Analysis of purines and pyrimidines by mixed partition–adsorption normal-phase high-performance liquid chromatography

Kažoka, H.

doi:10.1016/s0021-9673(01)01397-8

Cited by 17 publications

(8 citation statements)

References 41 publications

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“…We suggest that this observation may be explained by limited miscibility of ethanolamine with hexane-based mobile phase. It is known that stationary liquid phase can be generated spontaneously on the surface of a stationary phase even by mobile phases which are not completely saturated with polar components [36]. In this case, low proportion of 2-propanol in mobile phase may cause formation of certain liquid stationary phase on the CSP thus blocking the active sites of sorbent and resulting in lower retention factor values.…”

Section: Effect Of the Percentage Of 2-propanolmentioning

confidence: 99%

Enantioseparation of 1-Phenyl-1,2,3,4-tetrahydroisoquinoline on Polysaccharide-Based Chiral Stationary Phases

2011

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The enantiomers of 1-phenyl-1,2,3,4-tetrahydroisoquinoline have been directly separated on polysaccharide-based chiral stationary phases (CSPs). The normal phase separation of (S)-and (R)-1-phenyl-1,2,3,4-tetrahydroisoquinoline was accomplished by screening of the immobilized Chiralpak IC column with different eluents. The effect of mobile phase type on retention, selectivity and resolution was studied. 2-Propanol or ethanol/n-hexane/ ethanolamine mixtures were applied as mobile phases by screening of following polysaccharide-based immobilized (Chiralpak IA, Chiralpak IC) and coated (Lux Cellulose-1, Lux Cellulose-2, Lux Amylose-2) CSPs. Polar organic and reversed-phase conditions were also tested for direct enantioseparation of 1-phenyl-1,2,3,4-tetrahydroisoquinoline.

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Section: Effect Of the Percentage Of 2-propanolmentioning

confidence: 99%

Enantioseparation of 1-Phenyl-1,2,3,4-tetrahydroisoquinoline on Polysaccharide-Based Chiral Stationary Phases

2011

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“…Equilibrate the chromatographic column with Buffer A for 20 min at 1.2 mL/min and 10 • C prior to the injection of any standards or biological sample. (5), hypoxanthine (6), thymine (7), xanthine (8), ascorbic acid (9), uridine (10), adenine (11), SAM (14), inosine (15), uric acid (16) guanosine (17), thymidine (18) orotic acid (19), SAH (24), AICAR (25), adenosine (26), SAICAR (28), succinyladenosine (29), and adenylosuccinate (31) was performed at 260 nm. In Panel B, the assignment of peaks to propionic acid (12), GSH (13), malonic acid (20), methylmalonic acid (21), NAA (22), NAG (23), GSSG (27), and NAAG (30) was performed at 206 nm.…”

Section: Hplc Equipment and Chromatographic Run Conditionsmentioning

confidence: 99%

HPLC Analysis for the Clinical–Biochemical Diagnosis of Inborn Errors of Metabolism of Purines and Pyrimidines

Lazzarino

Amorini

Pietro

et al. 2010

Methods in Molecular Biology

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The determination of purines and pyrimidines in biofluids is useful for the clinical-biochemical characterization of acute and chronic pathological states that induce transient or permanent alterations of metabolism. In particular, the diagnosis of several inborn errors of metabolism (IEMs) is accomplished by the analysis of circulating and excreted purines and pyrimidines. It is certainly advantageous to simultaneously determine the full purine and pyrimidine profile, as well as to quantify other compounds of relevance (e.g., organic acids, amino acids, sugars) in various metabolic hereditary diseases, in order to screen for a large number of IEMs using a reliable and sensitive analytical method characterized by mild to moderate costs. Toward this end, we have developed an ion-pairing HPLC method with diode array detection for the synchronous separation of several purines and pyrimidines. This method also allows the quantification of additional compounds such as N-acetylated amino acids and dicarboxylic acids, the concentrations of which are profoundly altered in different IEMs. The application of the method in the analysis of biological samples from patients with suspected purine and pyrimidine disorders is presented to illustrate its applicability for the clinical-biochemical diagnosis of IEM.

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“…Besides the abovementioned procedures, alternative separation techniques have been developed too. Kazoka et al used normal phase liquid chromatography (NPLC) with the mixed partition-adsorption (MPA) mode as an alternative approach for the purines and pyrimidines determination [15]. Furthermore, the liquid chromatography based on hydrophobic interactions was described too [16].…”

Section: Introductionmentioning

confidence: 99%

Separation of Nucleobases Using High‐performance Liquid Chromatography Coupled with Voltammetric Scanning

et al. 2018

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In this paper, a method based on chromatographic separation of analytes and their quantification using on‐line cyclic voltammetry is reported. The method based on high performance liquid chromatography on reverse phase column was optimized using free nucleobases‐guanine, adenine, thymine, and cytosine. The optimal separation of nucleobases was detected when using 0.05 M borate buffer (pH 9.0) as the mobile phase, at isocratic flow rate 1 mL min−1, and at separation column temperature of 30 °C. The accurate timing of the cyclic voltammetry enabled us to quantify the concentrations of individual nucleobases by oxidation on glassy carbon electrode.

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Analysis of purines and pyrimidines by mixed partition–adsorption normal-phase high-performance liquid chromatography

Cited by 17 publications

References 41 publications

Enantioseparation of 1-Phenyl-1,2,3,4-tetrahydroisoquinoline on Polysaccharide-Based Chiral Stationary Phases

Enantioseparation of 1-Phenyl-1,2,3,4-tetrahydroisoquinoline on Polysaccharide-Based Chiral Stationary Phases

HPLC Analysis for the Clinical–Biochemical Diagnosis of Inborn Errors of Metabolism of Purines and Pyrimidines

Separation of Nucleobases Using High‐performance Liquid Chromatography Coupled with Voltammetric Scanning

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