Analysis of Riboflavin and its Derivatives in Biologic Fluids and Tissues

Levels of folate, pyridoxine, niacin and riboflavin in traditional foods of Canadian Arctic Indigenous Peoples

“…Riboflavin can be determined by microbiological (Baker and Frank, 1975), direct fluorometric (AOAC, 2003), HPLC (Ang and Moseley, 1980) and capillary electrophoretic (Hustad et al, 1999) methods. Direct fluorescence and HPLC methods are most used for the assay of riboflavin in foods and the fluorescent method used after HPLC separation of riboflavin vitamers in the current study is more sensitive than UV detection and less subject to interference by other compounds present in muscle foods.…”

Multianalyte Quantification of Vitamin B6 and B2 Species in the Nanomolar Range in Human Plasma by Liquid Chromatography–Tandem Mass Spectrometry

“…Analytical techniques for the determination of vitamin B 2 concentrations include fluorometric (37 ), liquid chromatographic (38,39 ), and capillary electrophoretic methods (40 ). Most HPLC methods have been designed for the detection of high concentrations of vitamin B 2 in food, pharmaceutical preparations, and urine (38 ).…”

Serum Triiodothyronine Concentrations in Riboflavin-Deficient, Diabetic and Normal Mice

“…The results were expressed as activity coefficients (AC), calculated by dividing the amount of reduction of absorbance of NADPH in the presence of FAD for 10 min by the amount of reduction of absorbance of NADPH without added FAD for 10 min. AC values from 0.9 to 1.3 were considered normal, and those greater than 1.3 were regarded as evidence of riboflavin deficiency (8). Based upon AC values, both SA and KK mice were classified as normal (non-deficient) or riboflavin-deficient.…”

“…Although the mean T3 level was 30% higher in riboflavindeficient mice, the increase was not statisti- 49.00 f 6.00 (8) 58.00 f 5.00 (5) 77.00 k 17.00 (3) 69.00 f 13.00 (5) 49.00 k 9.00 (6) Significance ( cally significant because of the variability of the data.…”