Analysis of protein components at varioue stages of Clonorchis sinensis

Abstract

In this study the authors examined the protein components at various stages of Clonorchis sinensis, and those of tegument and metabolite of adult Clonorchis by using SDS-polyacrylamide gel electrophoresis and immunodiffusion. The following results were obtained: 1. The protein components of C. sinensis were gradually changed during its development. A considerable change occurred during the initial 7 days after the metacercarial infection. 2. Two bands of protein of about 97,000 molecular weight (MW) and 178,000 MW were unique to excysted metacercaria of C. sinensis. Other 2 bands of protein of 23,000 and 25,000 MW which were absent in metacercariae, might be associated with the development of sex organs in adult. 3. In the metabolite, some components of tegumental proteins were detected. And this tegumental protein components in metabolite seems to be the major antigenic components reacting with infected rabbit antiserum by immunodiffusion. 4. Twenty bands of protein were detected in the isolated adult tegument. Among them 6 bands were in 97,000~65,000 MW, 3 bands in 56,000~53,000 MW and 5 bands in 37,000~30,000 MW. On the other hand, in metabolic products of adult C. sinensis, 17 bands were detected.

A novel tegumental protein 31.8 kDa of Clonorchis sinensis: sequence analysis, expression, and immunolocalization

“…Many proteins identified in the tegument of S. japonicum, S. mansoni, and F. gigantica were potentially molecules for vaccine development and for immunological diagnosis (Jeffs et al 1991;Waine et al 1994;Xavier et al 1998). It was documented that the tegument showed the strongest staining in the immunohistochemical staining of C. sinensis with C. sinensis-infected animal sera (Cho et al 1986;Choi et al 1981;Hong et al 2001). The transcripts of CsTP31.8 were detected in the stage of metacercariae and adult worm, so that if CsTP31.8 might be defined as an important antigen of C. sinensis, it could be developed as a vaccine not only for intermediate host but also for definitive host.…”