Analysis of Romanian Bacteroides isolates for antibiotic resistance levels and the corresponding antibiotic resistance genes

Székely, Edit; Eitel, Zsuzsa; Molnár, Szabolcs; Szász, Izabella; Bilca, Doina; Sóki, Jozsef

doi:10.1016/j.anaerobe.2014.09.001

Cited by 21 publications

(10 citation statements)

References 13 publications

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“…According to studies on antimicrobial resistance with B. fragilis, rates of resistance to CLI in several countries are reported between 24% and 56% (KANGABA et al, 2015;KOUHSARI et al, 2019). On the other hand, there are few reports of resistance to PTZ and MET (SZÉKELY et al, 2015;YIM et al, 2015). In the present study, the clinical strain P34 was susceptible to the three tested antimicrobials, and the clinical strain P50 showed only resistant to clindamycin (MIC=1.024 μg/mL).…”

Section: Discussionmentioning

confidence: 42%

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

Nascimento¹,

Guedes²,

Bezerra³

et al. 2020

Pesquisa Científica E Tecnológica Em Microbiologia 3

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Section: Discussionmentioning

confidence: 42%

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

Nascimento¹,

Guedes²,

Bezerra³

et al. 2020

Pesquisa Científica E Tecnológica Em Microbiologia 3

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“…The presence of the ermF gene is often correlated with the tetracycline-resistance gene tet(Q). In tetracycline-resistant BFG strains, the tet(Q) gene induces resistance to tetracyclines in the bacterial ribosome [32]. In this study, the evaluated DNA extraction kits differed in their ability to detect ermF, linA and tet(Q) genes.…”

Section: Discussionmentioning

confidence: 97%

The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants

et al. 2018

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Wastewater treatment plants (WWTPs) are one of the main transmission sources of pathogenic bacteria and antibiotic-resistance genes in the natural environment. In this study, the presence of specific markers of Bacteroides fragilis group (BFG), B. dorei and genes encoding resistance to beta-lactams (cepA, cfxA), tetracyclines (tet(Q)), macrolides, lincosamides and streptogramins (MLS) mechanism (ermF, linA) was analyzed by standard PCR in the inflows and outflows from three wastewater treatment plants with the activated sludge process. Genetic material was isolated from wastewater samples with the use of two commercial kits for genomic DNA extraction, the Fast DNA SPIN Kit for Soil and the Genomic Micro AX Bacteria Gravity Kit. The quality of the isolated genetic material differed between the tested isolation kits. The Fast DNA SPIN Kit for Soil was more effective in detecting cfxA, ermF and linA genes. However, both extraction kits effectively identified tet(Q), bfr and HF183/BacR287 genes in all wastewater samples. The results of the study indicate that genes specific to BFG and B. dorei, and genes encoding resistance to MLS and tetracyclines are not completely eliminated during the wastewater treatment process.

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“…U bakterii beztlenowych, występujących w jelitach człowieka, powszechnie stwierdza się oporność na tetracykliny, erytromycynę, leki β-laktamowe czy fluorochinolony. Związane jest to z rozpowszechnieniem najbardziej typowych markerów oporności, do których zaliczyć można geny tet(Q), ermF, cepA, cfxA oraz cfiA [37,68] oraz mniej powszechne geny tet(X), tet(X1) i bexA [66]. Wexler [71] opisuje wzrost występowania u Bacteroides genu oporności na tetracyklinę tet(Q) z około 30% do ponad 80%, przy czym allele tet(Q) u różnych gatunków Bacteroides są w 96-100% identyczne na poziomie sekwencji DNA.…”

Section: Bakterie Z Rodzaju Bacteroides Jako Rezerwuar Determinantów unclassified

“…Obecnie, prawie wszystkie kliniczne izolaty Bacteroides spp. (> 80-90%) są tetracyklinooporne [3,66,71]. Jednym z mechanizmów tetracyklinooporności, opisywanym u B. fragilis, jest aktywne usuwanie leku z komórki bakterii za pomocą pomp tzw.…”

Section: Oporność Na śRodki Przeciwdrobnoustrojoweunclassified

“…genów cepA, cfxA, cifA czy też tet(X) kodujących oporność odpowiednio na penicyliny, cefalomycynę, karbapenemy i tetracykliny. W szczególnych przypadkach, tak jak to ma częściowo miejsce u Bacteroides sp., geny te mogę być uśpione, a ich aktywność jest zależna od występowania sekwencji insercyjnych [66]. W celu wykrycia genów oporności na antybiotyki stosuje się reakcje PCR i qPCR.…”

Section: Metody Określania Lekowrażliwościunclassified

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Bacteroides spp. – clinical significance, antibiotic resistance and identification methods

Niestępski

Harnisz

Korzeniewska

et al. 2017

Postępy Mikrobiologii - Advancements of Microbiology

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ujemne pałeczki, nie wytwarzające przetrwalników, które nie należały do Fusobacterium spp. czy Leptotrichia spp. Zmieniona systematyka Bacteroides i rodzajów spokrewnionych opiera się na analizach filogenetycznych sekwencji genu 16S rDNA i została przedstawiona w drugim wydaniu podręcznika "Bergey's Manual of Systematic Bacteriology" [32].Poniżej przedstawiono szczegółową systematykę rodzaju Bacteroides.

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Analysis of Romanian Bacteroides isolates for antibiotic resistance levels and the corresponding antibiotic resistance genes

Cited by 21 publications

References 13 publications

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants

Bacteroides spp. – clinical significance, antibiotic resistance and identification methods

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