Analysis of recombinant human growth hormone and its related impurities by capillary electrophoresis

CE‐ESI‐TOF/MS for human growth hormone analysis

“…According to the literature, almost every electrophoretic method for the analysis of hGH used basic pH [16,[18][19][20]. Because the protein and the capillary wall were both negatively charged under these conditions, adsorption mediated by electrostatic interactions was reduced.…”

“…CE methods have already been developed for the analysis of hGH and rhGH [16][17][18][19][20] Pharmacopeia incorporated a CE-based approach for detecting charge variants in rhGH [21], while in 2007, an improved method was presented [22]. It included a preconditioning step for dynamically coating the capillary wall with hexadimethrine bromide and poly(vinylsulfonate), which decreased migration time variability.…”

Analysis of recombinant human growth hormone by capillary electrophoresis with bilayer-coated capillaries using UV and MS detection

“…reversedphase [11][12][13][14][15], ion exchange [14,16,17], and size-exclusion LC [12,18,19]. CE also shows great potential for the analysis of rhGH and its degradation products [20][21][22][23][24][25]. Recently, a CE-based method for detection of charge variants in rhGH was incorporated in the European Pharmacopeia (Ph.…”

Impurity analysis of pharmaceuticals using capillary electromigration methods

“…Human growth hormone and four related impurities were separated using CZE. The impurities were quantified in the range of 0.65-2.56% human growth hormone [92]. Impurities of SB-209247, a leukotriene B 4 antagonist, in its final product, were analyzed using MEKC.…”