, invasion, and adhesion and by regulating expression of metastasis-associated signalling molecules.

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Analysis of saponins in raw and steamed Panax notoginseng using high-performance liquid chromatography with diode array detection

Cited by 146 publications

References 15 publications

Analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng by LC‐ESI‐MS methods

Analysis of chemical and metabolic components in traditional Chinese medicinal combined prescription containing Radix Salvia miltiorrhiza and Radix Panax notoginseng by LC‐ESI‐MS methods

Ginsenoside Rd from Panax notoginseng Is Cytotoxic towards HeLa Cancer Cells and Induces Apoptosis

Inhibition of human colorectal cancer metastasis by notoginsenoside R1, an important compound from Panax notoginseng

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