Analysis of red cell parameters on the Sysmex XE 2100 and ADVIA 120 in iron deficiency and in uraemic chronic disease

David, O; Grillo, Antonino; Ceoloni, Barbara; Cavallo, Federica; Podda, G.; Biancotti, P P; Bergamo, Daniela; Canavese, Caterina

doi:10.1080/00365510500406910

Cited by 27 publications

(25 citation statements)

References 26 publications

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“…Ret-He is a measure of the forward scatter of stained reticulocytes and has a curvilinear relationship with CHr. RetHe values can be mathematically converted into reticulocyte hemoglobin concentration values that mirror the CHr values obtained on the Advia analyzers [8][9][10]. The average CHr value for healthy individuals using the Advia analyzer has been reported to be 30.8 pg with no difference between male and female subjects [3].…”

Section: Technical Aspects and Performancementioning

confidence: 99%

Reticulocyte hemoglobin content

2007

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Under normal conditions, reticulocytes are the youngest erythrocytes released from the bone marrow into circulating blood. They mature for 1–3 days within the bone marrow and circulate for 1–2 days before becoming mature erythrocytes. Measurement of cellular hemoglobin concentration has long been reported by automated hematology analyzers as one of the red blood cell indices. The reticulocyte hemoglobin content (CHr or Ret‐He) provides an indirect measure of the functional iron available for new red blood cell production over the previous 3–4 days. Measurement of reticulocyte hemoglobin content in peripheral blood samples is useful for diagnosis of iron deficiency in adults (Mast et al., Blood 2002;99:1489–1491) and children (Brugnara et al., JAMA 1999;281:2225–2230; Ullrich et al., JAMA 2005;294:924–930; Bakr and Sarette, Eur J Pediatr 2006;165:442–445). It provides an early measure of the response to iron therapy increasing within 2–4 days of the initiation of intravenous iron therapy (Brugnara et al., Blood 1994;83:3100–3101). Sequential measurements of reticulocyte hemoglobin content in patients with iron deficiency anemia provide a rapid means for assessing the erythropoietic response to iron replacement therapy (Brugnara et al., Blood 1994;83:3100–3101). It is also an early indicator or iron‐restricted erythropoiesis in patients receiving erythropoietin therapy (Fishbane et al., Kidney Int 1997;52:217–222; Fishbane et al., Kidney Int 2001;60:2406–2411; Mittman et al., Am J Kidney Dis 1997;30:912–922; Tsuchiya et al., Clin Nephrol 2003;59:115–123; Chuang et al., Nephrol Dial Transplant 2003;18:370–377). Thus, reticulocyte hemoglobin content is a recent addition to an expanding list of biomarkers that can be used to differentiate iron deficiency from other causes of anemia. Am. J. Hematol., 2008. © 2007 Wiley‐Liss, Inc.

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Section: Technical Aspects and Performancementioning

confidence: 99%

Reticulocyte hemoglobin content

2007

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“…2 Therefore, human TfS, in direct correlation with serum iron concentration, decreases and remains reduced during ID or inflammation. 5 In cases of ID and concomitant disease such as inflammatory disorders, interpretation of biochemical iron panels is not diagnostically conclusive for ID in people due to complex confounding mechanisms, such as diurnal variation and variability of serum iron [10][11][12] , decreased serum iron due to hepcidin production in inflammatory diseases 13 , and transferrin being a negative acute phase reactant. In addition, TfS is unreliable 12 , and ferritin values can be increased due to the presence of inflammatory cytokines.…”

Section: Introductionmentioning

confidence: 99%

“…5 In cases of ID and concomitant disease such as inflammatory disorders, interpretation of biochemical iron panels is not diagnostically conclusive for ID in people due to complex confounding mechanisms, such as diurnal variation and variability of serum iron [10][11][12] , decreased serum iron due to hepcidin production in inflammatory diseases 13 , and transferrin being a negative acute phase reactant. In addition, TfS is unreliable 12 , and ferritin values can be increased due to the presence of inflammatory cytokines. 13,14 Decreased erythropoiesis during ID, in combination with hemorrhage and a reduced lifespan of iron-deficient RBCs, can result in a complex form of iron deficiency anemia (IDA).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of reticulocyte hemoglobin content (RET‐He) in the diagnosis of iron‐deficient erythropoiesis in dogs

Fuchs

Moritz

Grußendorf³

et al. 2017

Veterinary Clinical Pathol

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Abstract:Background: Reticulocyte hemoglobin content provided by the Siemens ADVIA (CHr) is an established marker of iron deficiency. The IDEXX ProCyte Dx hematology analyzer now provides a similar variable, reticulocyte hemoglobin equivalent (RET-He). Objectives: The objective was to evaluate RET-He and its diagnostic utility in dogs, and to calculate a cutoff value for diagnosing iron-deficient erythropoiesis (IDE). Furthermore, the prevalence of RET-He values below this cutoff value was established. Methods: One hundred and seventy-one CBCs of healthy dogs were used to establish a RI. Stability of RET-He was evaluated by repeated measurements over 48 hours (n = 10). The 25-run coefficient of variation (CV) was calculated, and correlation and bias between measurements of RET-He and CHr were assessed (n = 190). A cutoff value for diagnosing IDE was calculated. The utility of RET-He in the detection of IDE was evaluated in 123 dogs. The prevalence of low RET-He values was assessed retrospectively in a multicenter study (2012)(2013)(2014) under participation of 7 veterinary clinics. Results: Reticulocyte hemoglobin equivalent with an RI of 22.2 to 28.6 pg was statistically stable over 48 hours (P = .10). The CV was 1.8%. A fair correlation (q = 0.74) between RET-He and CHr with a small bias of À0.6 pg was found. The cutoff value for diagnosing IDE was 20.9 pg (sensitivity: 85%; specificity: 99%).

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“…Ret He, generated by all Sysmex XE analysers (Sysmex Corporation, Kobe, Japan), has been recognised as a direct assessment of the incorporation of iron into erythrocyte hemoglobin and a direct estimate of the recent functional availability of iron into the erythron, thus provides the same information as CHr (Thomas et al, 2005;David et al, 2006). Twenty nine pg is the cut off value that defines deficient erythropoiesis Several studies have demonstrated that Ret He and CHr have the same clinical meaning (Mast et al, 2008;Maconi et al, 2009;Miwa et al, 2010).…”

Section: Wwwintechopencommentioning

confidence: 99%