Analysis of pulmonary allergic vasculitis with eosinophil infiltration in asthma model of mice

Abstract

Here the authors report pulmonary allergic vasculitis with eosinophil infiltration in an asthma model of mice and investigated its pathogenesis. C57BL/6 and BALB/c mice were sensitized with ovalbumin (OVA). After the inhalation of OVA, the authors measured the cell number and cytokine concentration in the blood and bronchoalveolar lavage fluid (BALF). The authors also examined the histological changes of the pulmonary. The number of eosinophils increased in the blood and BALF in both strains; however, the number in C57BL/6 in BALF was significantly higher than that in BALB/c. Histological analysis demonstrated severe vasculitis of the pulmonary arteries with derangement of the muscle layer and smooth muscle cell hyperplasia in C57BL/6. Semiquantitative analysis of the severity of vasculitis in the pulmonary arteries revealed that the internal vascular space was highly reduced by smooth muscle hyperplasia in C57BL/6 compared to BALB/c mice. The concentrations of interleukin (IL)-4, IL-5, and interferon (IFN)-gamma in BALF of C57BL/6 were significantly high compared to those of BALB/c. C57BL/6 mice exhibited severe allergic vasculitis in the pulmonary arteries compared to BALB/c mice. The high concentrations of IL-4, IL-5, and IFN-gamma in the lung may play a critical role in the pathogenesis of allergic vasculitis in C57BL/6 mice.

Airport emission particles: exposure characterization and toxicity following intratracheal instillation in mice

“…Histological examination of lung and liver tissue revealed eosinophilic pulmonary vasculitis in CAP-exposed mice, likely reflecting the exposure to pollen grains, which can be associated with allergic response. This type of histopathology was previously reported in association with asthma models in mice [55]. To the best of our knowledge this has previously not been reported in association with particle exposures.…”

Effects of imatinib mesylate on pulmonary allergic vasculitis in a murine model

“…Drastic obstructive remodeling of small‐sized pulmonary arteries was observed on the seventh day in the OVA‐sensitized mice stimulated with repetitive OVA inhalation. The intraluminally accumulated cells were myofibroblasts which are spindle‐shaped cells, and were positively stained with anti‐actin antibody . These cells expressed Ki‐67, suggesting that they were proliferating cells.…”

“…In the present study, we used the murine model of pulmonary allergic vasculitis which we previously reported as an animal model of AGA . The following histopathological changes in our murine model resembled allergic granulomatous angiitis in humans: (i) infiltration of mononuclear cells and eosinophils, and granuloma with multinuclear giant cells in the arterial wall; (ii) disruption of internal elastic layer; and (iii) obliteration of pulmonary arteries by mesenchymal cells.…”

“…The extent of histological changes in pulmonary arteries was assessed as previously described . In brief, the tissue was cut into sections 3 m thick, stained with hematoxylin and eosin and evaluated by light microscopy.…”

“…We previously reported a murine model of pulmonary allergic vasculitis, which was induced by repeated inhalation of ovalbumin (OVA) in C57BL/6 mice sensitized with OVA . We observed that small pulmonary arteries were occluded with accumulated myofibroblasts and collagen deposition on the seventh day.…”