Analysis of Puumala orthohantavirus Genome Variants Identified in the Territories of Volga Federal District

Abstract

Hemorrhagic fever with renal syndrome (HFRS) is a zoonotic disease commonly diagnosed in the Volga Federal District (VFD). HFRS is caused by Puumala orthohantavirus (PUUV), and this virus is usually detected in bank voles as its natural host (Myodes glareolus). The PUUV genome is composed of the single-stranded, negative-sense RNA containing three segments. The goal of the current study is to identify genome variants of PUUV strains circulating in bank voles captured in the Udmurt Republic (UR) and Ulyanovsk region (ULR). The comparative and phylogenetic analysis of PUUV strains revealed that strains from Varaksino site UR are closely related to strains previously identified in the Pre-Kama area of the Republic of Tatarstan (RT), whilst strains from Kurlan and Mullovka sites ULR are similar to strains from the Trans-Kama area of the RT. It was also found that Barysh ULR strains form a separate distinct group phylogenetically equidistant from Varaksino and Kurlan–Mullovka groups. The identified groups of strains can be considered as separate sub-lineages in the PUUV Russian genetic lineage. In addition, the genomes of the strains from the UR, most likely, were formed as a result of reassortment.

Puumala Orthohantavirus Reassortant Genome Variants Likely Emerging in the Watershed Forests

“…The RUS genetic lineage of PUUV is distributed in VFD and other regions of European Russia. In contrast, the FIN genetic lineage is isolated from bank voles in Karelia and western Siberia [ 6 , 12 , 13 , 14 , 15 , 16 ]. Although much is learned about PUUV strains circulating in Russia, there is a need to understand better the correlation between PUUV strains isolated from HFRS patients and bank voles from the same area [ 17 , 18 ].…”