Analysis of rice Act1 5' region activity in transgenic rice plants.

Abstract

The 5' region of the rice actin 1 gene (Actl) has been developed as an efficient regulator of foreign gene expression in transgenic rice plants. To determine the pattern and level of rice Actl 5' region activity, transgenic rice plants containing the Actl 5' region fused to a bacterial 8-glucuronidase (Gus) coding sequence were generated. Two independent clonal lines of transgenic rice plants were analyzed in detail. Quantitative analysis showed that tissue from these transgenic rice plants have a level of GUS protein that represents as much as 3% of total soluble protein.We were able to demonstrate that Actl-Gus gene expression is constitutive throughout the sporophytic and gametophytic tissues of these transgenic rice plants. Plants from one transgenic line were analyzed for the segregation of GUS activity in pollen by in situ histochemical staining, and the inheritance and stability of Actl-Gos expression were assayed in subsequently derived progeny plants.

Molecular Cloning and Characterization of a Novel β-1,3-Glucanase Gene from Rice

“…As shown in Fig. 3, constitutive expression of Act1 20) was conˆrmed by this RT-PCR experiment, except for anthers at anthesis, which have a high content of actinˆlaments in germinating pollens. Osg1 transcripts were present in leaves, roots, and anthers.…”

Production and characterization of a large population of cDNA-overexpressing transgenic rice plants using Gateway-based full-length cDNA expression libraries

“…In this study, we constructed a Gateway-destination binary vector (pSMAHdN636L-GateA) carrying PAct-1 (Zhang et al 1991) in the T-DNA for ectopic overexpression of individual fl-cDNAs in rice, taking advantage of Gatewayentry clones and Gateway cloning technology (Fig. 1) to facilitate the production of rice fl-cDNA libraries.…”

“…3) with individual gene-specific primers (Supplemental Table 2). PAct-1 is a highly active promoter in both vegetative and reproductive tissues (Park et al 2010, Zhang et al 1991. Therefore, young leaves were used to examine the overexpression of introduced fl-cDNAs in the FOX lines.…”

Cis-regulatory elements used to control gene expression in plants

“…For example, the Act2 promoter, which is a modified Arabidopsis actin promoter, is substantially constitutive, but does not promote protein expression in hypocotyl, seed coat and microsporangia of Arabidopsis. Furthermore, the modified rice actin promoter is not active in the xylem of this plant (Zhang et al 1991;.…”