Analysis of RNA Metabolism in Fission Yeast

Abstract

Here we focus on the biogenesis and function of messenger RNA (mRNA) in fission yeast cells. Following a general introduction that also briefly touches on other classes of RNA, we provide an overview of methods used to analyze mRNAs throughout their life cycles.

Introduction to Fission Yeast as a Model System

“…Although no alternative splicing has yet been observed in fission yeast, because of the high number of genes that contain introns, and the presence of genes encoding splicing factors such as SR-like proteins, it is possible that alternative splicing could occur in S. pombe (Awan et al 2013;Bitton et al 2014). For an overview of methods used to analyze mRNAs, including methods to investigate splicing, see Introduction: Analysis of RNA Metabolism in Fission Yeast (Wise and Nielsen 2016).…”