Analysis of pure pancreatic juice proteins by two-dimensional gel electrophoresis in cases of pancreatic cancer

Abstract

To clarify the difference in the protein composition of pancreatic juice between patients with pancreatic cancer and normal controls, the proteins of pure pancreatic juice from three cases of cancer of the head of the pancreas and six apparently healthy adults were analyzed by two-dimensional gel electrophoresis (two-DE) followed by silver staining. Two minor proteins of Mr 59,000 and Mr 78,000 present in all the three patients were not detected in the six controls. We have identified the minor proteins with Mr 59,000 and Mr 78,000 as alpha 1-antitrypsin and transferrin, respectively, using Western blotting with anti-alpha 1-antitrypsin and anti-transferrin antibodies. In addition, serum albumin also identified by antibody binding was abundantly present in patients compared to controls. The increase in the amount of serum albumin in the patient was also confirmed by a quantitative analysis using SDS-PAGE and gel densitometry. The data indicate that not only serum albumin but also alpha 1-antitrypsin and transferrin are increased in the pancreatic juice of the patients with pancreatic cancer, as compared with apparently healthy adults. The data also suggests that the analysis of pancreatic juice proteins by two-DE with silver staining is useful for the diagnosis of pancreatic diseases.

ITRAQ-based quantitative proteomics reveals apolipoprotein A-I and transferrin as potential serum markers in CA19-9 negative pancreatic ductal adenocarcinoma

“…[ 27 ] Furthermore, it was reported that TF was increased in the pancreatic juice of the patients with pancreatic cancer, as compared with apparently healthy adults. [ 28 ] Based on findings of our study, we guess that TF is secreted to pancreatic juice rather than blood in patients with PDAC. The finding that TF expression correlated with differentiation of the tumor and the survival further confirmed the importance of this protein in association with PDAC.…”

The distinctive gastric fluid proteome in gastric cancer reveals a multi-biomarker diagnostic profile

“…Furthermore, many cancers share the same markers e.g. CEA, CA19-9, transferrin and alpha1-antitrypsin [40,42,64].…”

Phaseolus vulgaris Erythroagglutinin (PHA-E)-Positive Ceruloplasmin Acts as a Potential Biomarker in Pancreatic Cancer Diagnosis

“…Among these proteins, we noticed that three proteins, Cp, Apo-E, and Tf, had relative higher protein scores according to nanoLC-MS/MS analysis and their molecular weights (MWs) were well-corresponded with the bands in the gel ( Figure 3 a). Cp, Apo-E, and Tf were reported as pivotal oncogenic proteins involved in the progression of PC and have the potential for PC diagnosis [ 22 , 23 , 27 , 28 ]. Therefore, we further analyzed the level of Cp, Apo-E, and Tf in PHA-E pulled-down serum proteins by using their specific antibodies though Western blotting.…”