Analysis of reduced and oxidized antioxidants in Hevea brasiliensis latex reveals new insights into the regulation of antioxidants in response to harvesting stress and tapping panel dryness

Junaidi, Junaidi; Nuringtyas, Tri Rini; Clément‐Vidal, Anne; Flori, Albert; Syafaah, Afdholiatus; Oktavia, Fetrina; Ismawanto, Sigit; Aji, Martini; Subandiyah, Siti; Montoro, Pascal

doi:10.1016/j.heliyon.2022.e09840

Cited by 11 publications

(9 citation statements)

References 54 publications

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“…This is supported by our results, as we observed a decrease in the abundance of ROS metabolism‐related proteins and in RSH content in the latex of TPD‐affected trees. Excessive ROS accumulation caused by decreased RSH levels in the laticiferous cells of TPD‐affected trees mainly leads to membrane damage, lutoid bursting, and subsequent in situ latex coagulation (Junaidi et al., 2022 ). However, this is not the major reason for the decline in rubber biosynthesis activity in TPD‐affected trees.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

et al. 2023

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SUMMARY Tapping panel dryness (TPD) is a century‐old problem that has plagued the natural rubber production of Hevea brasiliensis. TPD may result from self‐protective mechanisms of H. brasiliensis in response to stresses such as excessive hormone stimulation and mechanical wounding (bark tapping). It has been hypothesized that TPD impairs rubber biosynthesis; however, the underlying mechanisms remain poorly understood. In the present study, we firstly verified that TPD‐affected rubber trees exhibited lower rubber biosynthesis activity and greater rubber molecular weight compared to healthy rubber trees. We then demonstrated that HbFPS1, a key gene of rubber biosynthesis, and its expression products were downregulated in the latex of TPD‐affected rubber trees, as revealed by transcriptome sequencing and iTRAQ‐based proteome analysis. We further discovered that the farnesyl diphosphate synthase HbFPS1 could be recruited to small rubber particles by HbSRPP1 through protein–protein interactions to catalyze farnesyl diphosphate (FPP) synthesis and facilitate rubber biosynthesis initiation. FPP content in the latex of TPD‐affected rubber trees was significantly decreased with the downregulation of HbFPS1, ultimately resulting in abnormal development of rubber particles, decreased rubber biosynthesis activity, and increased rubber molecular weight. Upstream regulator assays indicated that a novel regulator, MYB2‐like, may be an important regulator of downregulation of HbFPS1 in the latex of TPD‐affected rubber trees. Our findings not only provide new directions for studying the molecular events involved in rubber biosynthesis and TPD syndrome and contribute to rubber management strategies, but also broaden our knowledge of plant isoprenoid metabolism and its regulatory networks.

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Section: Discussionmentioning

confidence: 99%

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

et al. 2023

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“…Interestingly, only one paper by Huang and his team reported the study in metallothioneins (MTs), a ROS scavenger, in response to tapping (Huang et al, 2018). Thiol content, one of the LD parameters indicating stress level, is still poorly interpreted due to the requirement of reference data under low and high harvesting stress for interpretation (Junaidi et al, 2022). Therefore, studies on a simple and robust stress marker are important for mitigating the damaging effect of harvesting practice.…”

Section: Discussionmentioning

confidence: 99%

Research trend in latex harvesting of rubber tree (Hevea brasiliensis Muell. Arg.) based on bibliographic analysis

Junaidi¹

2022

PJA

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The rubber plant (Hevea brasiliensis Muell. Arg.) is the main natural rubber-producing species. Researches on rubber has been carried out for more than a century. Researchers and academics should stay up to date with the current scientific issues including latex harvesting in H. brasiliensis Muell. Arg. This article presents a bibliometric analysis of scientific literature indexed by Scopus and published from 2018 to 2022. The literature was categorized into three sub-topics i.e., latex harvesting techniques (38 papers), physiological mechanisms (41 papers), and oxidative stress induced by latex harvesting practices (18 papers). Metadata validation was performed using Mendeley reference management software and bibliometric analysis was carried out using VOSviewer bibliometric network visualization software. The results on the latex harvesting technique showed that most of the articles were related to the development of automatic tapping machines. This indicates a trend that latex harvesting techniques are likely shifting from manual tapping to fully automated methods using machines and robots. In terms of physiological mechanisms related to latex production, the mechanism of rubber biosynthesis and ethylene response at the genomic, transcriptomic, and proteomic levels predominated the finding. Research on oxidative stress induced by tapping mainly focuses on the effects of mechanical wounding and ethylene stimulation, while research on antioxidants is still limited. In the past five years, biotechnology and molecular analysis are the main tools to study physiological mechanisms and oxidative stress. This can be a consideration for scientists and research institutions to develop laboratories and human resources to be able to conduct molecular-based research.

