Analysis of Rye Pollen &lt;i&gt;(Secale cereale)&lt;/i&gt; Allergens Using Patients’ IgE, Immunoprint, Western Blot and Monoclonal Antibodies

Westphal, W.; Becker, Wolf‐Meinhard; Schlaak, M

doi:10.1159/000234608

Cited by 14 publications

(9 citation statements)

References 22 publications

(43 reference statements)

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“…Studies performed with murine monoclonal antibodies also demonstrated the strong cross-reactivity between protein extracts made from S. cereale, but also Festuca rubra, L, perenne, P. pratensis, P. pratense, D. glomerata and A. odoratum [15]. These results confirm the high antigenic similarity between these species, as can be anticipated from their close phylogenetic relationship.…”

Section: Molecular Immunologysupporting

confidence: 73%

Cross‐reactivity between pollen allergens from common Pooideae grasses and rye

Hrabina

Sieber²,

Gouyon

et al. 2008

Clin Exp All Rev

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Summary In Germany, specific pollen extracts are currently used for immunotherapy of rye (Secale cereale) allergy. Like most common grasses, S. cereale belongs to the Pooideae subfamily. Using sera from grass pollen‐allergic patients, immunoblotting and ELISA inhibition studies demonstrate that a five‐grass allergenic extract from Anthoxanthum odoratum, Dactylis glomerata, Lolium perenne, Poa pratensis and Phleum pratense inhibits over 90% of IgE binding to rye allergens. This result confirms the high degree of homology between allergens from S. cereale and common grass species. We conclude that a five‐grass pollen mixture is appropriate for desensitization of patients who are allergic to rye pollen without the need for additional rye pollen extracts.

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Section: Molecular Immunologysupporting

confidence: 73%

Cross‐reactivity between pollen allergens from common Pooideae grasses and rye

Hrabina

Sieber²,

Gouyon

et al. 2008

Clin Exp All Rev

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“…The NC was blocked with 0-1 "/o Tween. cut in strips and incubated with patient sera as described by Westphal [9]. For detection ofspecific IgE and IgG, an alkaline phosphatase linked 2nd goat-anti-human antibody (DIANOVA) was used.…”

Section: Treatmentmentioning

confidence: 99%

IgE, IgG, IgG1 and IgG4 patterns in yellow jacket allergic patients during immunotherapy with a venom depot extract

Jeep

Meysel

Kunkel

1992

Clin Experimental Allergy

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Using immunoblot studies, detailed antibody patterns were performed with the sera of 10 yellow jacket allergic patients undergoing specific immunotherapy with a yellow jacket venom SQ-depot extract (ALK, Denmark). Five males and five females (age range 10-65, mean 48 years) were investigated. All patients had a history of systemic reactions after an insect sting and a positive skin-prick test at a dose of at least 100 micrograms/ml yellow jacket venom. Yellow jacket venom (ALK) was separated on a 7.5-20% SDS-PAGE, transferred to nitrocellulose (NC), and then the NC-strips were incubated with the patients' sera. For detection of IgE, IgG, IgG1 and IgG4, an alkaline phosphatase linked 2nd antibody was used. Prior to immunotherapy, a strong IgE binding was detected at 25 kD in nine of 10 patients, representing Antigen-5 (Ag-5) as major allergen. Reactivity to this antigen was also present with the other immunoglobulin classes, although not as marked. In addition, a second frequent IgG and IgG1-binding band was seen at 35 kD (phospholipase A). Only weak or no binding to this band was found with IgE and IgG4. Binding to hyaluronidase (43 kD) was seen only in three cases. During immunotherapy, a significant increase in IgG and particularly IgG4 staining was found with Ag-5, whereas hyaluronidase induces mainly an IgG1 response and phospholipase A showed only a weak IgG response. In addition, the formation of new IgG4 binding to proteins in the region of about 70-90 kD was found in most patients. The dose necessary for the induction of this antibody formation was greater than or equal to 150.00 SQ yellow jacket venom.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…These allergenic determinants are the sites to which the IgE antibody is produced. More precise information concerning the clinical relevance of these al lergenic determinants may be obtained by using purified proteins [9][10][11],…”

Section: Introductionmentioning

confidence: 99%

Immunodetection and Comparison of Melaleuca, Bottlebrush, and Bahia Pollens

Sweeney

Hosseiny²,

Hunter³

et al. 1994

Int Arch Allergy Immunol

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Pollen extracts of two trees, Callistemon citrinis (bottlebrush) and Melaleuca leucadendron (melaleuca), as well as the grass Paspalum notatum (bahia) were analyzed for antigenic and allergenic cross-reactivity using SDS-PAGE and Western blotting. SDS-PAGE analysis of all three pollen extracts revealed multiple antigenic components which were reactive with rabbit antisera made to each pollen extract. Comparison of reduced and nonreduced mobility patterns suggested the possible presence of multichain proteins linked by disulfide bonds. Clinical studies demonstrated that 81% of the patients skin test positive to at least one of the pollens were also positive to the other two. Sixty-three percent of allergic individuals studied showed a high correlation between skin test results and the number of IgE-binding components analyzed by immunoblotting. These IgE-reactive components were detected in the molecular weight range of 29–66 kD. Western blot analysis detected more IgE-binding components in bahia pollen extracts as compared with the tree pollens, consistent with other reports of grass pollens being more allergenic than those from trees and weeds. Each patient’s serum had a unique IgE-binding pattern, indicating heterogeneity of immune response; however, common major determinants were detected by a large percentage of the allergic patient’s sera.

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Analysis of Rye Pollen <i>(Secale cereale)</i> Allergens Using Patients’ IgE, Immunoprint, Western Blot and Monoclonal Antibodies

Cited by 14 publications

References 22 publications

Cross‐reactivity between pollen allergens from common Pooideae grasses and rye

Cross‐reactivity between pollen allergens from common Pooideae grasses and rye

IgE, IgG, IgG1 and IgG4 patterns in yellow jacket allergic patients during immunotherapy with a venom depot extract

Immunodetection and Comparison of Melaleuca, Bottlebrush, and Bahia Pollens

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