Analysis of regeneration protocols for micropropagation of Pterocarpus santalinus

Chakraborty, Tanushree; Chaitanya, K. V.; Akhtar, Nasim

doi:10.1007/s11816-021-00728-8

Cited by 4 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Long-established in vitro plants often demonstrate declined regenerative ability [108,109]. The reduced proliferation could be reversed in vitro following successive grafting onto vigorous rootstocks [19,33,106,110].…”

Section: Promotion Of Shoot Proliferationmentioning

confidence: 99%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

View full text Add to dashboard Cite

In vitro micrografting is an important technique supporting the micropropagation of a range of plant species, particularly woody plant species. Over the past several decades, in vitro micrografting has become a strategy to facilitate shoot recovery and acclimatization of in vitro-grown horticultural species. This review focuses on studies on horticultural crops over the past two decades that cover the establishment of in vitro micrografting, discusses factors affecting the success of in vitro micrografting, and provides commentary on the contribution of micrografting applications to the field of micropropagation. Considering the important roles of micrografting in the restoration of vigor and rooting competence, in promotion of shoot recovery following somatic embryogenesis and organogenesis, and in facilitation of shoot regrowth after cryopreservation, the potential use of this technique in facilitation of genetic engineering and safe conservation of horticultural species are specially highlighted.

show abstract

Section: Promotion Of Shoot Proliferationmentioning

confidence: 99%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Biotechnological methods may be valuable in the propagation and conservation of selected genotypes (Gaidamashvili and Benelli, 2021). In vitro propagation is not only less affected by the external environment but can also produce orderly and consistent young plants with ideal characteristics (Kumar et al, 2011;Martínez et al, 2021;Chakraborty et al, 2022). Adult trees are difficult to use as material because of the large number of endophytic bacteria, difficulty establishing an aseptic system, and shoot induction and differentiation, which dramatically limits in vitro propagation (Martínez et al, 2017a(Martínez et al, , 2021.…”

Section: Introductionmentioning

confidence: 99%

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

Zhang

Guo²,

Wu³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Castanopsis hystrix is one of the main timber trees grown in China. However, severe shortage of natural seeds and the difficulty of explant regeneration has limited seedling supply. As such, there is a need for research on asexual multiplication of C. hystrix. This study established a rapid propagation technology system for C. hystrix genotypes, including explant treatment, proliferation, and rooting. HZ (a modified MS medium) supplemented with 4.4 μM BA and 0.5 μM IBA was found to be the optimal medium for shoot sprouting. The maximum proliferation coefficient and the number of effective shoots was obtained on HZ medium supplemented with 2.6 μM BA and 1.0 μM IBA, were 3.00 and 5.63, respectively. A rooting rate of 83.33% was achieved using half-strength HZ medium supplemented with 3.2 μM NAA. Adding vitamin C (80 mg⋅l–1) for 7 days in a dark environment reduced the browning rate, while increasing the proliferation rate. Additionally, through cytological observation, we established how and where adventitious roots occur. The survival rate of transplanted plantlets was > 90%. This is the first report of an in vitro regeneration technique that uses stem segments of mature C. hystrix as explants.

show abstract

“…To our knowledge, there is no report of somatic embryogenesis in P. santalinus. Recently in vitro morphogenesis responses is reported from cotyledonary explants of immature zygotic embryos of P. santalinus (Chakraborty et al 2022a) following the analysis of regeneration protocols for micropropagation (Chakraborty et al 2022b). This study documents the regeneration of plantlets by establishment of somatic embryogenesis in red sandalwood and histological analysis to differentiate embryonic and non-embryonic cells from cotyledon explants.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis and Plantlet Regeneration in Red Sandalwood (Pterocarpus Santalinus)

Chakraborty

Chaitanya

Akhtar

2022

Preprint

Self Cite

View full text Add to dashboard Cite

Cotyledonary segments from the germinated immature zygotic embryo were used for somatic embryogenesis of red sandalwood (Pterocarpus santalinus). It was established on Murashige and Skoog (MS) medium containing 5% sucrose and amalgamation of 6-benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), and α-Naphthaleneacetic acid (NAA). All treatments were responsive for callus induction with the frequency range between 36–97%. The attempts to obtain embryogenesis with 3% sucrose were ineffective since only non-embryonic callus was observed. The maximum frequency of embryogenic induction (69.44%) was obtained in 0.1 mg/l BAP and 2 mg/l 2,4-D; the data was not significantly (p < 0.05) different from the result obtained when 0.1 mg/l BAP and 4 mg/l 2,4-D were used. When explants were treated individually with growth regulators, the maximum (58.33%) frequency was produced by 4 mg/l 2,4-D. The BAP was completely ineffective when used individually. The maximum number of an advanced-stage embryo (2.85) was obtained with treatment 0.1 mg/l BAP and 2 mg/l 2,4-D. The maximum plantlets were developed (1.30) when the cotyledonary-staged embryo from 0.1 mg/l BAP and 2 mg/l 2,4-D were transferred to MS basal medium. The maturation and germination of somatic embryos were challenging, suggesting the need for molecular approaches through proteomic expression for mass production and understanding the evolution, structure, and genetic organization of the plant species. The plantlets obtained were acclimatized and survived in green house condition. Histological observation showed that the cells of the globular embryos had a dense cytoplasm and a long suspensor and used to distinguish between embryonic and non-embryonic cells.

show abstract

Analysis of regeneration protocols for micropropagation of Pterocarpus santalinus

Cited by 4 publications

References 50 publications

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

Efficient regeneration of mature castanopsis hystrix from in vitro stem explants

Somatic Embryogenesis and Plantlet Regeneration in Red Sandalwood (Pterocarpus Santalinus)

Contact Info

Product

Resources

About