Analysis of Relative Expression of the Hmga2 Gene and Oncogenic Microrna-221 in Cytological Slides Obtained by a Fine-Needle Aspiration Biopsy of the Thyroid Nodules

Титов, С. Е.; Иванов, М. Ф.; Tsivlikova, Elena V.; Ganzha, Maria; Малахина, Е. С.; Malek, Anastasia; Полоз, Т. Л.; Шевченко, С. П.; Колесников, Н. Н.

doi:10.17650/2313-805x-2017-4-4-24-31

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“…In our study, no tumors with overexpressed HMGA2 were diagnosed as benign in the histological analysis. This result is fairly consistent with our earlier findings where increased HMGA2 expression was not detected in any of 375 specimens cytologically diagnosed as goiters [36].…”

Section: Discussionsupporting

confidence: 93%

Combined quantitation of HMGA2 mRNA, microRNAs, and mitochondrial-DNA content enables the identification and typing of thyroid tumors in fine-needle aspiration smears

et al. 2019

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BackgroundAnalysis of molecular markers in addition to cytological analysis of fine-needle aspiration (FNA) samples is a promising way to improve the preoperative diagnosis of thyroid nodules. Nonetheless, in clinical practice, applications of existing diagnostic solutions based on the detection of somatic mutations or analysis of gene expression are limited by their high cost and difficulties with clinical interpretation.The aim of our work was to develop an algorithm for the differential diagnosis of thyroid nodules on the basis of a small set of molecular markers analyzed by real-time PCR.MethodsA total of 494 preoperative FNA samples of thyroid goiters and tumors from 232 patients with known histological reports were analyzed: goiter, 105 samples (50 patients); follicular adenoma, 101 (48); follicular carcinoma, 43 (28); Hürthle cell carcinoma, 25 (11); papillary carcinoma, 121 (56); follicular variant of papillary carcinoma, 80 (32); and medullary carcinoma, 19 (12). Total nucleic acids extracted from dried FNA smears were analyzed for five somatic point mutations and two translocations typical of thyroid tumors as well as for relative concentrations of HMGA2 mRNA and 13 microRNAs and the ratio of mitochondrial to nuclear DNA by real-time PCR. A decision tree–based algorithm was built to discriminate benign and malignant tumors and to type the thyroid cancer. Leave-p-out cross-validation with five partitions was performed to estimate prediction quality. A comparison of two independent samples by quantitative traits was carried out via the Mann–Whitney U test.ResultsA minimum set of markers was selected (levels of HMGA2 mRNA and miR-375, − 221, and -146b in combination with the mitochondrial-to-nuclear DNA ratio) and yielded highly accurate discrimination (sensitivity = 0.97; positive predictive value = 0.98) between goiters with benign tumors and malignant tumors and accurate typing of papillary, medullary, and Hürthle cell carcinomas. The results support an alternative classification of follicular tumors, which differs from the histological one.ConclusionsThe study shows the feasibility of the preoperative differential diagnosis of thyroid nodules using a panel of several molecular markers by a simple PCR-based method. Combining markers of different types increases the accuracy of classification.

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Section: Discussionsupporting

confidence: 93%