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“…Tingkat stress tanaman karet melalui pelukaan saat penyadapan hanya dapat diketahui dari perubahan thiol. Pola perubahan kadar thiol diketahui meningkat saat tingkat stress tanaman sementara waktu, lalu menurun dalam jangka waktu lama seiring dengan meningkatnya serangan kering alur sadap (Junaidi et al, 2022).…”

Section: Kondisi Fisiologis Tanamanunclassified

Pengaruh Konsentrasi Stimulan Etefon Terhadap Produksi Dan Fisiologi Lateks Tanaman Karet Pada Awal Buka Sadap Di Areal Yang Mengalami Serangan Penyakit Gugur Daun Sekunder

Andriyanto¹,

SINURAT²,

JUN³

et al. 2023

JPK

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Penggunaan stimulan pada tanaman karet saat awal buka sadap umumnya digunakan untuk memaksimalkan produksi lateks saat penyadapan. Adanya serangan penyakit gugur daun sekunder mengakibatkan kondisi daun gugur dan produksi rendah. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi stimulan saat awal buka sadap pada kondisi gugur daun sekunder. Penelitian dilakukan di Kebun Percobaan Unit Riset Sungei Putih, Pusat Penelitian Karet, Galang, Deli Serdang, Sumatra Utara pada bulan November 2021 sampai Februari 2022. Tanaman karet yang digunakan berumur tujuh tahun pada panel B0-1 (konsumsi kulit terpakai 9,60 cm) dan multiklon (PB 260, PB 340, PB 330, IRR 104, IRR 5, IRR 112, IRR 118, dan BPM 1) dengan interval sadap D3. Rancangan percobaan menggunakan rancangan acak kelompok non faktorial dengan perlakuan 1) tidak diaplikasikan stimulan atau kontrol, 2) aplikasi stimulan konsentrasi 1%, 3) aplikasi stimulan konsentrasi 2%, dan 4) aplikasi stimulan konsentrasi 2,5%. Hasil penelitian menunjukkan bahwa aplikasi konsentrasi stimulan 1% selama empat bulan pada saat awal buka sadap signifikan berbeda nyata terhadap parameter pengamatan produksi, laju aliran lateks, indeks penyumbatan, dan kadar fosfat anorganik. Produksi perlakuan konsentrasi stimulan 1% lebih tinggi 44,11% dibandingkan konsentrasi stimulan 2% dan 48,10% lebih tinggi dibandingkan konsentrasi stimulan 2,5% pada kondisi gugur daun sekunder. Nilai rataan persentase panjang alur sadap yang kering pada perlakuan konsentrasi stimulan 1% sebesar 5,51%. Secara fisiologis, aplikasi konsentrasi stimulan 1% masih aman digunakan pada tanaman dan tidak membuat tanaman tertekan saat terjadi serangan penyakit gugur daun sekunder.

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Analysis of reduced and oxidized antioxidants in Hevea brasiliensis latex reveals new insights into the regulation of antioxidants in response to harvesting stress and tapping panel dryness

Cited by 11 publications

References 54 publications

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

Downregulation of HbFPS1 affects rubber biosynthesis of Hevea brasiliensis suffering from tapping panel dryness

Research trend in latex harvesting of rubber tree (Hevea brasiliensis Muell. Arg.) based on bibliographic analysis

Pengaruh Konsentrasi Stimulan Etefon Terhadap Produksi Dan Fisiologi Lateks Tanaman Karet Pada Awal Buka Sadap Di Areal Yang Mengalami Serangan Penyakit Gugur Daun Sekunder

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