Analysis of Pyrimethanil, Metalaxyl, Dichlofluanid, and Penconazol in Must and Wine from Red Grapes by Solid-Phase Extraction and Gas Chromatography

Abstract

This article describes a method for the simultaneous analysis of four fungicides-pyrimethanil, penconazol, metalaxyl, and dichlofluanid-in must and wine by solid-phase extraction. Two cartridges (octadecylsilane and polystyrenedivinylbenzene) were evaluated with different elution solvents. The most efficient solid phase was octadecylsilane together with ethyl acetate as elution solvent. Determination was performed by gas chromatography with a nitrogen-phosphorus detector, using metribuzin as internal quantification standard. To reduce quantitative errors from matrix effects, matrix-matched calibration was used. The linear regression coefficients were at least 0.9990. The quality parameters of the proposed method presented good recoveries from spiked must and wine, ranging from 92.3 to 105.5%, and good precision, indicated by relative standard deviations (RSDs) of less than 10%. The limits of quantification varied from 0.27 mg L À1 for penconazol in wine to 9.34 mg L À1 for metalaxyl in wine. An additional confirmation method, gas chromatography with mass spectrometry detection, was used.

Determination of fungicide pyrimethanil in grapes, must, fermenting must and wine

“…The LOD and LOQ were estimated as the concentrations obtained with the average noise value plus three and ten times the standard deviation (SD) respectively. For GC/MS the LOD and LOQ were determined as the analyte concentrations that gave signal‐to‐noise (S/N) ratios of 3 and 10 respectively …”

“…The SPE method was developed in our laboratory . SPE cartridges containing 200 mg of C18 sorbent were used.…”

An electrochemical sensor for the determination of environmentally hazardous fungicide pyrimethanil in water and fruit samples

“…Hence, numerous techniques have been implemented to detect pyrimethanil, some of which are gas chromatography, high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry, surface-enhanced Raman spectroscopy and capillary electrophoresis. [6][7][8][9][10] Despite many advantages, all these approaches are associated with some disadvantages such as tedious sample preparation, high cost and limited real-time and on-site potency of determination. [11][12][13][14][15][16] Among these, great attention has been paid to the electrochemical sensors for the quantication of pesticide residues because of their unique features such as cost-effectiveness, rapidity and high sensitivity.…”

“…Hence, numerous techniques have been implemented to detect pyrimethanil, some of which are gas chromatography, high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry, surface-enhanced Raman spectroscopy and capillary electrophoresis. 6–10 Despite many advantages, all these approaches are associated with some disadvantages such as tedious sample preparation, high cost and limited real-time and on-site potency of determination. 11–16…”

Determination of Metalaxyl in Potatoes and Soil by Dispersive Solid-Phase Extraction and High-Performance Liquid Chromatography

“…[2] Various analytical methods have been reported for the determination of metalaxyl in food. Vaquero-Fernández [3] carried out solid-phase extraction (SPE) to detect residues in wine with gas chromatography coupled with nitrogen phosphorus detector. Although the recovery was high (92.3-105.5%), SPE is time-consuming and costly.…”