Analysis of rice blast resistance gene Pi-z in rice germplasm using pathogenicity assays and DNA markers

RoyChowdhury, Moytri; Jia, Yulin; Jackson, Aaron; Jia, Melissa H.; Fjellstrom, Robert G.; Cartwright, R. D.

doi:10.1007/s10681-011-0481-3

Cited by 46 publications

(28 citation statements)

References 21 publications

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“…The observed frequency fitted to the single-gene model. RoyChowdhury et al (2012) found Pi-b blast resistance gene in the national small grain collection and mentioned that RM208 marker is tightly linked to Pi-b gene. Tanweer et al (2015) cloned the Pi-b and Pi-kh genes from Malaysian local rice cultivar Pongsu Seribu 2, thus it is confirmed from their research that Pongsu Seribu 2 contain atleast two dominant genes.…”

Section: Discussionmentioning

confidence: 99%

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Tanweer

Rafii

Sijam

et al. 2015

Australasian Plant Pathol.

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The study was conducted to identify the suitable SSR markers in relation to blast disease of rice. Backcross breeding was applied and BC 2 F 1 generation was produced using rice variety Pongsu Seribu 2 as blast resistant parent and MR219 as susceptible parent. Eleven SSR markers related to blast resistance genes (Pi gene) were found highly polymorphic between the parental lines. According to single-gene model only two markers, RM208 and RM206, fitted to the expected test cross ratio (1:1) in BC 2 F 1 generation with the relation to resistant and susceptible plants. Further BC 2 F 1 population carrying 320 plants were inoculated with the highly virulent pathotype P7.2 of Magnaporthe oryze. Chi-square (χ2) test for single-gene model showed goodness of fit (p=0.45) to the expected segregation ratio (1:1) for phenotypic segregation in BC 2 F 1 population. In marker segregation analysis two markers RM208 (χ2=1.513, p=0.218) and RM206 (χ2=0.613, P=0.433) clearly produced goodness of fit to the expected test cross ratio (1:1) for the single-gene model. The present finding could help the rice breeders to monitor the blast resistance genes with appropriate and highly segregating SSR markers in rice varietal development program against blast disease.

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Section: Discussionmentioning

confidence: 99%

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Tanweer

Rafii

Sijam

et al. 2015

Australasian Plant Pathol.

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“…They identified five QTLs located on chromosomes 6, 7, 9 and 11. Chowdhury et al (2012) evaluated 111 germplasm accessions against rice blast resistance conferring gene Pi-z. Among the available germplasm accessions, 73 were found to carry the said gene.…”

Section: Significance Of Rice Germplasmmentioning

confidence: 99%

Molecular breeding of rice for improved disease resistance, a review

Khan

2015

Australasian Plant Pathol.

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Diseases are considered to be the major limiting factors in rice production around the globe. Considering rice as an important cereal crop, developing disease resistant cultivars is a prime objective of breeders. Compared to conventional breeding, molecular breeding especially using marker assisted selection appears to be more effective and precise. The best management of disease is to bring durable wide spectrum resistance in rice cultivars. This can be accomplished by accumulating both qualitative and quantitative resistance genes in to rice cultivars. Introducing these resistance genes from the wild relatives of rice in to commercial cultivars has greatly helped the breeders to accomplish this task. Marker assisted selection is extremely valuable in resolving the issues the breeders face with traditional breeding. A number of transgenic rice lines harboring the so called 'foreign' resistant genes have been produced. However such promising lines must be extensively replicated in fields to evaluate stable integration and continued expression of genes. None the less identification of disease resistance genes and quantitative trait loci (QTLs), use of marker assisted selection along with gene pyramiding and transgenic approaches all provide breeders a hope to build high yielding disease resistant cultivars. Based on such premises, we can truly envision broadening of our understanding the genetic and molecular basis of disease resistance in rice.

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“…492 genes have been cloned and used for crop protection [13]. However, the management of rice blast caused by P. oryzae through the use of disease resistant cultivars has become difficult because of the highly variable nature of the pathogen as well as favorable environmental conditions for infection during the crop season.…”

Section: Identification Of Rice Blast Resistance Genes In South Centrmentioning

confidence: 99%

Identification of Rice Blast Resistance Genes in South Central Coast of Vietnam Using Monogenic Lines under Field Condition and Pathogenicity Assays

Nguyen¹,

Truong²,

Nguyen³

et al. 2015

JAST-A

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Abstract:In the present study, the authors studied the interaction between 26 monogenic differentials carrying 26 blast resistance genes with 15 isolates of Pyricularia oryzae under field conditions and pathogenicity assays. The result in field conditions showed that area under disease progress curves (AUDPC) of monogenic lines was found to range from 0 to 142.3. Lijiangxintuanheigu (LTH) was susceptible in all of regions. The monogenic lines carrying Pik, Piz, Pi1, Pi7(t), Pi4(t), Pish, Pi9(t) and Pita were highly resistant to blast in all of regions. In plastic house net conditions, the percentage of virulent reactions of monogenic lines to 15 isolates was found to range from 23.1% to 84.6%. LTH was susceptible to all 15 isolates. All 26 monogenic lines were resistant to at least 3 isolates of P. oryzae, and the frequency of resistant reactions of the monogenic lines carrying Pik, Piz, Pi4(t), Pish, Pi1, Pi7(t), Pi9, Pik-m and Pita were 80.0%, 93.3%, 93.3%, 80.0%, 73.3%, 86.7%, 73.3%, 80.0%, 80.0%, 80.0% and 80.0%, respectively. These findings suggest that Pik, Piz, Pi4(t), Pish, Pi1, Pi7(t), Pi9, Pik-m and Pita may be important R genes for preventing blast disease in South Central Vietnam. Based on these data, a useful strategy for managing rice blast disease by stacking pyramiding blast R genes against pathogenic P. oryzae isolates at hotspot in South Central Coast of Vietnam was proposed.

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Analysis of rice blast resistance gene Pi-z in rice germplasm using pathogenicity assays and DNA markers

Cited by 46 publications

References 21 publications

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Molecular breeding of rice for improved disease resistance, a review

Identification of Rice Blast Resistance Genes in South Central Coast of Vietnam Using Monogenic Lines under Field Condition and Pathogenicity Assays

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