Analysis of Repetitive DNA Sequences from Potato Cyst Nematodes and Their Use as Diagnostic Probes

Stratford, Rebecca

doi:10.1094/phyto-82-881

Cited by 21 publications

(11 citation statements)

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“…citri (61). Other potential targets for specific amplification include the short, interspersed repetitive elements present in bacteria (35,94), and the species-specific tandem DNA repeats in many eukaryotes, including potato cyst nematodes (139). A strain or isolate can also be marked, or transformed, with a known gene and primers specific for the reporter gene used to detect the isolate.…”

Section: < Zmentioning

confidence: 99%

The Polymerase Chain Reaction and Plant Disease Diagnosis

Henson¹

1993

Annual Review of Phytopathology

122

127

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Section: < Zmentioning

confidence: 99%

The Polymerase Chain Reaction and Plant Disease Diagnosis

Henson¹

1993

Annual Review of Phytopathology

122

127

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“…18 Each of these methods has problems for use in routine identification13 and none of them has been fully tested and validated against the range of field populations found in the UK, as most of the work has been done only on known species from glasshouse cultures. The PCR technique offers the prospect of a simple, rapid and reliable diagnostic tool for plant parasitic nematodes that will enable advisers to identify and quantify populations of PCN from field samples,16, 19, 20 as do ELISA‐based procedures 21…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales

Ibrahim

Minnis

Barker

et al. 2001

Pest Management Science

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Effective management of potato cyst nematodes (PCNs) requires simple, rapid and accurate identification and quantification of field populations. Soil samples from a survey of 484 fields in potato rotations in England and Wales were used to compare the identification and quantification of PCNs using IEF, PCR, ELISA and bait plant tests. The cyst counts and bait plant test revealed that 64.3% of field samples contained PCNs. Bait plant tests increased the detection rate of PCNs in field samples by 4-6.4%. This means that some infestations are cryptic and would not normally be detected by standard counts. IEF, PCR and ELISA methods distinguished between Globodera rostochiensis and G pallida and were able to register mixed populations; however they were not in full agreement. All methods suggested that G pallida is the dominant species in the field samples tested. The PCR results indicated that 66% of field samples contained pure G pallida, 8% contained pure G rostochiensis and 26% contained mixtures of the two species. Estimates of the relative process times taken per sample in the PCR, IEF and ELISA techniques are given.

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“…Physical detection methods, such as fork sampling, are slow and often ineffective. Molecular biology tools have been applied to the separation of PCN species and pathotypes (e.g., Schnick et al 1990;Stratford et al 1992;Folkertsma et al 1994). Marshall (1993) applied molecular dot-blot hybridisation to define the distribution of PCN species throughout New Zealand.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR)

Bulman

Marshall

1997

New Zealand Journal of Crop and Horticultural Science

107

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Molecular examination of the ribosomal internal transcribed spacer (ITS) region in potato cyst nematodes (PCN) is described. The ITS was amplified and sequenced from a number of PCN collections. A low level of sequence variation was found between Globodera rostochiensis, G. pallida, and a Peruvian PCN collection, but no variation within Australasian collections of species was noted. Polymerase chain reaction (PCR) primers based upon the G. rostochiensis-G. pallida sequence differences were designed and successfully used to identify mixed PCN species in a single PCR reaction.

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Analysis of Repetitive DNA Sequences from Potato Cyst Nematodes and Their Use as Diagnostic Probes

Cited by 21 publications

References 0 publications

The Polymerase Chain Reaction and Plant Disease Diagnosis

The Polymerase Chain Reaction and Plant Disease Diagnosis

Evaluation of PCR, IEF and ELISA techniques for the detection and identification of potato cyst nematodes from field soil samples in England and Wales

Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR)

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