2000
DOI: 10.1054/bjoc.1999.0995
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Analysis of radon-associated squamous cell carcinomas of the lung for a p53 gene hotspot mutation

Abstract: Summary Squamous cell lung carcinomas (SCC) from former employees of the Wismut uranium mining company (Saxony, Germany) were obtained from the Stollberg Archive in order to screen for p53 tumour suppressor gene codon 249 arg→met hotspot mutations, a putative molecular bio-dosimeter of alpha-particle (radon) exposure (Taylor et al (1994) Lancet 343: 86-87;McDonald et al (1995) Cancer Epidemiol Biomarkers Prevent 4: 791-793). Of the 29 archived samples of SCC meeting quality criteria for DNA analysis by polymer… Show more

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Cited by 18 publications
(11 citation statements)
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“…However, no such mutations were found in this sample set implicating tumor histology as one determinant for the specific mutation [McDonald et al, 1995]. In following studies in lung cancers among uranium miners from Germany [Bartsch et al, 1995;Hollstein et al, 1997;Yang et al, 2000] the specific mutation was described in 2 out of 79 lung cancers, both of them in SCC. This was, however, not significantly different from the frequency of codon 249 mutations in lung cancer in the IARC-based p53 mutation database [Yang et al, 2000].…”
Section: Radonmentioning
confidence: 88%
“…However, no such mutations were found in this sample set implicating tumor histology as one determinant for the specific mutation [McDonald et al, 1995]. In following studies in lung cancers among uranium miners from Germany [Bartsch et al, 1995;Hollstein et al, 1997;Yang et al, 2000] the specific mutation was described in 2 out of 79 lung cancers, both of them in SCC. This was, however, not significantly different from the frequency of codon 249 mutations in lung cancer in the IARC-based p53 mutation database [Yang et al, 2000].…”
Section: Radonmentioning
confidence: 88%
“…Genomic DNA was prepared from the tumor samples as previously described (Yang et al , 2000). PCR amplification was performed with primers previously described for exons 9 and 20 of PIK3CA (Samuels et al , 2004).…”
Section: Methodsmentioning
confidence: 99%
“…Among the 52 studied lung cancers, 16 contained an AGG – ATG transversion leading to an amino acid change (arg to met), distinct from the aflatoxin-related mutation in liver cancers at the same locus (AGG - AGT, arg to ser, (126, 127)). However, other studies of uranium miners with radon exposure (128132), or of other environmentally exposed populations (43, 133, 134) have found very few or no codon 249 AGG-ATG mutations in lung cancers. The predominant type of mutations induced by ionizing radiation in mammalian cells are large deletions or translocations (127).…”
Section: Biomarkers Of Exposurementioning
confidence: 96%