Analysis of rotational flow generated by circular motion of an end effector for 3D micromanipulation

Abstract

This paper presents a non-contact manipulation method using rotational flow generated by high speed motion of a glass needle. In order to generate an applicable motion that can precisely control the speed and position of a target object around an end effector, a manipulator actuated by three piezoelectric actuators is proposed. A compact parallel link produces precise three-dimensional motions. To reach the required end-effector frequency and amplitude, which is necessary for generating the rotational flow that can make rotational movement of microbeads having patterned data, the end effector motion is generated by controlling the three piezoelectric actuators at high speed. In this paper, we focus on rotational flow created by two-dimensional circular motion of the end effector mounted on the parallel link. By changing the amplitude and frequency of the circular motion, the generated swirl flow is analyzed in 3D space using 9.6-μm microbeads. We analyzed the velocity toward to the root of the end effector and angular velocity around the end effector by formulating models. From the analyzed data, we verified that the rotational flow has potential to manipulate micro-objects precisely in 3D space.

A Review of Single‐Cell Pose Adjustment and Puncture

“…Optical field [36,[103][104][105][106][107]109,115,167] Trapping D, ξ, δ Medium, High 4-20 High (0.0001 % 0.001) High rotation accuracy, label-free, single-cell, low drift Optical damage, expensive instrumentation, weak capture low throughput, bulky optical system with opaque Magnetic field [8,119,120,125,126,128,133,134,168] Magnetic gradient D, χ High 5-120 Higher (0.0001 % 0.01) Greater drive power, reliability and efficiency Constrained by the magnetic field distribution, requires pretreatment of cells, labeling, force hysteresis Acoustic field [138,139,[141][142][143][144][145][146]155,169] Axial acoustic forces D, ρ, β Low 10-3100 Low (1 % 10) Label-free Stimulation of cells, required piezoelectric substrates for chip fabrication, limited precise cell rotation Low cost, massive, article rotation Hydrodynamic drive [64,156,160,[160][161][162][163][164][165] Hydrodynamic inertial forces D Low 10-5 50 Low (1 % 10) Easy to integrate manufacturing Rotational accuracy, complex and cumbersome external control equipment, poor flexibility, difficult to control specific single-cell a)…”

“…Ou et al [160] 2021 Three-microtube-induced cyclonic flow 1 35 mm s Cell 270 Cell manipulation Liu et al [163] 2018 Cantilever resonance 1 0.24-5.08 rad s Mouse egg cell 100 Biological manipulation Kim et al [165] 2017 Vibration-induced flow caused by circular vibration of a single pipette 1 0 -120 rad s Microspheres 9.6 Cell rotation…”

High-Speed Manipulation of Microobjects Using an Automated Two-Fingered Microhand for 3D Microassembly

“…At this time, a stronger local flow than before is necessary to release the target. Using experimental results and vortex theory, we formulated simple equations to analyze the flow velocity [37]. The amplitude can be estimated by calculating the velocity.…”

A Review on Microscopic Visual Servoing for Micromanipulation Systems: Applications in Micromanufacturing, Biological Injection, and Nanosensor Assembly

“…Micromanipulation or microassembly techniques refer to the processing or manipulation of tiny objects, which are generally between millimeters and nanometers in size. They are mainly used in biological engineering, microdevice processing or assembly, medical engineering and other fields of fine operation [1]. MEMS (microelectromechanical systems) [2,3], MOEMS (micro-optoelectromechanical systems) [4], BioMEMS (biological microelectromechanical systems) [5][6][7][8], and other similar microsystems are generated as a result of the multidisciplinary interactions between sensors, precision machining, biological engineering, microelectronics and precision measurement technologies.…”